2025年04月13日 星期日

广西师范大学学报（自然科学版） ›› 2025, Vol. 43 ›› Issue (1): 101-109.doi: 10.16088/j.issn.1001-6600.2024040309

• “生态保护与资源可持续利用”专辑 • 上一篇    下一篇

脐橙皮水溶性膳食纤维提取工艺优化及其体外益生活性评价

王月蓉1,2,3, 赵广河1,2,3,4*, 赵丰丽1,2,3, 覃云斌1,2,3, 陈静1,2,3, 张弘1,2,3   

  1. 1.广西师范大学 生命科学学院, 广西 桂林 541006;
    2.广西漓江流域景观资源保育与可持续利用重点实验室(广西师范大学),广西 桂林 541006;
    3.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学),广西 桂林 541006;
    4.广西师范大学 应用生物学研究所,广西 桂林 541004
  • 收稿日期:2024-04-03 修回日期:2024-05-17 出版日期:2025-01-05 发布日期:2025-02-07
  • 通讯作者: 赵广河(1977—),男,河南南阳人,广西师范大学副教授,博士。E-mail:377325019@qq.com
  • 基金资助:
    桂林市科学研究与技术开发计划项目(20210217-18);广西科技厅重点研发计划项目(2021AB27009)

Optimization of Extraction Process of Navel Orange Peel Soluble Dietary Fiber and Its in vitro Prebiotic Activity Evaluation

WANG Yuerong1,2,3, ZHAO Guanghe1,2,3,4*, ZHAO Fengli1,2,3, QIN Yunbin1,2,3, CHEN Jing1,2,3, ZHANG Hong1,2,3   

  1. 1. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), Guilin Guangxi 541006, China;
    3. Key Laboratory of Ecology of Rare and Endangered Species and Enviromental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    4. Institute of Applied Biology, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2024-04-03 Revised:2024-05-17 Online:2025-01-05 Published:2025-02-07

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摘要： 以脐橙皮作为原料,采取超声协同纤维素酶辅助提取脐橙皮水溶性膳食纤维(SDF),通过单因素及响应面试验对脐橙皮SDF的提取工艺进行优化,并评价其体外益生活性。结果表明,超声协同酶解辅助提取脐橙皮SDF的优化工艺条件是:液料比16 mL/g、超声功率 45 W/L、超声时间40 min、纤维素酶添加量10.0 μL/g,该条件下脐橙皮SDF 提取得率为11.88%。体外发酵实验表明,超声协同酶解显著改善了SDF的体外益生活性。因此,超声协同酶解可被认为是一种对脐橙皮膳食纤维进行改性的优良方法,可为其在功能食品中的应用提供参考。

关键词: 脐橙皮, 水溶性膳食纤维, 超声波, 纤维素酶, 益生活性

Abstract: The soluble dietary fiber (SDF) in navel orange peel was extracted by ultrasonic wave combined with cellulase-assisted extraction method. The extraction process of SDF was optimized by single factor test and response surface methodology and its in vitro prebiotic activity was evaluated, so as to provide technical reference for developing high quality navel orange peel SDF. The results showed that the optimal conditions to extract orange peel SDF of ultrasonic wave combined with cellulase-assisted extraction method were as follows: liquid-solid ratio 16 mL/g, ultrasonic power 45 W/L, ultrasonic time 40 min and enzyme addition 10.0 μL/g. Under these conditions,the extraction yield of SDF was 11.88%. In vitro fermentation experiments showed that the SDF from modified navel orange peel by ultrasonic wave combined with cellulose treatment exhibited excellent probiotic activity. Therefore, ultrasonic wave combined with cellulase hydrolysis can be considered as an excellent method to modify dietary fiber of navel orange peel, which can provide reference for its application in functional foods.

Key words: navel orange peel, water-soluble dietary fiber, ultrasonic wave, cellulase, probiotic activity

中图分类号:  TS209

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