岩黄连总生物碱提取工艺及抗氧化活性研究

doi:10.16088/j.issn.1001-6600.2021093003

Abstract

Abstract: The extraction process of total alkaloids from Corydalis saxicola Bunting was studied by single factor test and orthogonal test, using the yield of dehydrocavidine as the evaluation index. The antioxidant activity of total alkaloids of C. saxicola Bunting was evaluated by free radical scavenging method in vitro. The results showed that the optimal extraction conditions of total alkaloids were as follows: extraction temperature 80 ℃, extraction time 2.5 h, ethanol concentration 60%, solid-liquid ratio 1∶14, extraction for 3 times, under this condition the total alkaloids yield of C. saxicola Bunting was 7.34%. The antioxidant experiments showed that the crude extract of total alkaloids from C. saxicola Bunting had good scavenging effect on DPPH· and superoxide radical, and had a certain scavenging effect on ABTS⁺· and hydroxyl radical, showing in a concentration dependent manner, whick revealed that the crude extract of total alkaloids from C. saxicola Bunting are potential natural antioxidants.

Key words: Corydalis saxicola Bunting, alkaloid, extraction process, antioxidant activity

CLC Number:

R284.2

LI Ling, TANG Wendi, CHEN Shouhuan, NING Yulong, QIN Jiangke. Study on Extraction Technology and Antioxidant Activity of Total Alkaloids from Corydalis saxicola Bunting[J].Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(6): 145-153.

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Study on Extraction Technology and Antioxidant Activity of Total Alkaloids from Corydalis saxicola Bunting

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