Journal of Guangxi Normal University(Natural Science Edition) ›› 2018, Vol. 36 ›› Issue (4): 131-138.doi: 10.16088/j.issn.1001-6600.2018.04.017

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Isolation and Identification of a BacteriumProducing Natural Blue Pigment

XIAO Miyun1,2, RUAN Chujin1,2,3, CHEN Shoukun1,2, LIU Yuhua1,2, LU Zujun1,2*   

  1. 1.College of Life Science, Guangxi Normal University, Guilin Guangxi, 541006,China;
    2. Key Laboratory of Ecological and Environmental Protection for Rare and Endangered Animalsand Plants (Guangxi Normal University), Guilin Guangxi 541006,China;
    3.Institute of Microbiology,Chinese Academy of Sciences, Beijing 100101,China
  • Received:2017-09-14 Published:2018-10-20

Abstract: In order to isolate and identify a bacterium producing natural blue pigment, surface sterilization method was applied to isolate producing natural blue pigment target strain from the root of Bovine Millettia specisoa Champ. 16S rDNA sequence of the target strain was amplified by PCR with universal primer 27F/1492R, and the phylogenetic tree was constructed by comparing and analyzing the sequence. The morphology of the bacterium was observed at the same time. The target strain was identified by the GEN III MicroStation microorganism automatic identification system. The results of the sequence alignment showed that the similarity rate of target strain and Pseudomonas aeruginosa was 99% and the phylogenetic tree showed that the target strain was on the same branch of the P. aeruginosa. Morphological identification revealed that it was rod-shaped and Gram negative, and Biolog identification showed that it was P. aeruginosa. The target strain was identified as P. aeruginosa by the combined results of molecular, morphology and Biolog identification and named as 2016NX1.

Key words: natural blue pigment, 16S rDNA, Biolog identification

CLC Number: 

  • Q939
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