哺乳藏猪肠道CAT1、EAAC1和Pept1 mRNA的发育性表达分析

广西师范大学学报（自然科学版） ›› 2010, Vol. 28 ›› Issue (1): 63-67.

哺乳藏猪肠道CAT1、EAAC1和Pept1 mRNA的发育性表达分析

陈敦学¹, 石常友¹, 宾石玉¹, 褚武英², 唐万林³, 林谦¹

1.广西师范大学生命科学学院,广西桂林 541004;
2.长沙大学生物工程与环境科学系,湖南长沙 410003;
3.桂林市水产畜牧兽医局,广西桂林 541004

收稿日期:2009-11-17 出版日期:2010-03-20 发布日期:2023-02-07
通讯作者: 宾石玉(1963—),男,广西全州人,广西师范大学教授,博士。E-mail:binsy68@yahoo.com.cn
基金资助:
国家自然科学基金资助项目(30640015)

CAT1,EAAC1 and Pept1 mRNA in Intestinal for Detection Developmental Expression in Tibetan

CHEN Dun-xue¹, SHI Chang-you¹, BIN Shi-yu¹, CHU Wu-ying², TANG Wang-lin³, LIN Qian¹

1. College of Life Science,Guangxi Normal University,Guilin Guangxi 514004,China;
2. Department of Biotechnology and Environmental Science,Changsha University,Changsha Hunan 410003,China;
3. Aquatic Animal Husbandry and Veterinary Bureau of Guilin,Guilin Guangxi541004,China

Received:2009-11-17 Online:2010-03-20 Published:2023-02-07

摘要/Abstract

摘要： 选取体重接近且健康的哺乳藏猪30头,其中1、7、14、21和28日龄各6头,运用实时定量PCR对Pept1、EAAC1、CAT1 mRNA表达的发育性变化进行研究。结果表明,在十二指肠中藏猪CAT1、Pept1 mRNA表达的发育性变化相似,14日龄时表达丰度最高;EAAC1、CAT1 mRNA在空肠中表达的发育性变化相似,CAT1在21日龄达到最高点,但与其他日龄相比差异不显著;回肠中Pept1与EAAC1 mRNA表达丰度具有相似的发育性变化,7日龄回肠Pept1 mRNA表达量最高,显著高于其他各发育阶段(P＜0.05)。

关键词: 哺乳藏猪, 肠道, 转运载体, 发育性表达, 实时定量PCR

Abstract: 30 healthy Tibetan piglets with similar body weight(6of suckling Tibetan piglets of 1 d,7 d,14 d,21 d and 28 d,respectically) are chosen for detecting developmental expression of Pept1,EAAC1 and CAT1 mRNA through real time PCR.Results show that there are greater significance for ontogenesises of Pept1,EAAC1 and CAT1 mRNA expression along the horizontal axis of the small intestine of suckling Tibetal piglets.Ontogenesises of Pept1 and CAT1 mRNA expressionin duodenum are similar,while 14-day-old expression of the highest abundance,compared with other developmental stages,was significant(P＜0.01);The expression of EAAC1,CAT1 mRNA developmental changes are similar in Jejunum,the expression of CAT1 in the 21-day-old Tibetan piglets reach the highestpoint,but compared with others no significant difference;Ontogenesises of Pept1 and CAT1 mRNAexpression in ileum are similar,Pept1 and CAT1 mRNA expression abudance both are highest at 7-day-old.

Key words: suckling Tibetan piglet, intestinal, developmental expression, real-time PCR, oligopeptide transporter

中图分类号:

S852.23

陈敦学, 石常友, 宾石玉, 褚武英, 唐万林, 林谦. 哺乳藏猪肠道CAT1、EAAC1和Pept1 mRNA的发育性表达分析[J]. 广西师范大学学报（自然科学版）, 2010, 28(1): 63-67.

CHEN Dun-xue, SHI Chang-you, BIN Shi-yu, CHU Wu-ying, TANG Wang-lin, LIN Qian. CAT1,EAAC1 and Pept1 mRNA in Intestinal for Detection Developmental Expression in Tibetan[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(1): 63-67.

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