锰在短毛蓼不同器官中的亚细胞分布及化学形态

广西师范大学学报（自然科学版） ›› 2010, Vol. 28 ›› Issue (1): 58-62.

锰在短毛蓼不同器官中的亚细胞分布及化学形态

邓华^1,2,3, 李明顺^1,3, 陈英旭², 于方明^1,3, 陈春强^1,3, 周振明^1,3

1.广西师范大学环境与资源学院,广西桂林 541004;
2.浙江大学环境与资源学院,浙江杭州 310029;
3.广西环境工程与保护评价重点实验室,广西桂林 541004

收稿日期:2009-12-18 出版日期:2010-03-20 发布日期:2023-02-07
通讯作者: 李明顺(1965—),男,湖北嘉鱼人,广西师范大学教授,博士。E-mail:msli@mailbox.gxnu.edu.cn
基金资助:
国家自然科学基金资助项目(30560032);广西重点实验室基金资助项目(0702k23);广西师范大学青年基金资助项目

Subcellular Distribution and Chemical Fractions of Manganese in Hyperaccumulator Polygonum pubescens Blume

DENG Hua^1,2,3, LI Ming-shun^1,3, CHEN Ying-xu², YU Fang-ming^1,3, CHEN Chun-qiang^1,3, ZHOU Zhen-ming^1,3

1. Department of Environmental Engineering,Guangxi Normal University,Guilin Guangxi 541004,China;
2. Department of Environmental Engineering,Zhejiang University,Hangzhou Zhejiang 310029,China;
3. Guangxi Key Laboratory of Enbironmental Engineering,Protection and Assessment,Guilin Guangxi 541004,China

Received:2009-12-18 Online:2010-03-20 Published:2023-02-07

摘要/Abstract

摘要： 研究锰在超富集植物短毛蓼Polygonum pubescens Blume体内的亚细胞分布及化学形态。采用差速离心法及化学试剂逐步提取法。结果表明,在亚细胞水平分布上,短毛蓼根、茎、叶中90％以上的锰分布在细胞壁和可溶性部分(包括细胞质和液泡);在锰赋存形态上,茎部以水提取态和氯化钠提取态为主,根部和叶部以水提取态、氯化钠提取态和盐酸提取态为主;随着锰处理浓度增加,根中锰由水提取态向盐酸提取态和氯化钠提取态转化,叶中水提取态和盐酸提取态Mn占总Mn的比例逐渐上升。细胞壁和液泡是Mn在短毛蓼细胞内的主要贮存点位,Mn在短毛蓼中的化学形态以水提取态、盐酸提取态和氯化钠提取态为主。

关键词: 锰, 短毛蓼, 亚细胞分布, 化学形态

Abstract: The experiments were carried out to find out subcellular distribution characteristics and chemical forms of Mn in the leaves,stems and roots of Polygonum pubescens Blume,which is a newly found manganese hyperaccumulator.Differential centrifugation and sequential chemical extraction were used.More than 90% of total Mn was bound to cell wall and soluble fraction in theplant.The results from extraction experiments by using different extractants showed that water-extractable Mn and NaCl-extractable Mn predominated in stems,while water-extractable Mn,NaCl-extractable Mn and HCl-extractable Mn predominated in roots and leaves.With the Mn supply increasing,the percentage of Water-extractable Mn decreased while the percentage of HCl-extractable and NaCl-extractable Mn increased in the roots,and Water-extractable and HCl-extractable Mn increased in the leaves.Most of the Mn,which was accumulated by P.pubescens Blume,wasstored in the cell wall and the soluble fractions (including cytoplasm and karyon).For the chemical forms,Water,HCl and NaCl-extractable Mn predominated in the plant.

Key words: Manganese, Polygonum pubescens Blume, subcellulardistribution, chemical forms

中图分类号:

X171.5

邓华, 李明顺, 陈英旭, 于方明, 陈春强, 周振明. 锰在短毛蓼不同器官中的亚细胞分布及化学形态[J]. 广西师范大学学报（自然科学版）, 2010, 28(1): 58-62.

DENG Hua, LI Ming-shun, CHEN Ying-xu, YU Fang-ming, CHEN Chun-qiang, ZHOU Zhen-ming. Subcellular Distribution and Chemical Fractions of Manganese in Hyperaccumulator Polygonum pubescens Blume[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(1): 58-62.

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