广西师范大学学报（自然科学版） ›› 2016, Vol. 34 ›› Issue (4): 143-150.doi: 10.16088/j.issn.1001-6600.2016.04.022

• 广西高校优秀中青年骨干教师培养工程论坛 • 上一篇    下一篇

2014—2015年武汉地区儿童乙型流感病毒 检测分析

蓝文华1,向赟2,艾洪武2,宋德贵1,吴建国3,熊鹰3,刘艳3,刘映乐3   

  1. 1.广西师范大学生命科学学院,广西桂林541006;
    2.武汉市儿童医院,湖北武汉430075;
    3.武汉大学生命科学学院,湖北武汉430075
  • 收稿日期:2016-04-09 出版日期:2016-07-18 发布日期:2018-07-18
  • 通讯作者: 宋德贵(1954—),男,广西博白人,广西师范大学教授。E-mail:sondegui@163.com
  • 基金资助:
    广西科学研究与技术开发计划项目基金资助(桂科重 14121003-3-1);国家科技重大专项课题基金资助(2012ZX10004207)

Distribution and Analysis of Children’s Influenza B virus in Wuhan during 2014-2015

LAN Wenhua1, XIANG Yun2, AI Hongwu2, SONG Degui1, WU Jianguo3, XIONG Ying3, LIU Yan3, LIU Yingle3   

  1. 1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Wuhan Children’s Hospital, Wuhan Hubei 430075, China;
    3. College of Life Science, Wuhan University, Wuhan Hubei 430075, China
  • Received:2016-04-09 Online:2016-07-18 Published:2018-07-18

PDF (PC)

118

摘要： 采用微量细胞板培养法分离流感病毒,RT-PCR、巢式PCR鉴定INFB及其HA1基因,运用Clustax、Mega 6.0、Megalign软件分析HA1核苷酸序列及氨基酸序列。结果:2014年4月—2015年3月共收集到发热呼吸道样本1 002株,分离到47株INFB阳性,分离出INFB阳性高峰出现在2014年8月和2015年1月,13~24个月儿童INFB阳性率最高, 0~6个月儿童阳性率最低。INFB患儿与患儿的性别及科室来源无显著关系,87.23% INFB患儿出现了发热、咳嗽症状。HA1片段分析结果表明,检测到的INFB属于Victoria系,与参考株B/Brisbane/60/2008核苷酸同源性在86.1%~88.0%,与B/HubeiJingzhouSongzi/1302/2010核苷酸同源性在86.1%~89.5%。3个氨基酸位点发生转换。结论:武汉地区儿童感染的INFB属于Victoria系,与北半球国际疫苗株B/Brisbane/60/2008、B/Texas/AF2741/2010以及湖北省INFB代表毒株B/Hubei JingzhouSongzi/1302/2010具有较高的亲缘关系,未发现新变种。

关键词: INFB, RT-PCR, 巢式PCR, HA1基因

Abstract: Using micro plate method to isolate the influenza virus, Influenza B virus and its HA1 gene were identified by RT-PCR and Nested-PCR,The nucleotide sequence of HA1 gene were analyzed by Clustax, Mega6.0,Megalign. Results:47 influenza B positive samples were isolated from 1 002 fever samples collected from April, 2014 - to March, 2015. The highest isolation rate was found in August 2014, and January, 2015. The highest influenza B positive was found in 13~24 months old children,and the lowest positive rate was found in 0~6 months old children. There is no significant difference among children patients with influenza B virus infection in either-gender or departments source:87.23% of children of the Influenza B virus infection show the symptoms of fever and cough. HA1 fragment analysis indicates the isolated influenza B virus belongs to the Victoria strain, and shows a 86.1%~88.0% homology to the reference strain, B/Brisbane/60/2008 in nucleotide sequence. Three amino acids of the isolated strain were different from the reference strain. Conclusion:Influenza B virus isolated in Wuhan belongs to the Victoria strain, which has high homology with the international vaccine strain B/Brisbane/60/2008 and B/Texas/AF2741/2010 in the northern hemisphere and INFB representative strains in Hubei province. No new strain was found.

Key words: INFB, RT-PCR, nested PCR, HA1 gene

中图分类号: 

  • R373.1
[1] 刘海燕, 林新勤, 唐昭, 等. 流感病毒研究概况[J]. 右江民族医学院学报, 2006, 27(6):909-911.
[2] 林丽华,谢剑锋,郑奎城.乙型流感病毒凝素和神经氨酸酶基因变异的研究进展[J].海峡预防医学杂志,2016(4):13-16.
[3] 杨帆, 廖国阳. 乙型流感病毒检测方法的研究进展[J]. 国际检验医学杂志, 2013, 10:29.
[4] COIRAS M T, P REZ-BRE A P, GARC A M L, et al. Simultaneous detection of influenza A, B, and C viruses, respiratory syncytial virus, and adenoviruses in clinical samples by multiplex reverse transcription nested-PCR assay[J]. J Med Virol, 2003, 69:132-144.
[5] 黄文林.分子病毒学[M].2版.北京:人民卫生出版社,2006:434-439.
[6] 刘传楠, 朱洪浩. 1994—1995 年度武汉市流感监测概况[J]. 湖北预防医学杂志, 1996, 7(2):1-2.
[7] 谢正德, 肖艳, 刘春艳, 等. 儿童急性下呼吸道感染病毒病原学 2007—2010 年监测[J]. 中华儿科杂志, 2011, 49(10):745-749.
[8] CHARLAND K M L, BUCKERIDGE D L, STURTEVANT J L, et al. Effect of environmental factors on the spatio-temporal patterns of influenza spread[J]. Epidemiology and Infection, 2009, 137(10):1377-1387.
[9] CHEN G W, SHIH S R, HSIAO M R, et al. Multiple genotypes of influenza B viruses cocirculated in Taiwan in 2004 and 2005[J]. Journal of Clinical Microbiology, 2007, 45(5):1515-1522.
[10] 高燕,方立群,张勇,等.中国大陆季节性流感活动的时空分布特征[J].中华流行病学杂志,2009,30(11):1097-1110.
[11] 何翠, 张磊, 李科, 等. 北京地区 2009—2010 年乙型流感病毒的流行特征及 HA1 基因片段特性分析[J]. 中国预防医学杂志, 2010(10):995-999.
[12] 于娟, 饶华祥, 李红, 等. 青海省 2011—2012 年乙型流感病毒 HA1 基因特性研究[J]. 中国人兽共患病学报, 2014, 30(1):32-35.
[13] 方斌,刘琳琳,李翔,等.2010—2015年湖北省乙型流感病毒流行和进化分析[J].疾病监测,2016,31(7):554-560.
[14] 肖芳,李健雄,周珺,等.2013—2014年江西省乙型流感病毒HA1基因特性分析[J].中国卫生检验杂志,2015(17):2943-2946.
[1] 蒋骄云, 冯龙, 曲宪成, 田文斐. 黄鳝性逆转相关基因F4的克隆和表达分析[J]. 广西师范大学学报（自然科学版）, 2013, 31(4): 121-127.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 广西师范大学学报（自然科学版）编辑部
地址：广西桂林市三里店育才路15号　邮编：541004
电话：0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发