广西师范大学学报（自然科学版） ›› 2013, Vol. 31 ›› Issue (4): 121-127.

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黄鳝性逆转相关基因F4的克隆和表达分析

蒋骄云1,2, 冯龙2, 曲宪成2, 田文斐2   

  1. 1.广西师范大学生命科学学院,广西桂林541004;
    2.上海海洋大学水产与生命学院,上海201306
  • 收稿日期:2013-06-25 出版日期:2013-12-20 发布日期:2018-11-26
  • 通讯作者: 曲宪成(1965—),男,吉林榆林人,上海海洋大学副教授。E-mail:xcqu@shou.edu.cn
  • 基金资助:
    上海市重点学科建设基金资助项目(S30701);农业部科技教育司基金资助项目(201003076);上海高校知识服务平台上海海洋大学水产动物遗传育种中心基金资助项目(ZF1206)

Clone and Expression of a Novel Sex-related Gene (F4) in Gonads During Sex Reversal in the Rice Field Eel (Monopterus albus)

JIANG Jiao-yun1,2, FENG Long2, QU Xian-cheng2, TIAN Wen-fei2   

  1. 1.College of Life Sciences,Guangxi Normal University,Guilin Guangxi 541004,China;
    2.College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China
  • Received:2013-06-25 Online:2013-12-20 Published:2018-11-26

摘要： 本文运用cDNA末端快速扩增(Rapid-amplification of cDNA ends,RACE)技术、半定量RT-PCR技术和原位杂交技术,克隆黄鳝Monopterus albus性腺差异表达基因(F4)的cDNA全长序列,并分析该基因于各期性腺组织的表达情况。结果显示:F4基因cDNA全长2 466 bp,含有142 bp的5'-UTR、596 bp的3'-UTR、1 728 bp的开放阅读框(ORF),共编码575个氨基酸;在线Signal P分析表明,F4亚基肽链不存在信号肽,为非经典分泌蛋白;同源性分析结果显示该基因无同源性序列,视为新报道的基因;F4在卵巢发育早期不表达,从排卵的Ⅳ期卵巢开始表达,随着卵巢的败育和精巢的发育,表达量明显升高。性逆转过程中差异表达基因的克隆和鉴定,为进一步探究黄鳝性别调控机制奠定了基础。

关键词: 黄鳝, 性腺, 差异表达基因, RACE, RT-PCR, 原位杂交

Abstract: Using rapid-amplification of cDNA ends and semi-quantitative RT-PCR technique,the full length of the differentially expressed gene (F4) was obtained and the expression in the rice field eel (Monopterus albus) was conducted at different gonad developmental stage.The results showed that the full length of F4 was 2 466 base pair (bp) that comprises of a 5'-UTR of 142 bp,a 3'-UTR of 596 bp and an open reading frame(ORF) of 1 728 bp which encoded a 575 amino acid with no signal peptide.Homology analysis suggested that it was a newly-reported gene.F4 did not transcription have any at the early stage of ovary and began to express in ovary at Ⅳ stage,with the ovary abortive and testis development the transcription level was significantly increased.These results may provide molecular base for further study on sex regulation mechanism of the rice field eel.

Key words: Monopterus albus, gonad, differentially expressed gene, RACE, RT-PCR, in situ hybridization

中图分类号: 

  • Q786
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