Journal of Guangxi Normal University(Natural Science Edition) ›› 2019, Vol. 37 ›› Issue (2): 158-167.doi: 10.16088/j.issn.1001-6600.2019.02.020

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Effects of Lithology and Vegetation Type on the Soil AM Fungi Community in Karst Region

ZHANG Xiaoxiao1,2,3, WANG Miaomiao2,3, FENG Shuzhen4, QIU Husen2,3, GAI Shuangshuang5, ZHAO Lei1,2,3, HU Yajun2,3, HE Xunyang2,3*, LU Zujun1   

  1. 1.College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    2.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha Hunan 410125, China;
    3.Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang Guangxi 547100, China;
    4.College of Medicine, Guangxi University of Science and Technology, Liuzhou Guangxi 545006, China;
    5.College of Economic and Management, Guangxi Science and Technology Normal University, Liuzhou Guangxi 545004, China
  • Received:2018-03-01 Online:2019-04-25 Published:2019-04-28

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Abstract: The changes of arbuscular mycorrhizal (AM) Fungi Community Structure in karst soil under different lithological and vegetation types were detected by nested PCR and high-throughput sequencing, and the influencing factors of AM Fungi Community Structure under different lithological and vegetation types were revealed.A total of 185 OTU (Operational Taxonomic Unit) were obtained from high-throughput sequencing. These OTU could be divided into 7 families and 9 genera, of which the Glomus was the dominant genus in the study area. The results of One-way ANOVA and independent sample t-test analysis showed that there were significant differences in soil physical and chemical properties and AM fungi dominant genus between sample plots under the different lithology and vegetation type conditions. The results of Two-way ANOVA showed that the lithology and vegetation type and the interaction between them had significant influence on the diversity and community of AM fungi. The results of PCoA showed that there were significant differences in the structure of AM fungi community under the different lithology and vegetation type conditions. The results of RDA showed that soil total phosphorus, total potassium, available phosphorus, sand content and exchangeable magnesium ions affected the structure of AM fungal community. In summary, the research results showed that the lithology and vegetation type changed soil total phosphorus, total potassium, available phosphorus, sand content, exchangeable magnesium ions in soil, and thus affected the structure of AM fungi community in soil.

Key words: Karst, lithology, vegetation type, arbuscular mycorrhizal fungi, community structure

CLC Number: 

