Journal of Guangxi Normal University(Natural Science Edition) ›› 2016, Vol. 34 ›› Issue (1): 144-149.doi: 10.16088/j.issn.1001-6600.2016.01.023

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Effect of Starvating and Refeeding on Relative Expression of FSRP-1,FSRP-3 and PepT1 Gene in Mandarin Fish (Siniperca chuatsi)

WANG Jianhua1,2, LI Yulong2, CHEN Dunxue1, ZHU Xin2, LIU Zhixing2, ZHANG Jianshe2, CHU Wuying2, BIN Shiyu1   

  1. 1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541006,China; 2. Department of Bioengineering and Environmental Science, Changsha University, Changsha Hunan 410003, China
  • Received:2015-07-25 Published:2018-09-14

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Abstract: To investigate postprandial changes in transcript abundance following a single satiating meal in mandarin fish (Siniperca chuatsi) going on a fast for 1 week. The expression of FSRP-1 and FSRP-3 gene in fast myotomal muscle, as well as the PepT1 gene in small intestine were determined using quantitative real-time PCR at the following postprandial time points: 0, 1, 3, 6, 12, 24, 48 and 96 h. The results indicated that: FSRP-1 transcripts were sharply up-regulated within 12 h of refeeding (P<0.05), the transcripts were maintained in the high level from 12 h to 48 h, then it was significant down-regulation in response to feeding; The FSRP-3 gene expression was increased significantly (P<0.05) in 1 h postprandially, and reached peak expression levels at 12 h postprandially, and decreased significantly after 48 h postprandially (P<0.05). The expression level of FSRP-3 at 12 h was 5 times higher compared with which at 1 h; The PepT1 gene had decreased expression in 6 h after the meal administration significantly (P<0.05), and reached maximal expression levels at 48 h postprandially. FSRP-1, FSRP-3 and PepT1 were absolutely necessary, associated with growth, which were greatly influence by starvation and refeeding.

Key words: Siniperca chuatsi, starvation and refeeding, FSRP-1 and FSRP-3, PepT1, gene expression

CLC Number: 

  • S965.127
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