卵形鲳鲹Myostatin基因克隆及其在胚胎发育中的表达分析

doi:10.16088/j.issn.1001-6600.2020042602

Abstract

Abstract: Myostatin (MSTN) is a negative regulator of skeletal muscle growth,and much interest has been given to its role in animal development in recent years. In this study,MSTN gene in Trachinotus ovatus was cloned and characterized,and the expression patterns of 14 embryonic development stages of T. ovatus were studied. The results showed that 1 322 bp of MSTN gene was cloned,encoding a 376 amino acid polypeptide. The relative molecular weight was 42 694.85 u,and the theoretical isoelectric point pI was 5.52,which was a negatively charged protein. MSTN protein had no transmembrane domain and subcellular localization was mainly outside the cell,which may be secretory protein and easy to express and purify. MSTN protein had no signal peptide sequence,and 17 phosphorylation sites were predicted. A phylogenetic analysis of 24 species of fish revealed that the MSTN of T. ovatus had the highest homology with T. blochii,followed by that of S. lalandi dorsalis,all of which belonged to Carangidae,while that of Carangidae and centropomidae had the lowest genetic distance and close relationship. MSTN gene was very low or not expressed in all 13 stages of T. ovatus from Fertilized egg to Formation of eye lens stage,with no significant difference between the groups (P>0.05). However,the expression level of MSTN gene increased rapidly in the hatching stage,which was significantly higher than that in the previous 13 stages (P<0.01). The MSTN gene may play an important role after the T. ovatus hatched,but it is weak before hatching.

Key words: Trachinotus ovatus, myostatin, gene cloning, embryonic development, gene expression

CLC Number:

S965.331

LUO Honglin, FENG Pengfei, YU Yanling, XIAO Rui, PAN Chuanyan, SONG Manling, ZHANG Yongde. Molecular Cloning of the Myostatin Gene and Its Expression During Embryo Development of Trachinotus ovatus[J].Journal of Guangxi Normal University(Natural Science Edition), 2021, 39(1): 136-147.

