Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (5): 152-160.doi: 10.16088/j.issn.1001-6600.2022091804

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Verification of SSR Markers Significantly Associated with Height Growth Traits in Pinus massoniana

LING Jinyan1, FENG Yuanheng2,3, YANG Zhangqi2,3*, CHEN Hu2,3, TAN Jianhui2,3, GONG Guifang2,3, LUO Qunfeng2,3, FU Jun4, ZHAI Zhanggui4   

  1. 1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Guangxi Academy of Forestry, Nanning Guangxi 530002, China;
    3. Guangxi Masson Pine Engineering Technology Research Center / Key Laboratory of Mid-South Fast-growing Timber Breeding of State Forestry and Grassland Administration / Guangxi Key Laboratory of Cultivation of Excellent Timber Resources / Masson Pine Engineering Technology Research Center of State Forestry and Grassland Administration (Guangxi Academy of Forestry), Nanning Guangxi 530002, China;
    4. Guangxi Paiyangshan Forest Farm, Ningming Guangxi 532500, China
  • Received:2022-09-18 Revised:2022-10-18 Published:2023-10-09

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Abstract: Molecular marker-assisted selection breeding is an important method to obtain genes controlling target traits. In this study, four SSR markers (PCZ002, PCZ023, PCZ142 and PCZ187) associated with the growth traits of P. massoniana were selected from the second-generation full-sib families of P. massoniana. Four hybrid families were selected for each marker, with 150, 172, 157 and 163 individual materials, respectively. The association analysis of the second-generation full-sib population of P. massoniana was carried out by combining the data of tree height growth traits. The results showed that the genotypes of PCZ142-AA and PCZ142-AB were positively correlated with tree height, and the tree height was significantly higher than that of PCZ142-BB genotype, while PCZ142-BB genotype was significantly negatively correlated with tree height (P< 0.05). There was a significant positive correlation between PCZ187-BB genotype and tree height (P< 0.01), and the tree height was significantly higher than that of PCZ187-AA and PCZ187-AB genotype materials. The genotypes marked PCZ002 and PCZ023 had no significant correlation with tree height. In summary, the markers PCZ142-BB and PCZ187-BB were significantly correlated with the height growth traits of P. massoniana. The PCZ142-BB was significantly negatively correlated, and the PCZ187-BB was significantly positively correlated. These two genotype materials could become important genetic resources for the height growth traits of P. massoniana. The results of this study provide important information for the locus of height growth traits of P. massoniana in the positioning control population, and provide reference for molecular marker-assisted selection breeding of P. massoniana.

Key words: Pinus massoniana, SSR marking, tree height, association analysis, assisted breeding

CLC Number:  S791.248; Q7
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