广西师范大学学报（自然科学版） ›› 2019, Vol. 37 ›› Issue (4): 103-110.doi: 10.16088/j.issn.1001-6600.2019.04.013

• • 上一篇    下一篇

白芷水提液镇痛作用及其机理研究

郭珺, 陈丹, 朱婵, 唐宗湘*   

  1. 南京中医药大学医学与生命科学学院,江苏南京210023
  • 收稿日期:2019-02-27 出版日期:2019-10-25 发布日期:2019-11-28
  • 通讯作者: 唐宗湘(1964—),男,湖南会同人,南京中医药大学教授,博导。E-mail:zongxiangtang1@163.com
  • 基金资助:
    国家自然科学基金面上项目(31471007,31771163)

Analgesic Effect and Analgesic Mechanism of Angelica dahurica Extracts

GUO Jun, CHEN Dan, ZHU Chan, TANG Zongxiang*   

  1. School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing Jiangsu 210023, China
  • Received:2019-02-27 Online:2019-10-25 Published:2019-11-28

PDF (PC)

195

摘要： 采用浸尾实验、热辐射实验、辣椒素和福尔马林脚掌致痛实验以及Von-Frey动物行为学实验,探究白芷水提液(ADE)的镇痛作用,并通过原代细胞培养和钙成像技术探讨白芷水提液的镇痛作用机理。结果发现,白芷水提液可有效抑制热浴浸尾和热辐射引起的伤害性热痛以及由辣椒素或福尔马林诱导的疼痛。然而,在Trpv1基因敲除小鼠中,白芷水提液不能缓解因热刺激或辣椒素引起的小鼠疼痛行为。此外,在小鼠背根神经节神经元中,白芷水提液能显著抑制由辣椒素刺激引起的钙离子内流。可见白芷水提液具有显著的镇痛作用,其镇痛作用与抑制TRPV1通道功能紧密相关。

关键词: 白芷, 镇痛, TRPV1 , 热痛, 急性痛

Abstract: The analgesic effect of ADE was evaluated by a series of pain models, in which the pain behaviours were induced by tail immersion, thermal radiation, capsaicin and formalin tests, and Von-Frey mechanical stimulation, respectively. The primary cell culture and calcium imaging technique were then employed to investigate the functional mechanism of ADE in neurons from dorsal root ganglion (DRG). The results showed that the ADE substantially attenuated the acute pain induced by thermal, capsaicin, and formalin in mice. However, ADE did not suppress the thermal-or capsaicin-induced acute pain in Trpv1-/- mice. Moreover, ADE significantly inhibited the intracellular influx of Ca2+ induced by capsaicin in the TRPV1-positive DRG neurons. So these findings suggested that ADE exhibits an analgesic effect in pain models by reducing the activity of TRPV1.

Key words: Angelica dahurica, analgesic effect, TRPV1, thermal pain, acute pain

中图分类号: 

