基于线粒体16S rDNA基因序列沼虾属的系统发育研究

doi:10.16088/j.issn.1001-6600.2017.03.017

Abstract

Abstract: To understand the evolutionary relationship among the Macrobrachium species, mitochondrial partial 16S ribosomal DNA of M. rosenbergii was amplified and sequenced. By sequence alignment from 511 characters, 178 characters are variable and 162 characters are parsimony informative. The parameters specified under optimum model were nucleotide frequencies of η_A=0.306 3, η_C=0.065 7, η_G=0.204 6, η_T=0.423 3; reversal rates of change for [A-C]=0.042 7, [A-G]=0.248 59, [A-T]=0.028 4, [C-G]=0.032 9, [C-T]=0.629 0, [G-T]=0.018 4. The proportion of invariant sites was 0.428 7, and gamma shape distribution α=0.429 8. No substitution saturation was found as the level of transitional base substitutions was higher than the level of transversions over all genetic distances. Bayes phylogenetic tree was constructed by using Bayesian method. The out group Palaemon formed as paraphyletic to Macrobrachium with significant bootstrap value. Principally all species clustered into 3 big clades as their evolutionary relationship, in which, M. malayanum constitute a big clade, M. carcinus, M. americanum and M. vollenhovenii constituted another big clade, and the other 32 Macrobrachium constituted the third big clade. M. rosenbergii, M. japonicum, M. meridionalis, M. gracilirostre, M. jaroense, M. horstii, M. placidulum, M. lepidactyloides, M. lar, M. latimanus and M. ohione formed one cluster.

Key words: Macrobrachium, mitochondrial DNA, 16S rDNA, molecular phylogeny

CLC Number:

S917

ZHANG Xiaomin, LIN Yong, BIN Shiyu, YU Yanling,ZENG Lan, ZHONG Dandan, ZHANG Yongde. Phylogeny of Macrobrachium Species Using Mitochondrial16S Ribosomal DNA[J].Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(3): 133-140.

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Phylogeny of Macrobrachium Species Using Mitochondrial16S Ribosomal DNA

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