广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (2): 175-189.doi: 10.16088/j.issn.1001-6600.2021111702

• 研究论文 • 上一篇    下一篇

基于高通量测序技术研究环境微生物群落结构特征

肖飞1,2, 丁旭升1, 樊培培1, 王维红1*   

  1. 1.新疆农业大学 水利与土木工程学院,新疆 乌鲁木齐 830052;
    2.塔里木大学 水利与建筑工程学院,新疆 阿拉尔 843300
  • 收稿日期:2021-11-17 修回日期:2022-01-12 出版日期:2023-03-25 发布日期:2023-04-25
  • 通讯作者: 王维红(1967—),女,新疆奇台人,新疆农业大学教授。E-mail:2209319288@qq.com
  • 基金资助:
    国家自然科学基金(51968071)

Structural Characteristics of Environmental Microbial Community Studied by High-throughput Sequencing Technology

XIAO Fei1,2, DING Xusheng1, FAN Peipei1, WANG Weihong1*   

  1. 1. College of Water Conservancy and Civil Engineering, Xinjiang Agricultural University, Urumqi Xinjiang 830052, China;
    2. College of Water Resources and Architectural Engineering, Tarim University, Aral Xinjiang 843300, China
  • Received:2021-11-17 Revised:2022-01-12 Online:2023-03-25 Published:2023-04-25

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摘要： 采用高通量测序技术对序批式反应器(R1、R2处理)中微生物群落结构的演变及多样性进行分析,同时对细菌群落与人工模拟番茄酱废水水质相关性进行分析,对微生物酶进行预测。结果表明:微生物菌群在门、纲和属水平上存在显著差异,其中门类水平上,Proteobacteria(变形菌门)和Bacteroidetes(拟杆菌门)为优势菌门;纲水平上,Betaproteobacteria(β-变形菌纲)、Alphaproteobacteria(α-变形杆菌纲)和Bacteroidia(拟杆菌纲)为优势菌纲;属水平上,Zoogloea(动胶菌属)和Flavobacterium(黄杆菌属)为优势菌属。在颗粒形成初期至稳定期,R1、R2中Proteobacteria、Bacteroidetes、Betaproteobacteria、Alphaproteobacteria、Bacteroidia、Zoogloea和Flavobacterium丰度变化分别为1.75%、-7.20%、35.49%、0.12%、-15.23%、-3.11%和-5.23%;1.78%、-2.00%、33.11%、-0.54%、-14.80%、-2.87%和6.13%,各处理的丰度变化较小,丰度差异见于Alphaproteobacteria、Bacteroidia和Flavobacterium。相关性分析表明,Sphingobacteriia和Negativicutes与NH3-N和PO3-4-P、Flavobacteria与COD呈显著负相关,Gammaproteobacteria、Clostridia与COD和NH3-N呈显著正相关,Betaproteobacteria与PO3-4-P呈显著正相关。Proteobacteria具有脱氮作用,Bacteroidetes与EPS的降解等有关,Acidobacteria对絮体污泥的初始凝聚具有积极作用。絮体期的污泥还存在Anaerolineae,Anaerolineae有利于絮体污泥的沉降。Delta-9 desaturase(硬脂酰辅酶A脱氢酶,ó-9脱氢酶)和transport system permease protein(系统通透酶蛋白)丰度较高,lactocepin(乳酸菌素)、linoleoyl-CoA desaturase(亚油酰辅酶A脱氢酶)和ribosomal protein S6 modification protein(核糖体蛋白S6修饰蛋白)变化最显著,但整个体系中的微生物酶保持有类似的功能。

关键词: 好氧颗粒污泥, 高通量技术, 微生物菌群, 番茄酱废水, 微生物酶

Abstract: High-throughput sequencing technology was used to analyze the evolution and diversity of microbial community structure in sequence batch reactors (R1, R2), the correlation between bacterial community and tomato sauce wastewater quality and the prediction of microbial enzymes. The results showed that there were significant differences in the level of phylum, class and genus. Proteobacteria and Bacteroidetes were the dominant phylum at the level of phylum. At class level, Betaproteobacteria, Alphaproteobacteria and Bacteroidia were the dominant bacteria. At the genus level, Zoogloea and Flavobacterium were the dominant genera. From the initial stage of particle formation to the stable stage, the abundances of Proteobacteria, Bacteroidetes, Betaproteobacteria, Alphaproteobacteria, Bacteroidia, Zoogloea and Flavobacterium in R1 and R2 were 1.75%, -7.20%, 35.49%, 0.12%, -15.23%, -3.11% and -5.23% respectively. The abundance of 1.78%, -2.00%, 33.11%, -0.54%, -14.80%, -2.87% and 6.13% showed little variation. But the differences in abundance was found in Alphaproteobacteria, Bacteroidia and Flavobacterium. Correlation analysis showed that Sphingobacteriia and Negativicutes had a significant negative correlation with NH3-N and PO3-4-P, Flavobacteria had a significant negative correlation with COD. Gammaproteobacteria and Clostridia were significantly positively correlated with COD and NH3-N, while Betaproteobacteria was significantly positively correlated with PO3-4-P. Proteobacteria has the effect of nitrogen removal, Bacteroidetes is related to the degradation of EPS, Acidobacteria has a positive effect on the initial coagulation of flocs sludge. Anaerolineae also exists in the sludge at the floc stage, and Anaerolineae is beneficial to the settlement of the floc sludge. Among them delta-9 desaturase (stearoyl-CoA dehydrogenase (ó-9 dehydrogenase) and transport system permease protein (system permease protein) are more abundant, and lactocepin (lactocerin), linoleoyl-CoA desaturase (sub oleoyl-CoA dehydrogenase) and ribosomal protein S6 modification protein (ribosomal protein S6 modification protein) changed most significantly, but microbial enzymes throughout the system maintained similar functions.

Key words: aerobic granular sludge, high-throughput technology, microbial flora, tomato paste wastewater, microbial enzyme

中图分类号: 

  • X703
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