Journal of Guangxi Normal University(Natural Science Edition) ›› 2026, Vol. 44 ›› Issue (6): 163-174.doi: 10.16088/j.issn.1001-6600.2026011701

• Medicinal Resources Research • Previous Articles     Next Articles

Mechanism of anti-arthritic effect of ethanol extract of Sanye Qingteng on joint injury in adjuvant-induced arthritis rats based on networkpharmacology and molecular docking

Zhao Youwei1#, Hu Mengfan1,2#, Yang Yang1, Liang Liang1,3, Xiao Shundan1, Leng Jing1,3,4, Yun Chenxia1,3*   

  1. 1. School of Basic Medicine, Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China;
    2. Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China;
    3. GuangxiKey Laboratory of Characteristic Experimental Animal Model,Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China;
    4. Guangxi Key Laboratory ofTranslational Medicine for TreatingHigh Incidence Infectious Diseases with Integrative Medicine,Nanning Guangxi 530200, China
  • Received:2026-01-17 Revised:2026-04-07 Online:2026-11-05 Published:2026-11-10

Abstract: Sanye Qingteng is a characteristic Zhuang medicine in Guangxi. In folk medicine, Sanye Qingteng is often used to make wine to treat joint swelling and pain. To explore the effect and mechanism of the ethanol extract of Sanye Qingteng on joint damage in adjuvant-induced arthritis (AIA) rats, this study employed network pharmacology, molecular docking and other methods in combination with animal experiments. The results showed that the main components of the ethanol extract of Sanye Qingteng were enriched in pathways such as PI3K/Akt, and the main components, including glycocholic acid and emodin, had a strong binding ability with PI3K/Akt. After intervention with the ethanol extract of Sanye Qingteng, the joint swelling and bone damage in AIA rats were alleviated, the number of osteoclasts decreased, the expression of PI3K/Akt pathway proteins was down-regulated, the expression of bone damage-related proteins and genes such as MMP-9 was reduced, and the expression of type Ⅱ collagen was increased. The study indicated that the ethanol extract of Sanye Qingteng could alleviate joint damage in AIA rats, and the mechanism might be related to the inhibition of the PI3K/Akt pathway, the reduction of osteoclast numbers, and the down-regulation of bone damage-related proteins and genes.

Key words: Sanye Qingteng, ethanol extract, rheumatoid arthritis, bone injury, network pharmacology, molecular docking, PI3K/Akt

CLC Number:  R593.22;R285.5
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