References

[1] 熊向英,王贤丰,彭银辉,等. 健康和患病卵形鲳鲹肠道菌群结构的差异[J]. 水产学报,2019,43(5): 1317-1325.DOI: 10.11964/jfc.20180411227.
[2] 胡海滨,钱雪桥,解绶启,等. 中成鱼阶段卵形鲳鲹对饲料蛋白质和脂肪的适宜需求量[J]. 上海海洋大学学报,2019,28(4): 566-576. DOI: 10.12024/jsou.20181202459.
[3] 易新文,陈瑞爱,徐家华. 鸡肉粉替代鱼粉对卵形鲳鲹生长、饲料利用和抗氧化力的影响[J]. 中国海洋大学学报(自然科学版),2019,49(12): 17-24. DOI: 10.16441/j.cnki.hdxb.20180203.
[4] TAN X H,SUN Z Z,ZHOU C P,et al. Effects of dietary dandelion extract on intestinal morphology,antioxidant status,immune function and physical barrier function of juvenile golden pompano Trachinotus ovatus[J]. Fish and Shellfish Immunology,2018,73: 197-206. DOI: 10.1016/j.fsi.2017.12.020.
[5] THOMAS M,LANGLEY B,BERRY C,et al. Myostatin,a negative regulator of muscle growth,functions by inhibiting myoblast proliferation[J]. Journal of Biological Chemistry,2000,275(51): 40235-40243.DOI: 10.1074/jbc.M004356200.
[6] McPHERRON A C,LAWLER A M,LEE S J. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member[J]. Nature,1997,387(6628): 83-90. DOI: 10.1038/387083a0.
[7] MOSHER D S,QUIGNON P,BUSTAMANTE C D,et al. A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs[J]. PLoS Genetics,2007,3(5): e79. DOI: 10.1371/journal.pgen.0030079.
[8] MEDEIROS E F,PHELPS M P,FUENTES F D,et al. Overexpression of follistatin in trout stimulates increased muscling[J]. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology,2009,297(1):R235-R242.
[9] LIN J,ARNOLD H B,DELLA-FERA M A,et al. Myostatin knockout in mice increases myogenesis and decreases adipogenesis[J]. Biochemical and Biophysical Research Communications,2002,291(3): 701-706.
[10] LI Q,LI Y G,HUANG X D,et al. Molecular cloning and single nucleotide polymorphisms identification of myostatin cDNA in the pearl oyster,Pinctada fucata[J]. Journal of the World Aquaculture Society,2014,45(6): 638-651.
[11] CHIANG Y A,KINOSHITA M,MAEKAWA S,et al. TALENs: mediated gene disruption of myostatin produces a larger phenotype of medaka with an apparently compromised immune system[J]. Fish and Shellfish Immunology,2016,48: 212-220.
[12] GAO F,SUN B X,XING S Y,et al. The effect of leader peptide mutations on the biological function of bovine myostatin gene[J]. Gene,2014,540(2): 171-177.
[13] HILL E W,GU J J,EIVERS S S,et al. A sequence polymorphism in MSTN predicts sprinting ability and racing stamina in thoroughbred horses[J]. PLoS ONE,2010,5(1): e8645.
[14] 李景芬,采克俊,曹访,等. 反向、巢式、降落PCR技术克隆奥尼罗非鱼MSTN基因3'侧翼序列[J]. 江苏农业科学,2014,42(2): 28-30.
[15] 李兴美,范巍,张彬,等. 鲤鱼肌肉生长抑制素基因(MSTN)的克隆及其组织表达特征[J]. 水生生物学报,2007,31(5): 643-648.
[16] 钟其旺. 几种鲆鲽鱼类Myostatin基因的克隆及结构和表达分析[D]. 青岛: 中国海洋大学,2007.
[17] 李盈,额尔敦木图,刘图雅,等. 肌肉生长抑制素基因在不同动物中的研究进展[J]. 中国畜牧兽医,2013,40(8): 141-145.
[18] 彭扣,赵浩斌,彭英,等. 普通鲤与建鲤MSTN基因比较分析[J]. 南昌大学学报(理科版),2013,37(2): 191-197.
[19] 区又君,李加儿. 卵形鲳鲹的早期胚胎发育[J]. 中国水产科学,2005,12(6): 786-788.
[20] 张猛,陈勇,沈玉帮,等. 草鱼 MSTN-1 基因多态性及与早期生长性状和肌肉成分关联分析[J]. 水产学报,2016,40(4): 618-625.
[21] 张冬杰,刘娣,张旭,等. 利用CRISPR-Cas9系统定点突变猪MSTN基因的研究[J]. 畜牧兽医学报,2016,47(1): 207-212.
[22] 王发新. 兰州鲇GH和MSTN基因多态性及生长相关性研究[D]. 兰州: 甘肃农业大学,2016.
[23] KONDRASHOV F A, ROGOZIN I B, WOLF Y I, et al. Selection in the evolution of gene duplications[J]. Genome Biology,2002,3(2): research0008.1.
[24] ZHANG S Y,LI Y,SHAO J J,et al. Functional identification and characterization of IpMSTNa,a novel orthologous myostatin (MSTN) gene in channel catfish Ictalurus punctatus[J]. International Journal of Biological Macromolecules,2020,152: 1-10.
[25] 俞兆曦. 兰州鲇生长性状相关基因的分离、序列特征及组织特异性表达分析[D]. 兰州: 甘肃农业大学,2016.
[26] 易恒洁,李辉,杨胜林,等. 三穗鸭MyoG基因多态性与屠宰性状的相关性研究[J]. 中国畜牧杂志,2016,52(9): 5-10.
[27] 李婷. 黑鲷MSTNⅡ基因分子特征[D]. 宁波:宁波大学,2009.
[28] 孙冉冉. 黄条鰤肌肉生长因子的克隆及其表达规律的研究[D]. 大连: 大连海洋大学,2019.
[29] BETANCUR-R R,WILEY E O,ARRATIA G,et al. Phylogenetic classification of bony fishes[J]. BMC Evolutionary Biology,2017,17: 162.
[30] HELTERLINE D L I,GARIKIPATI D,STENKAMP D L,et al. Embryonic and tissue-specific regulation of myostatin-1 and -2 gene expression in zebrafish[J]. General and Comparative Endocrinology,2007,151(1): 90-97.
[31] VIANELLO S,BRAZZODURO L, DALLA VALLE L,et al. Myostatin expression during development and chronic stress in zebrafish (Danio rerio)[J]. Journal of Endocrinology,2003,176(1): 47-59.
[32] XU C,WU G,ZOHAR Y,et al. Analysis of myostatin gene structure,expression and function in zebrafish[J]. Journal of Experimental Biology,2003,206(22): 4067-4079.

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Molecular Cloning of the Myostatin Gene and Its Expression During Embryo Development of Trachinotus ovatus

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