Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (4): 189-199.doi: 10.16088/j.issn.1001-6600.2022080108

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LecRKIII.2 Gene Regulates Response of Arabidopsis thaliana to Abiotic Stress and Exogenous Hormones

XIE Daolong1,2#, ZOU Xiaoxiao1,2#, LI Meiling1,2, YOU Changqiao1,2, ZHOU Ping1,2, XIAO Wenjun1,2, GUO Xinhong1,2*   

  1. 1. Chongqing Research Institute, Hunan University, Chongqing 400039, China;
    2. Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation (Hunan University), Changsha Hunan 410082, China
  • Received:2022-08-01 Revised:2022-10-07 Online:2023-07-25 Published:2023-09-06

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Abstract: In Arabidopsis thaliana, lectin receptor-like kinases (LecRKs) contain 86 members and are widely involved in plant resistance to biotic and abiotic stresses. AtLecRKIII.2 is a member of the LecRKs family and its specific function has not been reported. Here, three-primer method and real-time quantitative PCR are used to identify homozygous deletion mutants and overexpression plants of AtLecRKIII.2 gene. Analysis of promoter cis-acting elements reveals that AtLecRKIII.2 gene has multiple regulatory elements related to stress, hormone and other responses. Tissue expression pattern analysis shows that AtLecRKIII.2 gene has the highest expression in fruit pods, followed by stems. The analysis of adversity and hormone response patterns shows that AtLecRKIII.2 gene has different degrees of response to exogenous hormones (IAA, BL, GA), PEG8000, high temperature and low temperature. The experimental results of germination rate and root length shows that after treated with NaCl or Mannitol, the seed germination rate of LecRKIII.2-OE is higher than that of the wild type, while lecrkiii.2 is lower than that of the wild type. After treated with auxin, the elongation of LecRKIII.2-OE roots is significantly inhibited and the expression level of auxin-related genes in LecRKIII.2-OE is significantly changed. The above research results indicate that the AtLecRKIII.2 gene may positively regulate the tolerance of Arabidopsis to salt stress and osmotic stress and the growth and development of roots. The above results provide reference data for further research of the physiological function of AtLecRKIII.2 gene in plant abiotic stress and hormone signal transduction.

Key words: Arabidopsis thaliana, lectin receptor-like kinases, AtLecRKIII.2, abiotic stress, hormones

CLC Number:  Q943.3
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