Abstract: To establish a CZE method for simultaneous determination of gentiopicroside, loganic acid, oleanic acid, uosolic acid, isoorientin and mangiferin in the herbs of Gentiana algida Pall. 70 mmol/L borax-20% aetonitrile was used as buffer solution (pH=11). The separation was performed on an uncoated fused silica capillary (64.5 cm×50 μm,56 cm of effective length),at voltage of 22 kV. The detection wavelength was 200 nm,and the sample was injected at 50 mbar×5 s. As a result,the calibration curves of the six active ingredients showed good linearity (r>0. 999 5) in the ranges of the tested concentrations,and the average recoveries of the method were between 97.21%～98.12%. The method is simple, accurate and can be used for the quality evaluation and control of Gentiana algida Pall.

Key words: CZE, Gentiana algida Pall, active ingredient, determination