Journal of Guangxi Normal University(Natural Science Edition) ›› 2016, Vol. 34 ›› Issue (1): 144-149.doi: 10.16088/j.issn.1001-6600.2016.01.023

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Effect of Starvating and Refeeding on Relative Expression of FSRP-1,FSRP-3 and PepT1 Gene in Mandarin Fish (Siniperca chuatsi)

WANG Jianhua1,2, LI Yulong2, CHEN Dunxue1, ZHU Xin2, LIU Zhixing2, ZHANG Jianshe2, CHU Wuying2, BIN Shiyu1   

  1. 1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541006,China; 2. Department of Bioengineering and Environmental Science, Changsha University, Changsha Hunan 410003, China
  • Received:2015-07-25 Published:2018-09-14

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Abstract: To investigate postprandial changes in transcript abundance following a single satiating meal in mandarin fish (Siniperca chuatsi) going on a fast for 1 week. The expression of FSRP-1 and FSRP-3 gene in fast myotomal muscle, as well as the PepT1 gene in small intestine were determined using quantitative real-time PCR at the following postprandial time points: 0, 1, 3, 6, 12, 24, 48 and 96 h. The results indicated that: FSRP-1 transcripts were sharply up-regulated within 12 h of refeeding (P<0.05), the transcripts were maintained in the high level from 12 h to 48 h, then it was significant down-regulation in response to feeding; The FSRP-3 gene expression was increased significantly (P<0.05) in 1 h postprandially, and reached peak expression levels at 12 h postprandially, and decreased significantly after 48 h postprandially (P<0.05). The expression level of FSRP-3 at 12 h was 5 times higher compared with which at 1 h; The PepT1 gene had decreased expression in 6 h after the meal administration significantly (P<0.05), and reached maximal expression levels at 48 h postprandially. FSRP-1, FSRP-3 and PepT1 were absolutely necessary, associated with growth, which were greatly influence by starvation and refeeding.

Key words: Siniperca chuatsi, starvation and refeeding, FSRP-1 and FSRP-3, PepT1, gene expression

CLC Number: 

  • S965.127
[1] CHU Wuying, LI Yulong, WU Ping, et al. Characterization and expression analysis of myogenin gene in white muscle of Chinese Mandarin Fish, Siniperca chuatsi[J]. J Proteomics Bioinform, 2014, 7:3, 71-76.
[2] ZHU Xin, LI Yulong, CHEN Dunxue, et al. Selection of reference genes for microRNA quantitative expression analysis in Chinese perch, Siniperca chuatsi[J]. Int J Mol Sci, 2015, 16(4):8310-8323.
[3] MEHNER T, WIESER W. Energeties and metabolie correlates of starvation in juvenile Pereh (Perca fluviatilis)[J]. Fish Biology, 1994, 45:325-333.
[4] DOBSON S H, HOLMES R M. Compensatory growth in the rainbow trout (Salmo gairdneri) Richardson[J]. Fish Biol, 1984, 25:649-656.
[5] ZHU Xin, CHEN Dun, HU Yi, et al. The microRNA signature in response to nutrient restriction and refeeding in skeletal muscle of Chinese perch (Siniperca chuatsi)[J]. Mar Biotechnol (NY), 2015, 17(2):180-189.
[6] CHEN Lin, WU Ping, GUO Xinhong, et al. miR-143: a novel regulator of MyoD expression in fast and slow muscles of Siniperca chuatsi[J]. Curr Mol Med, 2014, 14(3):370-375.
[7] 王海芳, 刘霞, 徐亚娟. 饥饿再投喂对“甘肃金鳟”幼体生长和肌肉组分的影响[J]. 甘肃农业大学学报, 2014(5):37-42.
[8] 陈丽萍. 饥饿再投喂对团头鲂幼鱼生肌调节因子表达和肌肉特性的影响[D]. 武汉:华中农业大学, 2013.
[9] KOTA J, HANDY C R, HAIDET A M, et al. Follistatin gene delivery enhances muscle growth and strength in nonhuman primates[J]. Sci Transl Med, 2009, 1(6):1-8.
[10] 黎航航. 草鱼PepT1与PepT2基因全长cDNA克隆和PepT2组织表达研究[D]. 长沙:湖南农业大学, 2012.
[11] LIVAK K J, THOMAS D S. Analysis of relative gene expression data using real time quantitative PCR and the 2-ΔΔCt method[J]. Methods, 2001, 25:402-408.
[12] 覃川杰, 邵婷, 杨洁萍, 等. 饥饿胁迫对瓦氏黄颡鱼脂肪代谢的影响[J]. 水生生物学报, 2015(1):58-65.
[13] MIYABE M, OHASHI K, SHIBATA R, et al. Muscle-derived follistatin-like 1 functions to reduce neointimal formation after vascular injury[J]. Cardiovasc Res, 2014, 103(1):111-120.
[14] 钱天梅, 陆仁飞, 周晓芳, 等. 多疣壁虎Fstl1基因表达及其在断尾损伤中的变化[J]. 南通大学学报(医学版), 2010(1):10-13.
[15] 申华, 王绿娅, 刘宇扬, 等. 诱导血管平滑肌细胞FRP基因表达的机制研究[J]. 中华中医药学刊, 2009(2):286-289.
[16] OLDKNOW K J, SEEBACHER J, GOSWAMI T, et al. Follistatin-like 3 (FSTL3) mediated silencing of transforming growth factor β (TGF β) signaling is essential for testicular aging and regulating testis size[J]. Endocrinology, 2013, 154(3):1310-1320.
[17] CHU Wuying, LIU Lusha, LI Yulong, et al. Systematic identification and differential expression profiling of microRNAs from white and red muscles of Siniperca chuatsi[J]. Curr Mol Med, 2013, 13(8):1397-1407.
[18] 王开卓. 翘嘴鳜Smyd1和Myostatin基因的克隆及其表达分析[D]. 桂林:广西师范大学, 2014.
[19] TEROVA G, CORà S, VERRI T, et al. Impact of feed availability on PepT1 mRNA expression levels in sea bass (Dicentrarchus labrax)[J]. Aquaculture, 2009, 294(3/4):288-299.
[20] YANIV H, SHEENAN H, ZEHAVA U. Expression of brush border enzymes and transporters in the intestine of European sea bass (Dicentrarchus labrax) following food deprivation[J]. Aquaculture, 2009, 290(1/2):110-115.
[21] KOVEN W, SCHULTE P. The effect of fasting and refeeding on mRNA expression of PepT1 and gastrointestinal hormones regulating digestion and food intake in zebrafish (Danio rerio)[J]. Fish Physiol Biochem, 2012, 38(6):1565-1575.
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