广西师范大学学报（自然科学版） ›› 2020, Vol. 38 ›› Issue (1): 93-101.doi: 10.16088/j.issn.1001-6600.2020.01.012

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甲泼尼龙对单侧输尿管梗阻诱导的肾纤维化的影响

龙靖娴1, 潘毓敏2, 潘国律2, 黄容诚2, 彭艳2*   

  1. 1.广西师范大学生命科学学院,广西桂林541006;
    2.广西师范大学化学与药学学院,广西桂林541004
  • 收稿日期:2018-11-26 出版日期:2020-01-25 发布日期:2020-01-15
  • 通讯作者: 彭艳(1968—),女,辽宁锦州人,广西师范大学研究员。E-mail:pengyan630@aliyun.com
  • 基金资助:
    国家自然科学基金(81673473)

Effect of Methylprednisolone on Unilateral Ureteral Obstruction-induced Renal Fibrosis

LONG Jingxian1, PAN Yumin2, PAN Guolü2, HUANG Rongcheng2, PENG Yan2*   

  1. 1. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2018-11-26 Online:2020-01-25 Published:2020-01-15

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摘要： 为探讨甲泼尼龙在小鼠肾纤维化中的作用,本文通过单侧输尿管梗阻(unilateral ureteral obstruction, UUO)实验建立肾纤维化模型。实验将25只雄性Balb/c小鼠随机分为假手术组、模型组、低剂量组、中剂量组、高剂量组。手术后第2天给药,14 d后,使用HE染色和Masson染色检测各组小鼠肾脏病理变化情况。Western blotting和IHC染色检测各组梗阻侧肾脏中Col-1、Col3A1、FN、α-SMA的表达情况以及TGF-β/Smad和NF-κB信号通路中蛋白的表达情况。结果显示UUO会导致小鼠肾间质纤维化,甲泼尼龙能显著减弱UUO小鼠肾间质细胞外基质Col-1、Col3A1、FN、α-SMA的生成,下调TGF-β/Smad和NF-κB信号通路中蛋白的表达。由此可知,甲泼尼龙对小鼠肾纤维化有明显的改善作用,它通过抑制TGF-β/Smad和NF-κB信号通路来减轻UUO诱导的肾纤维化。

关键词: 甲泼尼龙, UUO, TGF-β/Smad, NF-κB

Abstract: To investigate the role of methylprednisolone in renal fibrosis in mice,A renal fibrosis model was established by Unilateral Ureteral Obstruction (UUO) experiment. Twenty-five male Balb/c mice were randomly divided into sham operation group, model group, low dose group, medium dose group and high dose group. The drug was administered on the second day after the surgery, and 14 days later, renal pathological changes were detected by HE staining and Masson staining. Western blotting and IHC staining were used to detect the expression of Col-1, Col3A1, FN and α-SMA in the obstructed kidneys of each group and to detect the expression of TGF-β/Smad and NF-κB signaling pathways. The results showed that UUO can cause renal interstitial fibrosis. Methylprednisolone significantly attenuated the production of extracellular matrix Col-1, Col3A1, FN and α-SMA in UUO mice, and down-regulated expression of the protein in TGF-β/Smad and NF-κB signaling pathways. It can be seen that methylprednisolone has a significant improvement in renal fibrosis in mice, which reduces UUO-induced renal fibrosis by inhibiting TGF-β/Smad and NF-κB signaling pathways.

Key words: Methylprednisolone sodium succinate, UUO, TGF-β/Smad, NF-κB

中图分类号: 

  • R692
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