  • Q938.1
[1] 袁道先. 岩溶石漠化问题的全球视野和我国的治理对策与经验[J]. 草业科学,2008,25(9): 19-25.
[2] 王世杰. 喀斯特石漠化:中国西南最严重的生态地质环境问题[J]. 矿物岩石地球化学通报,2003,22(2):120-126.
[3] 周毅,李旋旗,赵景柱. 中国典型生态脆弱带与贫困相关性分析[J]. 北京理工大学学报,2008,28(3):260-262.
[4] ESKANDARI S, GUPPY C N, KNOX O G G, et al. Mycorrhizal symbioses of cotton grown on sodic soils: a review from an Australian perspective[J]. Pedosphere,2017,27(6):1015-1026.
[5] HEIJDEN M G A V D. Arbuscular mycorrhizal fungi as support systems for seedling establishment in grassland[J]. Ecology Letters,2004,7(4):293-303.
[6] COLLA G, ROUPHAEL Y, CARDARELLI M,et al. Alleviation of salt stress by arbuscular mycorrhizal in zucchini plants grown at low and high phosphorus concentration[J]. Biology and Fertility of Soils,2008,44(3):501-509.
[7] ZHANG L D, ZHANG J L, CHRISTIE P,et al. Effect of inoculation with the arbuscular mycorrhizal fungus Glomus intraradices on the root-knot nematode Meloidogyne incognita incucumber[J]. Journal of Plant Nutrition,2009,32(6):967-979.
[8] LI S. Different arbuscular mycorrhizal fungal species are potential determinants of plant community structure[J]. Ecology,1998,79(6):2082-2091.
[9] OEHL F, LACZKO E, BOGENRIEDER A, et al. Soil type and land use intensity determine the composition of arbuscular mycorrhizal fungal communities[J]. Soil Biology and Biochemistry, 2010,42(5):724-738.
[10] HEIJDEN M G A V D, KLIRONOMOS J N. Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity[J]. Nature, 1998,396(6706):69-72.
[11] LIANG Y M, PAN F J, HE X Y, et al. Effect of vegetation types on soil arbuscular mycorrhizal fungi and nitrogen-fixing bacterial communities in a Karst region[J]. Environmental Science and Pollution Research,2016,23(18):18482-18491.
[12] 鲍士旦. 土壤农化分析[M]. 北京:中国农业出版社,2000.
[13] WANG Y Q,SHAO M G, GAO L. Spatial variability of soil particle size distribution and fractal features in Water-Wind Erosion Crisscross Region on the Loess Plateau of China[J]. Soil Science,2010,175(12):579-585.
[14] 黄昌勇.土壤学[M]. 北京:中国农业出版社,2001.
[15] LEE J, LEE S, YOUNG J P W. Improved PCR primers for the detection and identification of arbuscular mycorrhizal fungi[J]. FEMS Microbiology Ecology,2008,65(2):339-349.
[16] SATO K, SUYAMA Y, SAITO M, et al. A new primer for discrimination of arbuscular mycorrhizal fungi with polymerase chain reaction-denature gradient gel electrophoresis[J]. Grassland Science,2005,51(2):179.
[17] 梁月明,苏以荣,何寻阳,等. 岩溶区典型灌丛植物根系丛枝菌根真菌群落结构解析[J]. 环境科学,2018,39(12): 5657-5664.
[18] 刘润进, 焦惠, 李岩,等. 丛枝菌根真菌物种多样性研究进展[J]. 应用生态学报, 2009,20(9):2301-2307.
[19] 梁月明,苏以荣,何寻阳,等. 喀斯特灌丛土壤丛枝菌根真菌群落结构及丰度的影响因子[J]. 环境科学,2017,38(11):4828-4835.
[20] TEDERSOO L, BAHRAM M, TOOTS M, et al. Towards global patterns in the diversity and community structure of ectomycorrhizal fungi[J]. Molecular Ecology,2012,21(17):4160-4170.
[21] 高程,郭良栋. 外生菌根真菌多样性的分布格局与维持机制研究进展[J]. 生物多样性,2013,21(4):488-498.
[22] 王宇涛,辛国荣,李韶山. 丛枝菌根真菌最新分类系统与物种多样性研究概况[J]. 生态学报,2013,33(3):834-843.
[23] GAUR A, ADHOLEYA A. Effects of the particle size of soilless substrates upon AM fungus inoculum production[J]. Mycorrhiza,2000,10(1):43-48.
[24] TRESEDER K K, ALLEN M F. Direct N and P limitation of arbuscular mycorrhizal fungi:a model and field test[J]. New Phytologist,2002,155(3):507-515.
[25] 张旭红,朱永官,王幼珊,等. 不同施肥处理对丛枝菌根真菌生态分布的影响[J]. 生态学报,2006,26(9):3081-3087.
[26] 李伟. 接种丛枝菌根提高柑橘对镁元素吸收及促进光合机制的研究[D]. 重庆:西南大学, 2010.
[27] 贾申,喻理飞. 喀斯特石漠化区石灰岩与白云岩土壤理化性质分析:以贵州兴义市为例[J]. 贵州科学,2010,28(3): 29-33.
[28] ANDERSON D W. The effect of parent material and soil development on nutrient cycling in temperate ecosystems[J]. Biogeochemistry,1998,5(1):71-97.
[29] 朱守谦,何纪星,魏鲁明. 茂兰喀斯特森林小生境特征研究[M].贵阳:贵州科技出版社,2003:38-48.
[30] WALDROP M P, ZAK D R, BLACKWOOD C B, et al. Resource availability controls fungal diversity across a plant diversity gradient[J]. Ecology Letters,2006,9(10):1127-1135.
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