Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (5): 143-151.doi: 10.16088/j.issn.1001-6600.2022091802

Previous Articles     Next Articles

Research on Luring Effect of 8 Metabolites on Procambarus clarkii

MO Lili1,2,3, ZHOU Zihao1,2,3, LI Dinghong3, ZENG Wenlong3, WU Zhengjun1,2,3, HUANG Jinlong1,2,3,4*, SUN Tao3*   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. Guangxi Key Laboratory of Rare and Endangered Animal Ecology (Guangxi Normal University), Guilin Guangxi 541006, China;
    3. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    4. Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), Guilin Guangxi 541006, China
  • Received:2022-09-18 Revised:2022-11-16 Published:2023-10-09

PDF (PC)

115

Abstract: Pheromones can be recognized by Procambarus clarkii in water for courtship. Clarifying the effective components of P. clarkii pheromones can provide a theoretical basis for green management of aquatic invasive P. clarkii. In this research, the effects of eight standard compounds, namely 4-thiouridine, bilirubin, ethinyl estradiol, 17α-hydroxyprogesterone caproate, N-acetyl-L-leucine, GTP disodium salt, and GDP disodium salt and GMP disodium salt, on the selection behavior of P. clarkii were tested using a Y-shaped water maze, and then screened for the eliciting effect of ethinyl estradiol and 17α-hydroxyprogesterone caproate to prepare concentration assays. The results showed that the male P. clarkii showed nearly 50% selective response rate to 4-thiouridine, bilirubin, N-acetyl-L-leucine, GTP disodium salt, and GDP disodium salt and GMP disodium salt, with selection coefficients between ±0.15 and insignificant luring effects (P>0.05 ). P. clarkii showed high selection response rates to ethinyl estradiol (64.10%) and 17α-hydroxyprogesterone caproate (63.24%) with selection coefficients of 0.28 and 0.26, which showed significant sex differences. The minimum effective luring threshold of ethinyl estradiol and 17α-hydroxyprogesterone caproate to male P. clarkii were 10 and 100 μmol/L, respectively, while female P. clarkii were not sensitive to both compounds. Thus, ethinyl estradiol and 17α-hydroxyprogesterone caproate had significant luring effects on adult male P. clarkii but no significant response to female crayfish, which tentatively showed that ethinyl estradiol and 17α-hydroxyprogesterone caproate were the active components of the sex pheromone in P. clarkii.

Key words: sex pheromone, Procambarus clakii, ethinyl estradiol, 17α-hydroxyprogesterone caproate, Y-shaped maze

