Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (4): 135-141.

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Establishment of a Screening Model for Searching Pathogenicity-related Genes in Xanthomonas oryzae pv. oryzae K74

JIANG Wei, WU De-bo, LIN Yin, YANG Wen-shan, NI Zhe, HE Yong-qiang, HUANG Sheng   

  1. College of Life Science and Technology, Guangxi University, Nanning Guangxi 530004,China
  • Received:2014-04-20 Published:2018-09-26

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Abstract: Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases in the world’s rice production. In this report, a random Tn5 transposon-mediated mutation library of Guangxi Xoo strain K74 was generated. Southern blot analysis of Tn5 insertion mutants indicated that transposon Tn5 was randomly inserted and most mutant was inserted only by a single copy Tn5. According to the leaf clip inoculation on the susceptible Nipponbare, 15 mutants were identified as reduced over 50 percents pathogenicity. Result of localized transposon Tn5 showed that mutagenesis genes included diverse functional genes: four gum cluster genes, 5 LPS cluster genes, two metB genes, two UDP-glucose dehydrogenase genes,one located downstream of hrpB, gene and one hypothetical protein gene. 14 of them are known as pathogenicity-related genes. Therefore, a screening model for searching pathogenicity-related genes in Xanthomonas oryzae pv. oryzae K74 was established successfully in this study, which laid a solid foundation for the further study of pathogenicity-related genes and clarifying their pathogenic molecular mechanism.

Key words: Xanthomonas oryzae pv. oryzae K74, Tn5 inserted mutagenesis, pathogenicity-related genes

CLC Number: 

  • Q93
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