广西师范大学学报（自然科学版） ›› 2017, Vol. 35 ›› Issue (4): 91-97.doi: 10.16088/j.issn.1001-6600.2017.04.013

• • 上一篇    下一篇

多肽探针结合纳米银催化反应-吸收测定HCG

李重宁1,2,潘宏程1*,刘庆业2,梁爱惠2,蒋治良2*   

  1. 1.桂林理工大学化学与生物工程学院,广西桂林541006;
    2. 广西师范大学环境与资源学院,广西桂林541004
  • 出版日期:2017-07-25 发布日期:2018-07-25
  • 通讯作者: 蒋治良(1965—),男,广西全州人,广西师范大学教授,博导。E-mail: zljiang@mailbox.gxnu.edu.cn
    潘宏程(1975—),男,广西平乐人,桂林理工大学教授,博导。E-mail: hcpan@163.com
  • 基金资助:
    国家自然科学基金(21667006,21567005, 21465006,21477025, 21367005);广西自然科学基金(2013GXNSFFA019003)

Peptide Probe Combined Nanosilver Catalytic Reaction-SurfacePlasmon Resonance Spectrophotometric Detectionfor Trace Human Chorionic Gonadotropin

LI Chongning1,2, PAN Hongcheng1*, LIU Qingye2, LIANG Aihui2, JIANG Zhiliang2*   

  1. 1. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin Guangxi 541006, China;
    2. College of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541004, China
  • Online:2017-07-25 Published:2018-07-25

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摘要： 在pH7.4的Na2HPO4-NaH2PO4缓冲溶液及人绒毛膜促性腺激素多肽探针(HCGP)存在下,银纳米粒子(AgNPs)发生聚集;当加入人绒毛膜促性腺激素(HCG)后,形成稳定的HCGP-HCG复合物,AgNPs能够稳定地分散在溶液中。该多肽反应液中分散的AgNPs对H2O2还原HAuCl4生成金纳米粒子的反应具有较强的催化作用,其产物金纳米微粒在550 nm处有一较强的表面等离子体共振(SPR)吸收峰。随着HCG浓度增大,反应液中分散的AgNPs增加,催化作用增强,550 nm处的吸光度增大。HCG浓度在0.5～15 μg/L与吸光度增大值ΔA550 nm成线性,检出限为0.2 μg/L。据此,建立了简便、价廉、灵敏检测HCG的多肽探针纳米银催化光度新方法。

关键词: 多肽探针, 人绒毛膜促性腺激素, AgNPs, 纳米催化, SPR光度法

Abstract: In pH 7.4 Na2HPO4-NaH2PO4 buffer solution with the presence of human chorionic gonadotropin peptide (HCGP) probe, the AgNPs can be aggregated to bigger AgNPs clusters. However, AgNPs can be well dispersed into this solution when the human chorionic gonadotropin (HCG) is added to form stable HCGP-HCG composite. The well-dispersed AgNPs exhibits catalysis on the reaction of H2O2 reducting HAuCl4 into AuNPs which appeares a strong surface plasmon resonance (SPR) peak at 550 nm. With the increase of HCG concentration, the SPR peak increases linearly at 550 nm. The increased SPR absorption (ΔA550 nm) is linear to HCG in the range of 0.25～50 μg/L, with a regression equation of ΔA550 nm=0.012 4C+0.02,a correlation coefficient of 0.987 8, and a detection limit of 0.2 μg/L. Thus, a simple, low-cost and sensitive nanosilver-catalytic SPR spectrometry was developed for the determination of HCG.

Key words: peptide probe, human chorionic gonadotropin, AgNPs, nanocatalysis, SPR absorption

中图分类号: 

  • O657.3
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