CLC Number:  S917.4
[1] 周子豪, 巫虹颖, 黄锦龙, 等. 十足目甲壳类动物化学通讯的研究进展[J]. 生态学杂志, 2022, 41(10): 2050-2057. DOI: 10.13292/j.1000-4890.202210.016.
[2] DERBY C D, HUTSON M, LIVERMORE B A, et al. Generalization among related complex odorant mixtures and their components: analysis of olfactory perception in the spiny lobster[J]. Physiology & Behavior, 1996, 60(1): 87-95. DOI: 10.1016/0031-9384(95)02237-6.
[3] BERRY F C, BREITHAUPT T. To signal or not to signal? Chemical communication by urine-borne signals mirrors sexual conflict in crayfish[J]. BMC Biology, 2010, 8: 25. DOI: 10.1186/1741-7007-8-25.
[4] LIU X, GUO Z W, KE Z W, et al. Increasing potential risk of a global aquatic invader in Europe in contrast to other continents under future climate change[J]. PLoS One, 2011, 6(3): e18429. DOI: 10.1371/journal.pone.0018429.
[5] LOUREIRO T G, ANASTÁCIO P M S G, ARAUJO P B, et al. Red swamp crayfish: Biology, ecology and invasion - an overview[J]. Nauplius, 2015, 23(1): 1-19. DOI: 10.1590/s0104-64972014002214.
[6] BREITHAUPT T. Fan organs of crayfish enhance chemical information flow[J]. Biological Bulletin, 2001, 200(2): 150-154. DOI: 10.2307/1543308.
[7] MOORE P A, EDWARDS D, JURCAK-DETTER A, et al. Spatial, but not temporal, aspects of orientation are controlled by the fine-scale distribution of chemical cues in turbulent odor plumes[J]. Journal of Experimental Biology, 2021, 224(7): jeb240457. DOI: 10.1242/jeb.240457.
[8] REIDENBACH M A, KOEHL M A R. The spatial and temporal patterns of odors sampled by lobsters and crabs in a turbulent plume[J]. Journal of Experimental Biology, 2011, 214(Pt18): 3138-3153. DOI: 10.1242/jeb.057547.
[9] GHERARDI F. Understanding the impact of invasive crayfish[M]// GHERARDI F. Biological Invaders in Inland Waters: Profiles, Distribution, and Threats. Dordrecht: Springer, 2007: 507-542. DOI: 10.1007/978-1-4020-6029-8_28.
[10] JUSSILA J, EDSMAN L, MAGUIRE I, et al. Money kills native ecosystems: European crayfish as an example[J]. Frontiers in Ecology and Evolution, 2021, 9: 648495. DOI: 10.3389/fevo.2021.648495.
[11] KRIEG R, KING A, ZENKER A. Measures to control invasive crayfish species in Switzerland: a success story? [J]. Frontiers in Environmental Science, 2020, 8: 609129. DOI: 10.3389/fenvs.2020.609129.
[12] AQUILONI L, GHERARDI F. The use of sex pheromones for the control of invasive populations of the crayfish Procambarus clarkii: a field study[J]. Hydrobiologia, 2010, 649(1): 249-254. DOI: 10.1007/s10750-010-0253-4.
[13] SOLARI P, PEDDIO S, SOLLAI G, et al. Development of PVC dispensers for long-lasting release of attractants for the control of invasive crayfish populations[J]. Diversity, 2018, 10(4): 128. DOI: 10.3390/d10040128.
[14] HAZLETT B A. Responses to multiple chemical cues by the crayfish Orconectes virilis[J]. Behaviour, 1999, 136(2): 161-177. DOI: 10.1163/156853999501261.
[15] AQUILONI L, MASSOLO A, GHERARDI F. Sex identification in female crayfish is bimodal[J]. Naturwissenschaften, 2009, 96(1): 103-110. DOI: 10.1007/s00114-008-0458-9.
[16] 周丽梅, 鞠倩, 曲明静, 等. 暗黑鳃金龟对性信息素的触角电生理及行为反应[J]. 昆虫学报, 2009, 52(2): 121-125. DOI: 10.16380/j.kcxb.2009.02.001.
[17] 丁红建, 郭予元,吴才宏. 用于昆虫嗅觉行为研究的四臂嗅觉仪的设计、制作和应用[J]. 昆虫知识, 1996, 33(4): 241-243.
[18] BELANGER R M, CORKUM L D. Review of aquatic sex pheromones and chemical communication in anurans[J]. Journal of Herpetology, 2009, 43(2): 184-191. DOI: 10.1670/08-054R1.1.
[19] EAP D, CORREA D, NGO-VU H, et al. Chemosensory basis of feeding behavior in pacific white shrimp, Litopenaeus vannamei[J]. Biological Bulletin, 2020, 239(2): 115-131. DOI: 10.1086/710337.
[20] SWETHA C H, GIRISH B P, REDDY P S. Elucidation of the role of estradiol and progesterone in regulating reproduction in the edible crab, Oziothelphusa senex senex[J]. RSC Advances, 2016, 6(30): 24959-24967. DOI: 10.1039/C5RA23637A.
[21] ZMORA N, CHUNG J S. A novel hormone is required for the development of reproductive phenotypes in adult female crabs[J]. Endocrinology, 2014, 155(1): 230-239. DOI: 10.1210/en.2013-1603.
[22] HARLIOĞLU M M, YONAR M E, HARLIOĞLU A G, et al. Effects of 17β-estradiol injection on the reproductive efficiency of freshwater crayfish, Astacus leptodactylus (Eschscholtz, 1823)[J]. Journal of Applied Aquaculture, 2018, 30(3): 197-210. DOI: 10.1080/10454438.2018.1426515.
[23] SAARISTO M, JOHNSTONE C P, XU K, et al. The endocrine disruptor, 17α-ethinyl estradiol, alters male mate choice in a freshwater fish[J]. Aquatic Toxicology, 2019, 208: 118-125. DOI: 10.1016/j.aquatox.2019.01.006.
