rhFGF-8a制备及其对鸡胚绒毛尿囊膜血管生成的影响

广西师范大学学报（自然科学版） ›› 2010, Vol. 28 ›› Issue (1): 68-71.

rhFGF-8a制备及其对鸡胚绒毛尿囊膜血管生成的影响

周飞燕^1,2, 卢柳燕³, 韦剑², 丘力功², 黄明^1,2

1.广西师范大学生命科学学院,广西桂林 541004;
2.广州拜迪生物医药研究开发中心,广东广州 511495;
3.深圳生科源技术有限公司,广东深圳 518172

收稿日期:2009-11-02 出版日期:2010-03-20 发布日期:2023-02-07
通讯作者: 黄明(1962—),男,湖南桂阳人,广西师范大学外聘教授,广州拜迪生物医药有限公司总经理,博士,硕导。E-mail:btmhuang@163.com
基金资助:
广东省自然科学基金资助项目(04006313)

Preparation of Recombinant Human Fibroblast Growth Factor 8a andits Effect on Chick Embryo Chorioallantoic Membrane Angiogenesis

ZHOU Fei-yan^1,2, LU Liu-yan³, WEI Jian², QIU Li-gong², HUANG Ming^1,2

1. College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China;
2. Guangzhou Baidi Biotechnology CO.LTD,Guangzhou Guangdong 511495,China;
3. MABSKY Biotech Inc,Shenzhen Guangdong 518172,China

Received:2009-11-02 Online:2010-03-20 Published:2023-02-07

摘要/Abstract

摘要： 为研究rhFGF-8a对鸡胚绒毛尿囊膜新生血管生成的影响,通过基因工程方法获得了rhFGF-8a包涵体蛋白的大量表达,经复性、纯化后获得纯度高达99%以上的rhFGF-8a蛋白。通过细胞增殖法测定rhFGF-8a的生物学活性,运用鸡胚绒毛尿囊膜实验观察其对新生血管生成的影响,结果表明:纯化后的rhFGF-8a能够刺激小鼠成纤维细胞NIH 3T3的生长;rhFGF-8a诱发鸡胚绒毛尿囊膜新生血管生成能力很弱。

关键词: rhFGF-8a, 鸡胚绒毛尿囊膜, 血管生成

Abstract: To investigate the effect of rhFGF-8a to chick embryo chorioallantoic membrane angiogenesis.RhFGF-8a protein is over-expressed in E.coli through genetic engineering technology and insoluble components is about 50% of the total protein.RhFGF-8a protein is refoled andpurified and its purity can reach 99% identified by SDS-PAGE.The biologicalactivity of rhFGF-8a is determined by MTT and its effect on angiogenesis is identified by chick embryo chorioallantoic membrane(CAM) experiments.The results showthat the purified rhFGF-8a protein can stimulate the growth of NIH 3T3 cells and rhFGF-8aprotein has weak capacity on angiogenesis in CAM.

Key words: rhFGF-8a, chick embryo chorioallantoic membrane, angiogenesis

中图分类号:

R979.1

周飞燕, 卢柳燕, 韦剑, 丘力功, 黄明. rhFGF-8a制备及其对鸡胚绒毛尿囊膜血管生成的影响[J]. 广西师范大学学报（自然科学版）, 2010, 28(1): 68-71.

ZHOU Fei-yan, LU Liu-yan, WEI Jian, QIU Li-gong, HUANG Ming. Preparation of Recombinant Human Fibroblast Growth Factor 8a andits Effect on Chick Embryo Chorioallantoic Membrane Angiogenesis[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(1): 68-71.

参考文献

[1] MARSH S K,BANSAL G S,ZAMMITC,et al.Increased expression of fibroblast growth factor 8 in human breast cancer[J].Oncogene,1999,18(4):1053-1060.
[2] VALTA M P,HENTUNEN T,QU Qiang,et al.Regulation of osteoblast differentiation:a novel function for fibroblast growth factor 8[J].Endocrinology,2006,147(5):2171-2182.
[3] PAYSON R A,WU J,LIU Y,et al.The human FGF-8 gene localizes on chromosome 10q24 and is subjected to induction by androgen in breast cancer cells[J].Oncogene,1996,13(1):47-53.
[4] MACARTHUR C A,LAWSHE A,SHANKAR D B,et al.FGF-8 Isoforms differ in NIH3t3 cell transforming potential[J].Cell Growth & Differentiation,1995,6(7):817-825.
[5] ZAMMIT C,COOPE R,GOMM J J,et al.Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer[J].British Journal of Cancer,2002,86(7):1097-1103.
[6] MATTILA M M,TARKKONEN K M,SEPPANEN J A,et al.Androgen and fibroblast growth factor 8 (FGF8) downregulation of thrombospondin 1 (TSP1) in mouse breast cancer cells[J].Molecular and Celluar Endocrinology,2006,253(1/2):36-43.
[7] 国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2005:106.
[8] 胡定慧,马润娣,于立坚.土贝母苷甲抑制血管生成[J].中国药理学通报,2003,19(6):713-715.
[9] 张维东,崔亚洲,姚成芳,等.蝎毒多肽提取物抗肿瘤血管生成作用的实验研究[J].中国药理学通报,2005,21(6):708.
[10] HERNáNDEZ-MARTíNEZ R,CASTRO-OBREGóN S,COVARRUBIAS L.Progressive interdigital cell death:regulation by the antagonistic interaction between fibroblast growth factor 8 and retinoic acid[J].Development,2009,136(21):3669-3678.
[11] MATTILA M M,HA¨RKO¨NEN P L.Role of fibroblast growth factor 8 in growth and progression of hormonal cancer[J].Cytokine & Growth Factor Reviews,2007,18(3):257-266.

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