拟南芥DnaJ蛋白的过量表达对细菌耐盐性的影响

广西师范大学学报（自然科学版） ›› 2010, Vol. 28 ›› Issue (1): 54-57.

拟南芥DnaJ蛋白的过量表达对细菌耐盐性的影响

赵志常, 张建军, 张皖蓉, 刘震, 李旭锋, 杨毅

四川大学生命科学学院生物资源与生态环境教育部重点实验室,四川成都 610064

收稿日期:2009-11-01 出版日期:2010-03-20 发布日期:2023-02-07
通讯作者: 杨毅(1963—),男,四川成都人,四川大学教授,博导。E-mail:yangyi528@vip.sina.com
基金资助:
国家教育部重点项目(105140)

Over-expression of Arabidopsis DnaJ (Hsp40) Contributes to NaCl-stress Tolerance of Escherichia coli

ZHAO Zhi-chang, ZHANG Jian-jun, ZHANG Wan-rong, LIU Zhen, LI Xu-feng, YANG Yi

Key Laboratory of Bio-Resources and Eco-Environment of EOM,College of Life Sciences,Sichuan University, Chengdu Sichuan 610064,China

Received:2009-11-01 Online:2010-03-20 Published:2023-02-07

摘要/Abstract

摘要： 热激蛋白是植物在各种环境胁迫下产生的一种蛋白,其表达与多种胁迫的抗性有一定关系。从野生型拟南芥cDNA中克隆获得了编码442个氨基酸序列的DnaJ基因,其与大肠杆菌DnaJ基因含有相同的J区和半胱氨酸富集区。将DnaJ基因构建到pET32a的原核表达载体,过量表达DnaJ基因的细菌在含有0.5 mol/L NaCl的培养基中仍然可以生长,初步推断DnaJ的表达与耐盐性有关。

关键词: 热激蛋白, DnaJ, 过量表达, 细菌耐盐性

Abstract: Heat shock proteins of plants were main protein in some evolution stress.Expression of some heat shock proteins had a certain relationship with plant tolerance.The nucleotide sequence of a cDNA cloned from Arabidopsis thaliana ecotype RLD was determined,and the corresponding amino sequence deduced.The open reading frame encodes a DnaJ protein of 442 residues with a molecular mass of 47 ku.The predicted sequence contains regions homologous to theJ-and cysteine-rich domains of Escherichia coli DnaJ.The cDNA fragment directionally cloned into the vector pET32α,over-expression of DnaJ in E.coli,the E.coli could grow on LB containing 0.5 mol/L NaCl.

Key words: heat shock protein, DnaJ, over-expression, NaCl tolerance of E.coli

中图分类号:

赵志常, 张建军, 张皖蓉, 刘震, 李旭锋, 杨毅. 拟南芥DnaJ蛋白的过量表达对细菌耐盐性的影响[J]. 广西师范大学学报（自然科学版）, 2010, 28(1): 54-57.

ZHAO Zhi-chang, ZHANG Jian-jun, ZHANG Wan-rong, LIU Zhen, LI Xu-feng, YANG Yi. Over-expression of Arabidopsis DnaJ (Hsp40) Contributes to NaCl-stress Tolerance of Escherichia coli[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(1): 54-57.

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