广西师范大学学报（自然科学版） ›› 2022, Vol. 40 ›› Issue (2): 231-241.doi: 10.16088/j.issn.1001-6600.2020112002

• • 上一篇    下一篇

AMF与根瘤菌对间作大豆光合与呼吸代谢的影响

吴艳芬1,2, 刘秋鸣1,2, 刘卫欢1,2, 蒙爱萍1,2, 陈振翔1,2, 刘灵1,2*   

  1. 1.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学),广西 桂林 541006;
    2.广西师范大学 生命科学学院,广西 桂林 541006
  • 收稿日期:2020-11-20 修回日期:2021-04-01 发布日期:2022-05-31
  • 通讯作者: 刘灵(1968—),女,广西桂林人,广西师范大学副教授,博士。E-mail:liuling@mailbox.gxnu.edu.cn
  • 基金资助:
    国家自然科学基金(31460049);广西自然科学基金(联合资助培育项目2018GXNSFAA138001);广西高校科学技术研究重点项目(ZD2014015)

Effects of Inoculation of AMF and Rhizobium on Photosynthetic and Respiratory Metabolism and Growth of Intercropping Glycine max

WU Yanfen1,2, LIU Qiuming1,2, LIU Weihuan1,2, MENG Aiping1,2, CHEN Zhenxiang1,2, LIU Ling1,2*   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Spices and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2020-11-20 Revised:2021-04-01 Published:2022-05-31

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摘要： 为探索接种幼套球囊霉Glomus etunicatum(简写为A)和费氏中华根瘤菌Sinorhizobium fredii(简写为R)对金橘Fortunella margarita (Lour.) Swingle(简写为F)/大豆Glycine max(简写为S)间作系统中大豆植株生长的影响,本文通过田间试验,分析间作系统中各处理大豆的光合与呼吸代谢及植株生长发育状况。结果表明:1)所有间作处理的大豆根系菌根侵染强度ηm和根系泡囊强度ηV均比相应的单作处理低,各相对应处理间ηm差异均显著,ηV差异均不显著(除间作双接种处理较单作双接种处理ηV显著增加外)。与相应的S或F+S对照相比,所有单接种A或单接种R处理分别表现出显著或不显著的促进效应,而双接种处理的增加效应最大且差异显著。2)间作F+S处理的大豆根瘤数量和干质量均比相应的单作S处理低,但分别单接种A或R处理后,均呈增加趋势;单作系统和间作系统中,双接种A+R处理的促进效果最显著。3)间作系统中,F+S处理的大豆叶片叶绿素含量和净光合速率均比相应的单作S处理低,分别单接种A或R后均呈升高趋势,双接种处理的促进效果优于单接种处理,间作双接种处理的净光合速率最高。4)间作系统的大豆叶片琥珀酸脱氢酶活性高于相应的单作处理,接种处理的促进效果由大到小均为双接种A+R、单接种A、单接种R。5)大豆叶片线粒体膜H+-ATP酶活性的变化趋势与净光合速率的变化趋势相似。6)各间作接种处理的大豆生物量及产量均高于其相对应的单作接种处理,且各间作接种处理均有A+R>A>R的促进趋势。可见,幼套球囊霉和费氏中华根瘤菌双接种处理可显著促进间作大豆植株生长,促进效应优于单接种处理。

关键词: 幼套球囊霉, 费氏中华根瘤菌, 间作大豆, 光合生理, 呼吸代谢

Abstract: In order to explore the effects of inoculation of Glomus etunicatum (abbreviated as A, the similar as below) and Sinorhizobium fredii (R) on plant growth of soybean (Glycine max)in kumquat (Fortunella margarita (Lour.) Swingle (F)/soybean (S) intercropping system, a series of field experiments were conducted to analyze the photosynthetic and respiratory metabolism and plant growth of S inoculated with several treatments. The results showed that: 1)The mycorrhizal infection intensity ηm and vesicle intensity ηV in root system of S of all intercropping treatments were lower than those of monocropping treatments respectively, there were significant differences in ηm and no significant differences in ηV among the corresponding treatments (except that ηV increased significantly in intercropping double inoculation treatment compared with monoculture double inoculation treatment). Compared with the corresponding S or F+S treatment, those of all single inoculation A treatments or single inoculation R treatments showed significant or unsignificant promoting effects respectively, while the increase effect of ηm or ηV of double inoculation treatment A+R was the largest and the differences was significant respectively. 2)Compared with the corresponding monocropping treatments, the number of nodule and dry weight of S were decreased in intercropping system. Compared with the corresponding single inoculation A or R treatments, the promotion with A+R treatment were the most significant respectively. 3)The chlorophyll content and net photosynthetic rate of S were decreased with F+S treatment, while increased after inoculated with single A or R treatment. Those of the promotion effect of both A and R treatment was better than single A or R treatment. 4)The activity of succinate dehydrogenase of S in intercropping system were higher than that of monoculture system, and the promoting effect sequence of inoculation treatment was A+R>A>R. 5)The change trend of H+-ATPase activity in mitochondrial membrane of S leaves was similar to that of net photosynthetic rate. 6)The soybean biomass and yield of the intercropping inoculation treatment were higher than the monocropping inoculation treatment, and the inoculation treatments showed a decreasing trend of A+R>A>R. Therefore, treatment inoculated with both A and R could promote significantly the growth of S in intercropping system, the promotion effect was better than that of the single inoculation treatment.

Key words: arbuscular mycorrhizal fungi (Glomus etunicatum), rhizobium (Sinorhizobium fredii), intercropping soybean (Glycine max), photosynthetic physiology, respiratory metabolism

中图分类号: 

  • S565.1
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