Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (5): 143-151.doi: 10.16088/j.issn.1001-6600.2022091802

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Research on Luring Effect of 8 Metabolites on Procambarus clarkii

MO Lili1,2,3, ZHOU Zihao1,2,3, LI Dinghong3, ZENG Wenlong3, WU Zhengjun1,2,3, HUANG Jinlong1,2,3,4*, SUN Tao3*   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. Guangxi Key Laboratory of Rare and Endangered Animal Ecology (Guangxi Normal University), Guilin Guangxi 541006, China;
    3. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    4. Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), Guilin Guangxi 541006, China
  • Received:2022-09-18 Revised:2022-11-16 Published:2023-10-09

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Abstract: Pheromones can be recognized by Procambarus clarkii in water for courtship. Clarifying the effective components of P. clarkii pheromones can provide a theoretical basis for green management of aquatic invasive P. clarkii. In this research, the effects of eight standard compounds, namely 4-thiouridine, bilirubin, ethinyl estradiol, 17α-hydroxyprogesterone caproate, N-acetyl-L-leucine, GTP disodium salt, and GDP disodium salt and GMP disodium salt, on the selection behavior of P. clarkii were tested using a Y-shaped water maze, and then screened for the eliciting effect of ethinyl estradiol and 17α-hydroxyprogesterone caproate to prepare concentration assays. The results showed that the male P. clarkii showed nearly 50% selective response rate to 4-thiouridine, bilirubin, N-acetyl-L-leucine, GTP disodium salt, and GDP disodium salt and GMP disodium salt, with selection coefficients between ±0.15 and insignificant luring effects (P>0.05 ). P. clarkii showed high selection response rates to ethinyl estradiol (64.10%) and 17α-hydroxyprogesterone caproate (63.24%) with selection coefficients of 0.28 and 0.26, which showed significant sex differences. The minimum effective luring threshold of ethinyl estradiol and 17α-hydroxyprogesterone caproate to male P. clarkii were 10 and 100 μmol/L, respectively, while female P. clarkii were not sensitive to both compounds. Thus, ethinyl estradiol and 17α-hydroxyprogesterone caproate had significant luring effects on adult male P. clarkii but no significant response to female crayfish, which tentatively showed that ethinyl estradiol and 17α-hydroxyprogesterone caproate were the active components of the sex pheromone in P. clarkii.

Key words: sex pheromone, Procambarus clakii, ethinyl estradiol, 17α-hydroxyprogesterone caproate, Y-shaped maze

CLC Number:  S917.4
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