广西师范大学学报（自然科学版） ›› 2019, Vol. 37 ›› Issue (4): 103-110.doi: 10.16088/j.issn.1001-6600.2019.04.013

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白芷水提液镇痛作用及其机理研究

郭珺, 陈丹, 朱婵, 唐宗湘*   

  1. 南京中医药大学医学与生命科学学院,江苏南京210023
  • 收稿日期:2019-02-27 出版日期:2019-10-25 发布日期:2019-11-28
  • 通讯作者: 唐宗湘(1964—),男,湖南会同人,南京中医药大学教授,博导。E-mail:zongxiangtang1@163.com
  • 基金资助:
    国家自然科学基金面上项目(31471007,31771163)

Analgesic Effect and Analgesic Mechanism of Angelica dahurica Extracts

GUO Jun, CHEN Dan, ZHU Chan, TANG Zongxiang*   

  1. School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing Jiangsu 210023, China
  • Received:2019-02-27 Online:2019-10-25 Published:2019-11-28

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摘要： 采用浸尾实验、热辐射实验、辣椒素和福尔马林脚掌致痛实验以及Von-Frey动物行为学实验,探究白芷水提液(ADE)的镇痛作用,并通过原代细胞培养和钙成像技术探讨白芷水提液的镇痛作用机理。结果发现,白芷水提液可有效抑制热浴浸尾和热辐射引起的伤害性热痛以及由辣椒素或福尔马林诱导的疼痛。然而,在Trpv1基因敲除小鼠中,白芷水提液不能缓解因热刺激或辣椒素引起的小鼠疼痛行为。此外,在小鼠背根神经节神经元中,白芷水提液能显著抑制由辣椒素刺激引起的钙离子内流。可见白芷水提液具有显著的镇痛作用,其镇痛作用与抑制TRPV1通道功能紧密相关。

关键词: 白芷, 镇痛, TRPV1 , 热痛, 急性痛

Abstract: The analgesic effect of ADE was evaluated by a series of pain models, in which the pain behaviours were induced by tail immersion, thermal radiation, capsaicin and formalin tests, and Von-Frey mechanical stimulation, respectively. The primary cell culture and calcium imaging technique were then employed to investigate the functional mechanism of ADE in neurons from dorsal root ganglion (DRG). The results showed that the ADE substantially attenuated the acute pain induced by thermal, capsaicin, and formalin in mice. However, ADE did not suppress the thermal-or capsaicin-induced acute pain in Trpv1-/- mice. Moreover, ADE significantly inhibited the intracellular influx of Ca2+ induced by capsaicin in the TRPV1-positive DRG neurons. So these findings suggested that ADE exhibits an analgesic effect in pain models by reducing the activity of TRPV1.

Key words: Angelica dahurica, analgesic effect, TRPV1, thermal pain, acute pain

中图分类号: 

  • R282.71
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