[24] JASER W, SEVERIN G F, JÜTTING U, et al. Effects of 17α-ethinylestradiol on the reproduction of the cladoceran species Ceriodaphnia reticulata and Sida crystallina[J]. Environment International, 2003, 28(7): 633-638. DOI: 10.1016/s0160-4120(02)00101-0.
[25] KOBAYASHI M, KIJIMA M, MATSUZUKA Y, et al. Suppression of male reproductive activity by estradiol in goldfish: Appearance of infertile males that perform active spawning behavior[J]. Fisheries Science, 2021, 87(1): 93-104. DOI: 10.1007/s12562-020-01487-1.
[26] SHUI Y, ZHAN M, LI J J, et al. Development of an in vitro culture system for immature oocytes of red swamp crayfish (Procambarus clarkii)[J]. Animal Reproduction Science, 2022, 243: 107005. DOI: 10.1016/j.anireprosci.2022.107005.
[27] VAN DER KRAAK G, SORENSEN P W, STACEY N E, et al. Periovulatory female goldfish release three potential pheromones: 17α,20β-dihydroxyprogesterone, 17α, 20β-dihydroxyprogesterone glucuronide, and 17α-hydroxyprogesterone[J]. General and Comparative Endocrinology, 1989, 73(3): 452-457. DOI: 10.1016/0016-6480(89)90202-5.
[28] OYAMA T, MOMOHARA Y, YANO H, et al. Sex recognition and agonistic strategies of male and female crayfish[J]. Behaviour, 2020, 157(6): 575-596. DOI: 10.1163/1568539X-bja10014.
[29] DUNHAM D W, OH J W. Chemical sex discrimination in the crayfish Procambarus clarkii: Role of antennules[J]. Journal of Chemical Ecology, 1992, 18(12): 2363-2372. DOI: 10.1007/bf00984955.
[30] 寇祥明, 张家宏, 王守红, 等. 克氏原螯虾交配行为的初步研究[J]. 江西农业学报, 2010, 22(12): 150-152, 156. DOI: 10.19386/j.cnki.jxnyxb.2010.12.046.
[31] 吴学三, 郭峰, 田翙, 等. 白星花金龟对9种植物的趋避行为[J]. 植物保护, 2021, 47(6): 141-147. DOI: 10.16688/j.zwbh.2020485.
[32] SONG X R, YE H Z, JIN F F, et al. Effects of fermented soybean meal and guanosine 5′-monophosphate on growth, intestinal health and anti-stress capability of Penaeus vannamei in low fish meal diet[J]. Aquaculture, 2022, 548(Part 1): 737591. DOI: 10.1016/j.aquaculture.2021.737591.
[33] IKEDA I, HOSOKAWA H, SHIMENO S, et al. Feeding stimulant activity of nucleotides, tryptophan, and their related compounds for jack mackerel[J]. Nihon Suisan Gakkaishi, 1991, 57(8): 1539-1542. DOI: 10.2331/suisan.57.1539.
[34] ZHANG D, LIU X, HARLEY M A, et al. Uridine-5′-tri-phosphate is a candidate component of the soluble sex pheromone bouquet in a marine shrimp,Lysmata wurdemanni[J]. Marine Ecology Progress Series, 2020, 640: 139-146. DOI: 10.3354/meps13294.
[35] HARDEGE J D, BARTELS-HARDEGE H D, FLETCHER N, et al. Identification of a female sex pheromone in Carcinus maenas[J]. Marine Ecology Progress Series, 2011, 436: 177-189. DOI: 10.3354/meps09226.
[36] 李慧, 尹士采, 郭宗香, 等. 宽胫夜蛾性信息素类似物的合成及其生物活性[J]. 中国农业科学, 2022, 55(9): 1790-1799.
[37] FLETCHER N, TERSCHAK J A, BARTELS-HARDEGE H D, et al. A pheromone bouquet controls the reproductive behaviour of the male shore crab,Carcinus maenas[J]. Aquatic Ecology, 2022, 56(2): 419-427. DOI: 10.1007/s10452-021-09930-w.
[38] HOFFMANN A, BOURGEOIS T, MUNOZ A, et al. A plant volatile alters the perception of sex pheromone blend ratios in a moth[J]. Journal of Comparative Physiology A, 2020, 206(4): 553-570. DOI: 10.1007/s00359-020-01420-y.
[39] YAN J J, ZHANG M D, ALI A, et al. Optimization and field evaluation of sex-pheromone of potato tuber moth,Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae)[J]. Pest Management Science, 2022, 78(9): 3903-3911. DOI: 10.1002/ps.6725.
[1] WU Xia, XU Yilan, WEN Luting, HUANG Yin, QIN Junqi, ZHOU Kangqi, DU Xuesong, CHEN Zhong, PAN Xianhui, BIN Shiyu. Observation on the Composition, Distribution and Pigment Content of Chromatophores in Rice Flower Carp in Quanzhou, China [J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(6): 222-229.
[2] LIU Jingjie, CHU Wuying, ZHU Xin, SUN Yue, XU Yilan, XIE Yandong, BIN Shiyu, ZHANG Jianshe. Embryonic Development Characteristics and Rhythmic Expression Analysis of RORα Gene under Starvation in Siniperca chuatsi [J]. Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(5): 190-197.
[3] BIN Shiyu, LIAO Fang, DU Xuesong, XU Yilan, WANG Xin, WU Xia, LIN Yong. Research Progress on Cold Tolerance of Tilapia [J]. Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(1): 10-16.
