Journal of Guangxi Normal University(Natural Science Edition) ›› 2016, Vol. 34 ›› Issue (1): 118-121.doi: 10.16088/j.issn.1001-6600.2016.01.018

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Simultaneously Determination of Three Effective Componentsin Swertia tetraptera Maxim.

ZHAO Ying1,2,3, LIU Xiaocui1,2,3, LIAO Zhiming1,2,3, LU Yongchang1,2,3   

  1. 1. College of Pharmacy,Qinghai University for Nationalities,Xining Qinghai 810007,China;
    2. Key Laboratory of PlantResources of Qinghai-Tibet Plateau in Chemical Research,Qinghai University for Nationalities,Xining Qinghai 810007,China;
    3. Key Laboratory of Pharmaceutical Analysis in Qinghai Province,Xining Qinghai 810007,China
  • Received:2015-10-29 Published:2018-09-14

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Abstract: RP-HPLC was used to determine the quantity and quality of swertiamarin,gentiopicroside and sweroside in Swertia tetraptera Maxim. The results showed that three compounds were base-isolated. The linear ranges of swertiamarin,gentiopicroside and sweroside were 0.052～1.030 μg (R=0.999 8), 0.060～1.200 μg (R=0.999 9), and 0.050～0.99 μg(R=0.999 8) respectively,the average recoveries were 99.1% (RSD=1.94%), 101.5% (RSD=2.05%), and 96.0% (RSD=1.94%) respectively. This method is simple,rapid and reliable with good reproducibility,and can be used as the determination method for swertiamarin,gentiopicroside and sweroside in Swertia tetraptera Maxim.

Key words: RP-HPLC, swertiamarin, gentiopicroside, sweroside, Swertia tetraptera Maxim.

CLC Number: 

  • O415
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