Journal of Guangxi Normal University(Natural Science Edition) ›› 2017, Vol. 35 ›› Issue (2): 112-116.doi: 10.16088/j.issn.1001-6600.2017.02.017

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The Application of Flow Cytometry in Testing Bacteria of Raw Milk

YANG Liting1,2, HE Li2, HE Haining1, WU Qiong1,2*   

  1. 1. Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Province, Lanzhou Ganshu730000, China;
    2. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China
  • Online:2017-07-25 Published:2018-07-25

Abstract: Flow cytometry (FCM) is a rapid technique for cell-by-cell multiparameter analysis, which is often used in combination with fluorescent labelling for fast viability assessment. FCM analysss with fast and accurate protocol appeared to be workable for food bacteria detection. This article applied enzymatic treatment and membrane filtration method to digest proteins and treatment of milk by centrifugation to remove lipids. Treated milk samples were detected and enumerated by FCM. The bacteria were stained with 7-AAD and counted by fluorescent microspheres. The detection limit of 101 CFU/mL was achieved. Raw milk data showed a significant correlation (P<0.01) and a good agreement(r=0.980 2) between FCM and standard plate count methods.

Key words: flow cytometry, food detection, bacteria, milk

CLC Number: 

  • Q932
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