  • R282.71
[1] 张愚. 楚辞译注[M]. 济南: 山东教育出版社, 1986: 4-5.
[2] 梁·陶弘景. 本草经集注(尚志钧等辑校)[M]. 北京: 人民卫生出版社, 1994: 294.
[3] 崔秋兵,张艺,兰莎. 白芷镇痛作用物质基础研究[J]. 中国实验方剂学杂志, 2010, 16(12): 102-104.
[4] CHAPLAN S R, BACH F W, POGREL J W, et al. Quantitative assessment of tactile allodynia in the rat paw[J]. Journal of Neuroscience Methods, 1994, 53(1): 55-63.
[5] 彭小莉,高喜玲,陈军,等. 白芷水煎剂和冻干粉对大鼠外周组织损伤所致的持续性自发痛、热和机械痛敏作用的比较研究[J]. 中国中西医结合杂志, 2004(增刊1): 243-247.
[6] CATERINA M J, SCHUMACHER M A, TOMINAGA M, et al. The capsaicin receptor: a heat-activated ion channel in the pain pathway[J]. Nature, 1997, 389(6653): 816-824.
[7] TJOLSEN A, BERGE O G, HUNSKAAR S, et al. The formalin test: an evaluation of the method[J]. Pain, 1992, 51(1): 5-17.
[8] TOMINAGA M, CATERINA M J, MALMBERG A B, et al. The cloned capsaicin receptor integrates multiple pain-producing stimuli[J]. Neuron,1998, 21(3): 531-543.
[9] CATERINA M J, LEFFLER A, MALMBERG A B, et al. Impaired nociception and pain sensation in mice lacking the capsaicin receptor[J]. Science, 2000, 288(5464): 306-313.
[10]周志梅,郭世民,何静,等. 镇痛中药的研究进展[J]. 云南中医中药杂志, 2007(4): 52-54.
[11]SARKER S D, NAHAR L. Natural medicine: the genus Angelica[J]. Current Medicinal Chemistry, 2004, 11(11): 1479-1500.
[12]YANG W T, KE C Y, WU W T, et al. Effects of Angelica dahurica and rheum officinale extracts on excisional wound healing in rats[J]. Evidence-based Complementary and Alternative Medicine, 2017, 2017(21):1-8.
[13]LEE M Y, SEO C S, LEE J A, et al. Anti-asthmatic effects of Angelica dahurica against ovalbumin-induced airway inflammation via upregulation of heme oxygenase-1[J]. Food and Chemical Toxicology: an International Journal Published for the British Industrial Biological Research Association,2011, 49(4): 829-837.
[14]KIM Y K, KIM Y S, RYU S Y. Antiproliferative effect of furanocoumarins from the root of Angelica dahurica on cultured human tumor cell lines[J]. Phytotherapy Research: PTR,2007, 21(3): 288-290.
[15]WANG G, CHEN C, TSAI T, et al. Evaluation of tyrosinase inhibitory and antioxidant activities of Angelica dahurica root extracts for four different probiotic bacteria fermentations[J]. Journal of Bioscience and Bioengineering,2017, 123(6): 679-684.
[16]WANG C, SUN J, LI H, et al. In vivo anti-inflammatory activities of the essential oil from Radix Angelicae dahuricae[J]. Journal of Natural Medicines, 2016, 70(3): 563-570.
[17]MICKLE A D, SHEPHERD A J, MOHAPATRA D P. Sensory TRP channels: the key transducers of nociception and pain[J]. Progress in Molecular Biology and Translational Science,2015, 131: 73-118.
[18]LIAO H Y, HSIEH C L, HUANG C P, et al. Electroacupuncture attenuates CFA-induced inflammatory pain by suppressing Nav1.8 through S100B, TRPV1, Opioid, and Adenosine Pathways in Mice[J]. Scientific Reports,2017, 7: 42531.
[19]CHEN X, SUN W, GIANARIS N G, et al. Furanocoumarins are a novel class of modulators for the transient receptor potential vanilloid type 1 (TRPV1) channel[J]. The Journal of Biological Chemistry,2014, 289(14): 9600-9610.
[20]王梦月,贾敏如,马逾英,等. 白芷中四种线型呋喃香豆素类成分药理作用研究[J]. 天然产物研究与开发,2010, 22(3): 485-489.
[1] 邓志勇, 骆海玉, 陈超英, 邓业成, 孙文斌, 梁芳铭. 木荷树皮乙醇提取物抗炎镇痛作用研究[J]. 广西师范大学学报（自然科学版）, 2019, 37(1): 181-186.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 孟春梅, 陆世银, 梁永红, 莫肖敏, 李卫东, 黄远洁, 成晓静, 苏志恒, 郑华. 岩黄连总碱诱导肝星状细胞凋亡和自噬的电镜实验研究[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 76 -79 .
[2] 李钰慧, 陈泽柠, 黄中豪, 周岐海. 广西弄岗熊猴的雨季活动时间分配[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 80 -86 .
[3] 庄枫红, 马姜明, 张雅君, 苏静, 于方明. 中华水韭对不同光照条件的生理生态响应[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 93 -100 .
[4] 韦宏金, 周喜乐, 金冬梅, 严岳鸿. 湖南蕨类植物增补[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 101 -106 .
[5] 包金萍, 郑连斌, 宇克莉, 宋雪, 田金源, 董文静. 大凉山彝族成人皮褶厚度特征[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 107 -112 .
[6] 林永生, 裴建国, 邹胜章, 杜毓超, 卢丽. 清江下游红层岩溶及其水化学特征[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 113 -120 .
[7] 张茹, 张蓓, 任鸿瑞. 山西轩岗矿区耕地流失时空特征及其影响因子研究[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 121 -132 .
[8] 李贤江, 石淑芹, 蔡为民, 曹玉青. 基于CA-Markov模型的天津滨海新区土地利用变化模拟[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 133 -143 .
[9] 王梦飞, 黄松. 广西西江经济带的城市旅游经济空间关联研究[J]. 广西师范大学学报（自然科学版）, 2018, 36(3): 144 -150 .
版权所有 © 广西师范大学学报（自然科学版）编辑部
地址：广西桂林市三里店育才路15号　邮编：541004
电话：0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发