青钱柳叶多糖中单糖组分分析

doi:10.16088/j.issn.1001-6600.2018.02.012

摘要/Abstract

摘要： 采用水提醇沉法提取青钱柳叶中粗多糖，用苯酚-硫酸法测定多糖含量，进一步采用柱前衍生 HPLC 法测定多糖中的单糖组成，并对3个不同来源的青钱柳叶中多糖和单糖的含量与构成进行比较分析。研究结果显示，青钱柳叶中粗多糖的平均质量分数为4.34%，青钱柳叶中多糖主要由木糖、甘露糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖和核糖等单糖组成，其在粗多糖中的质量分数分别为 3.04%、7.35%、8.46%、13.46%、24.32%、24.26%和6.16%。青钱柳叶中多糖因青钱柳叶来源不同而存在单糖组成差异。本研究为青钱柳叶质量控制与药理药效研究提供了依据。

关键词: 青钱柳, 柱前衍生法, HPLC, 多糖, 单糖组成

Abstract: The polysaccharides were extracted by water extraction and alcohol precipitation method. Phenol sulfuric method was used for the determination of the content of polysacchrides. The monosacchride constituents of polysacchrides in the leaf of [WTBX]Cyclocarya paliurus[WTBZ] were determined by pre column derivatization HPLC. The content and composition of polysaccharides and monosaccharide from three different sources of Cyclocarya paliurus were analyzed and compared. The results displayed an average content of 4.34% for polysaccharides. The monosaccharide constituents of polysaccharides from the leaf of Cyclocarya paliurus are xylose, fructose, rhamnose, arabinose, galactose, glucose and ribose with their contents in polysaccharides being 3.04%，7.35%，8.46%，13.46%，24.32%，24.26% and 6.16%, respectively. The monosaccharide constituents and the content of polysaccharides show differences among different sources of Cyclocarya paliurus . It provides a basis for quality control and pharmacological efficacy study of Cyclocarya paliurus.

Key words: Cyclocarya paliurus, pre column derivatization method, HPLC, polysaccharides, monosaccharide constituents

中图分类号:

Q532

蒋向辉, 蒋天智, 王翔, 苑静. 青钱柳叶多糖中单糖组分分析[J]. 广西师范大学学报（自然科学版）, 2018, 36(2): 87-93.

JIANG Xianghui, JIANG Tianzhi, WANG Xiang, YUAN Jing. Monosaccharide Constituents of the Polysaccharides from the Leaf of Cyclocarya paliurus[J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(2): 87-93.

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