[4] ZHANG Fangliang, DENG Xiaohong, ZHU Xiaotong, XU Lei, HU Jiangli, BIN Shiyu. Cloning and Expression Analysis of Ubiquitin-Specific Protease 7 Gene under Starvation in Mandarin Fish (Siniperca chuatsi) [J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(2): 141-147.
[5] XU Lei,CHEN Xiaobao,TANG Chun,HU Jiangli,BIN Shiyu,ZHANG Fangliang. Cloning and Embryonic Development Expression Analysis of the Uncoupling Proteins 3 Gene in Mandarin Fish [J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(1): 92-97.
[6] WANG Kai-zhuo, BIN Shi-yu, LI Hong-hui, LI Yu-long, LIU Zhi-xing, ZHANG Jian-she, CHU Wu-ying. Molecular Cloning and Expression Analysis of Myostatin Gene in Mandarin Fish (Siniperca chuatsi) [J]. Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(4): 109-114.
[7] CHEN Zhong, TANG Zhang-sheng, LIN Yong, YANG Hui-zhan, ZHANG Yong-de, HUANG Yin, PENG Ting, ZHANG Yan. Selection Effects of Cold Tolerance Capacity in Egyptian Strain of Nile Tilapia Three Generation [J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(2): 117-122.
[8] TANG Zhang-sheng, LU Qi-xi, LIN Yong, BIN Shi-yu, PENG Ting, ZHONG Shu-hong, GAN Xi. Tolerance of GIFT Tilapia (Oreochromis niloticus) under DifferentLow Temperature Stress [J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(1): 105-110.
[9] HU Jiangli, XU Lei, XU Yilan, LIU Jingjie, SUN Yue, XIE Yandong, BIN Shiyu. Cloning and Embryonic Developmental Expression Analysis ofFollistatin Gene in Siniperca chuatsi [J]. Journal of Guangxi Normal University(Natural Science Edition), 2020, 38(4): 124-131.
[10] ZHANG Yong-de, LIN Yong, TANG Zhang-sheng, HUANG Yin, YANG Hui-zan, ZENG Lan, CHEN Zhong, PENG Ting, ZHANG Yan. Breeding of Cold Tolerance and Growth Performance of O.mossambicus× O.niloticus Hybrid lines [J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(4): 92-97.
[11] CHEN Dun-xue, LI Yu-long, BIN Shi-yu, NONG Xiao-xian, LIU Xi-liang, CHENG Jia, ZHANG Jian-she, CHU Wu-ying. Characterization of the Complete Mitochondrial Genome Sequence in Siniperca chuatsi [J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(4): 111-116.
[12] LU Qi-xi, LIN Yong, BIN Shi-yu, TANG Zhang-sheng, PENG Ting. Analysis of Cold Tolerance Measure at Low-temperature in Six Tilapia Families [J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(2): 104-109.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] DONG Shulong, MA Jiangming, XIN Wenjie. Research Progress and Trend of Landscape Visual Evaluation —Knowledge Atlas Analysis Based on CiteSpace[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 1 -13 .
[2] GUO Jialiang, JIN Ting. Semantic Enhancement-Based Multimodal Sentiment Analysis[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 14 -25 .
[3] WU Zhengqing, CAO Hui, LIU Baokai. Chinese Fake Review Detection Based on Attention Convolutional Neural Network[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 26 -36 .
[4] LIANG Zhengyou, CAI Junmin, SUN Yu, CHEN Lei. Point Cloud Classification Based on Residual Dynamic Graph Convolution and Feature Enhancement[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 37 -48 .
[5] OUYANG Shuxin, WANG Mingjun, RONG Chuitian, SUN Huabo. Anomaly Detection of Multidimensional QAR Data Based on Improved LSTM[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 49 -60 .
[6] LI Yiyang, ZENG Caibin, HUANG Zaitang. Random Attractors for Chemostat Model with Wall Attachment Driven by Fractional Brownian Motion[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 61 -68 .
[7] LI Pengbo, LI Yongxiang. Radial Symmetric Solutions of p-Laplace Equations on Exterior Domains[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 69 -75 .
[8] WU Zixian, CHENG Jun, FU Jianling, ZHOU Xinwen, XIE Jialong, NING Quan. Analysis of PI-based Event-Triggered Control Design for Semi-Markovian Power Systems[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 76 -85 .
[9] CHENG Lei, YAN Puxuan, DU Bohao, YE Si, ZOU Huahong. Thermal Stability and Dielectric Relaxation of MOF-2 Synthesized in Aqueous Phase[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 86 -95 .
[10] LIU Meiyu, ZHANG Jinyan, ZHOU Tongxi, LIAO Guangfeng, YANG Xinzhou, LU Rumei. A New C21 Steroidal Glycoside from Gymnema sylvestre and Its Hypoglycemic Activity[J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(5): 96 -104 .
Copyright © Journal of Guangxi Normal University(Natural Science Edition), All Rights Reserved.
Tel: 0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
Powered by Beijing Magtech Co. Ltd