广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (6): 179-191.doi: 10.16088/j.issn.1001-6600.2023031303

• • 上一篇    下一篇

凋落物及根系输入变化对喀斯特地区檵木土壤养分和胞外酶的影响

刘佩雯1,2, 覃云斌1,2,3, 莫慧婷1,2, 周珍辉1,2, 蒙伟明1,2, 黄启祥1,2, 马姜明1,2,3*   

  1. 1.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西 桂林 541006;
    2.广西漓江流域景观资源保育与可持续利用重点实验室(广西师范大学), 广西 桂林 541006;
    3.广西师范大学 可持续发展创新研究院, 广西 桂林 541006
  • 收稿日期:2023-03-13 修回日期:2023-04-24 发布日期:2023-12-04
  • 通讯作者: 马姜明(1976—), 男, 江西永新人, 广西师范大学教授, 博士。 E-mail: mjming03@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金(U21A2007, 32260387); 广西重点研发计划(桂科AB21220057, 桂科AB21196065); 广西创新驱动发展专项(桂科AA20161002)

Effects of Litter and Root Input and Removal on Soil Nutrient, Enzyme Activity, and Stoichiometry in Karst of Loropetalum chinense

LIU Peiwen1,2, QIN Yunbin1,2,3, MO Huiting1,2, ZHOU Zhenhui1,2, MENG Weiming1,2, HUANG Qixiang1,2, MA Jiangming1,2,3*   

  1. 1. Key Laboratory of Ecology and Environmental Protection of Rare and Endangered Animals and Plants, Ministry of Education (Guangxi Normal University), Guilin Guangxi 541006, China;
    2. Guangxi Key Laboratory for Conservation and Sustainable Utilization of Landscape Resources in Lijiang River Basin (Guangxi Normal University), Guilin Guangxi 541006, China;
    3. Institute of Sustainable Development and Innovation, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2023-03-13 Revised:2023-04-24 Published:2023-12-04

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摘要： 凋落物输入变化能改变土壤的理化性质,显著影响土壤碳氮磷养分含量和胞外酶活性,引起胞外酶活性化学计量特征的变化。为进一步明确喀斯特地区凋落物输入变化对土壤养分含量、胞外酶活性及其化学计量的影响,并探究胞外酶与环境因子间的生态响应规律,本文以喀斯特地区檵木老龄林土壤为研究对象,布设凋落物添加和去除试验,通过对土壤养分及胞外酶活性的测定,运用生态化学计量学理论与方法,系统研究土壤中6种胞外水解酶(β-1,4-葡糖苷酶(BG)、 β-1,4-木糖苷酶(BX)、 纤维素水解酶(CBH)、 β-1,4-乙酰氨基葡萄糖苷酶(NAG)、 亮氨酸氨基肽酶(LAP)、 酸性磷酸酶(AP))和2种胞外氧化酶(过氧化氢酶(CAT)、 多酚氧化酶(PPO))活性及其化学计量的影响, 并分析它们与土壤环境因子之间的相关性。 结果表明: 1)与对照(CK)相比,土壤SOC、TN含量在凋落物添加下具有显著性变化,去除凋落物根系(ABR)和双倍凋落物添加(AD)处理的土壤C∶N值显著增大;2)过氧化氢酶活性CK处理下最高, 且与其他处理具有显著性差异; 多酚氧化酶活性ABR处理最高, 且与AD和BR处理差异性显著; 土壤胞外酶活性的矢量角度小于45°, 表明檵木老龄林土壤主要受氮元素限制; 3)RDA分析结果表明,土壤胞外酶BX、CBH、NAG含量及胞外酶C∶N和N∶P与土壤速效磷和氨态氮之间存在显著正相关;土壤胞外酶LAP、AP、BG、CAT、PPO含量及胞外酶C∶P与土壤速效钾含量正相关,与总磷和硝态氮含量负相关。

关键词: 喀斯特地区, 凋落物输入, 土壤养分, 土壤胞外酶, 化学计量比

Abstract: The changes in soil physicochemical properties caused by changes in litter input can significantly affect the content of soil carbon, nitrogen, and phosphorus nutrients, as well as changes in soil extracellular enzyme activity and stoichiometry characteristics. To further clarify the impact of changes in litter input on soil nutrient content, extracellular enzyme activity, and stoichiometry in karst areas, and to explore the ecological response patterns between extracellular enzymes and environmental factors, in this study, the soil of Loropetalum chinense aged forest in karst area was taken as the research object, and litter addition and removal experiments were arranged. Through the determination of soil nutrients and extracellular enzyme activities, the theory and methods of Ecological stoichiometry were used to systematically study six extracellular hydrolases in the soil(β-1,4-glucosidase (BG), β-1,4-Xylosidase (BX), cellulose hydrolase (CBH), β-1,4-acetylglucosaminidase (NAG), leucine aminopeptidase (LAP), acid phosphatase (AP) and two extracellular enzymes (catalase (CAT) and polyphenol oxidase (PPO)), as well as their stoichiometric effects, were studied, and their correlation with soil environmental factors were analyzed. The results showed that: (1) Compared with the control (CK), the content of soil SOC and TN showed significant changes under the addition of litter; And the C∶N value significantly increased in the treatment of removing the root system of litter (ABR) and adding double litter (AD); (2) The activity of catalase was the highest under the control (CK) treatment, and there was a significant difference compared with other treatments; The activity of polyphenol oxidase was the highest in the treatment of removing litter roots (ABR), and there was a significant difference compared with the treatment of double litter addition (AD) and root removal (BR); In addition, the vector angle of soil enzyme activity was less than 45°, indicating that the soil of the aged Loropetalum chinense forest was mainly limited by nitrogen elements; (3) The results of RDA analysis showed that there was a significant positive correlation between soil extracellular enzymes BX, CBH, NAG content, enzyme C∶N, and enzyme N∶P, and soil available phosphorus and ammonia nitrogen; The content of soil extracellular enzymes LAP, AP, BG, CAT, PPO, and enzyme C∶P were positively correlated with soil available potassium content, but negatively correlated with total phosphorus and nitrate nitrogen content.

Key words: karst area, litter input, soil nutrient, soil extracellular enzyme, stoichiometryic ratio

中图分类号:  S154, S714

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