漓江3种淡水螺类肠道微生物研究

doi:10.16088/j.issn.1001-6600.2024112502

摘要/Abstract

摘要： 格氏短沟蜷、三带田螺和绘石田螺均是重要的淡水经济螺类,在水生态恢复中发挥着重要作用。水生动物的肠道微生物在食物消化和营养吸收中起至关重要的作用。本研究采用16S rDNA高通量测序方法,对漓江3种淡水螺肠道微生物组成和功能进行比较。结果表明,在门水平上,格氏短沟蜷、三带田螺和绘石田螺的菌群组成中共有的优势菌为变形菌门Proteobacteria、厚壁菌门Firmicutes、拟杆菌门Bacteroidetes和酸杆菌门Actinobacteria。其中,变形菌门在3组中都是丰度最大的菌门。在属水平上,共有的优势菌有气单胞菌属Aeromonas、不动杆菌属Acinetobacter和假单胞菌属Pseudomonas。在群落多样性方面,格氏短沟蜷与三带田螺之间无显著差异(P>0.05),而绘石田螺与格氏短沟蜷和三带田螺之间存在极显著差异(P<0.01)。基于PICRUSt分析预测格氏短沟蜷、三带田螺和绘石田螺肠道菌群的功能,3种螺的肠道微生物主要参与新陈代谢过程,包括氨基酸代谢、辅酶和维生素代谢、异生物降解与代谢、萜类和多酮类化合物代谢、脂质代谢以及其他氨基酸代谢等。

关键词: 肠道微生物, 16S rDNA, 高通量测序, 淡水螺, 功能预测

Abstract: Semisulcospira gredleri, Viviparus tricinctus and Sinotaia limnophila are all important economic freshwater snail species, which play significant ecological roles in the restoration of water ecology. The gut microbes of aquatic animals play a crucial role in food digestion and nutrient absorption. Therefore, in this study, 16S rDNA high-throughput sequencing was use to compare the composition and function of gut microbiota in three species of freshwater snails from the Lijiang River. The results showed that at the phylum level, the common dominant microorganisms in the three groups were Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria. Proteobacteria was the most abundant bacteria in both groups. At the genus level, the common dominant bacteria were Aeromonas, Acinetobacter, Pseudomonas. In terms of community diversity, there was no significant difference between Semisulcospira gredleri and Viviparus tricinctus (P>0.05), while there was a highly significant difference between Sinotaia limnophila and both Semisulcospira gredleri and Viviparus tricinctus (P<0.01). Based on PICRUSt analysis, the intestinal microbiota of three species of snails was predicted to be mainly involved in metabolic processes, including amino acid metabolism, metabolism of cofactors and vitamins, xenobiotics biodegradation and metabolism, metabolism of terpenoids and polyketides, lipid metabolism, metabolism of other amino acids.

Key words: intestinal flora, 16S rDNA, high-throughput sequencing, freshwater snail, functional prediction

中图分类号: S917.4

庞丽珍, 杜丽娜, 王波. 漓江3种淡水螺类肠道微生物研究[J]. 广西师范大学学报（自然科学版）, 2025, 43(5): 207-217.

PANG Lizhen, DU Lina, WANG Bo. Study on Intestinal Microorganisms of Three Freshwater Snails in Lijiang River[J]. Journal of Guangxi Normal University(Natural Science Edition), 2025, 43(5): 207-217.

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