大石围天坑群生境土壤理化性质与球囊霉素的关系

doi:10.16088/j.issn.1001-6600.2025082602

广西师范大学学报（自然科学版） ›› 2026, Vol. 44 ›› Issue (4): 185-194.doi: 10.16088/j.issn.1001-6600.2025082602

大石围天坑群生境土壤理化性质与球囊霉素的关系

黄宏盛^1,2,3, 宾国梁^1,2,3, 卢世纪^1,2,3, 宁紫月^1,2,3, 杜晓月^1,2,3, 薛跃规^1,2,3*, 林凡⁴

1.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西桂林 541006;
2.广西漓江流域景观资源保育与可持续利用重点实验室(广西师范大学), 广西桂林 541006;
3.广西师范大学生命科学学院, 广西桂林 541006;
4.中国乐业-凤山世界地质公园, 广西百色 533299

收稿日期:2025-08-26 修回日期:2025-10-28 出版日期:2026-07-05 发布日期:2026-07-01
通讯作者: 薛跃规(1964—),男,湖南益阳人,广西师范大学教授,博士。E-mail: xueyuegui@126.com
基金资助:
国家自然科学基金(31960047);广西重点研发计划(桂科AB21220057);珍稀濒危动植物生态与环境保护教育部重点实验室研究基金(ERESEP2019Z04);广西漓江流域景观资源保育与可持续利用重点实验室研究基金(LRCSU21Z0101)

Relationship between soil physicochemical properties and glomalin in habitats of Dashiwei Tiankengs

Huang Hongsheng^1,2,3, Bin Guoliang^1,2,3, Lu Shiji^1,2,3, Ning Ziyue^1,2,3, Du Xiaoyue^1,2,3, Xue Yuegui^1,2,3*, Lin Fan⁴

1. Key Laboratory of Ecology and Environmental Protection of Rare and Endangered Animals and Plants (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
2. Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in the Lijiang River Basin (Guangxi Normal University), Guilin Guangxi 541006, China;
3. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
4. Leye-Fengshan World Geopark of China, Baise Guangxi 533299, China

Received:2025-08-26 Revised:2025-10-28 Online:2026-07-05 Published:2026-07-01

摘要/Abstract

摘要： 为揭示广西大石围天坑群异质生境(坑内、坑口、坑外)球囊霉素相关土壤蛋白(GRSP)的分布特征及其与土壤理化性质的关系,本研究选取天坑3类小生境土壤为研究对象,测定易提取球囊霉素相关土壤蛋白(EE-GRSP)和总球囊霉素相关土壤蛋白(T-GRSP)的含量,结合土壤理化指标,运用Spearman相关性分析及冗余分析等方法,系统分析 GRSP 的生境分异特征,并探究其与土壤理化因子的相关性。结果表明:在3类小生境中,EE-GRSP含量在坑外(1.55 mg/g)显著高于坑内(1.35 mg/g),坑口(1.52 mg/g)与二者相比无显著差异;T-GRSP含量为3.17~3.51 mg/g,各生境间无显著差异;EE-GRSP占有机碳的比例(EE-GRSP/SOC)为3.51%~4.72%,坑口的比例显著低于坑外及坑内,T-GRSP占有机碳的比例(T-GRSP/SOC)在各生境间无显著差异。土壤理化因子与GRSP的关联分析显示,坑内有机碳(SOC)、坑口全氮(TN)、全碳(TC)和坑外全碳是影响GRSP含量的核心土壤因子,且GRSP的积累随土壤碳氮储量的增加而增加,GRSP的SOC占比则因积累速率滞后于土壤碳氮而随土壤碳氮储量的增加而降低。此外,土壤水分、pH和全磷(TP)对GRSP的影响具有生境特异性。研究证实,大石围天坑GRSP的积累受生境异质性和土壤碳氮因子共同影响, 其碳氮固存功能在喀斯特天坑生态系统的土壤质量维持中具有关键作用。

Abstract: To reveal the distribution characteristics of glomalin-related soil protein (GRSP) in the heterogeneous habitats (inside, fringe, and outside) of the Guangxi Dashiwei Tiankengs and its relationship with soil physicochemical properties, soils from these three microhabitats of the tiankeng were selected as the research objects. The contents of easily extractable glomalin-related soil protein (EE-GRSP) and total glomalin-related soil protein (T-GRSP) were determined. Combined with soil physicochemical indicators, methods such as Spearman correlation analysis and redundancy analysis were employed to systematically analyze the habitat differentiation characteristics of GRSP and explore its correlation with soil physicochemical factors. The results showed that among the three microhabitats, EE-GRSP was significantly higher outside the tiankeng (1.55 mg/g) than inside (1.35 mg/g), while no significant habitat difference was observed at the Fringe (1.52 mg/g). The T-GRSP content ranged from 3.17 to 3.51 mg/g, with no significant differences among habitats. The proportion of EE-GRSP to organic carbon (EE-GRSP/SOC) ranged from 3.51% to 4.72%, with the Fringe being significantly lower than outside and inside. The proportion of T-GRSP to organic carbon (T-GRSP/SOC) showed no significant differences among habitats. Based on the relationship between soil physicochemical factors and GRSP, it was indicated that organic carbon (SOC) inside the tiankeng, total nitrogen (TN) and total carbon (TC) at the Fringe, and total carbon (TC) outside the tiankeng were the core soil factors affecting GRSP content. Moreover, the accumulation of GRSP increases with the increase in soil carbon and nitrogen storage, while the proportion of GRSP to SOC decreased with the increase in soil carbon and nitrogen storage due to its accumulation rate lagging behind that of soil carbon and nitrogen. Additionally, the effects of soil moisture, pH, and TP on GRSP were habitat-specific. The study confirmed that the accumulation of GRSP in the tiankeng was influenced by habitat heterogeneity and soil carbon-nitrogen factors, and its carbon and nitrogen sequestration function played a key role in maintaining soil quality in the karst tiankeng ecosystem.

Key words: karst tiankeng, glomalin-related soil protein, soil organic carbon, habitat heterogeneity, arbuscular mycorrhizal fungi

中图分类号: X144; S153

黄宏盛, 宾国梁, 卢世纪, 宁紫月, 杜晓月, 薛跃规, 林凡. 大石围天坑群生境土壤理化性质与球囊霉素的关系[J]. 广西师范大学学报（自然科学版）, 2026, 44(4): 185-194.

Huang Hongsheng, Bin Guoliang, Lu Shiji, Ning Ziyue, Du Xiaoyue, Xue Yuegui, Lin Fan. Relationship between soil physicochemical properties and glomalin in habitats of Dashiwei Tiankengs[J]. Journal of Guangxi Normal University(Natural Science Edition), 2026, 44(4): 185-194.

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大石围天坑群生境土壤理化性质与球囊霉素的关系

Relationship between soil physicochemical properties and glomalin in habitats of Dashiwei Tiankengs

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