Journal of Guangxi Normal University(Natural Science Edition) ›› 2026, Vol. 44 ›› Issue (4): 210-220.doi: 10.16088/j.issn.1001-6600.2025082101

• Agricultural Science • Previous Articles     Next Articles

Composition and distribution of dissolved organic matter in farmland soils of typical karst area

Wei Maoyu1, Ou Yuqun1, Chen Xi1, Liao Lingling1,2, Pan Yinhua1,2*   

  1. 1. College of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions (Guangxi Normal University), Guilin Guangxi 541006, China
  • Received:2025-08-21 Revised:2025-10-01 Online:2026-07-05 Published:2026-07-01

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Abstract: Farmland soils developed in the karst regions of distinctive aboveground-underground coupled structure, generally exhibit serious soil erosion and nutrient deficiency, which constrains sustainable agricultural development of the regions. Soil organic matter, particularly the soluble low-molecular-weight fraction therein, is a key driver of soil fertility conservation. Thus, understanding of their composition and distribution is of great significance to improve soil nutrient availability and crop productivity. In this study, organic carbon abundance, chemical structure of dissolved organic matter (DOM), and species and contents of low-molecular-weight organic acids (LMWOAs) in typical farmland soils of the Guilin karst area, Guangxi, were investigated to examine their relationships with soil fertility. The results showed that the compositional structure of DOM were similar among the soils, but significant differences were observed in their contents. The composition of DOM is dominated by aliphatic compounds with a relatively low abundance of aromatic compounds while that of LMWOAs was comprised mainly of oxalic and formic acids. These components were enriched in the high-fertility soils but depleted in the low-fertility ones. Significant positive correlations were observed between SOM content and the contents of aliphatic compounds, aromatic compounds, and LMWOAs, suggesting that aliphatic and aromatic compounds served as effective components for enhancing soil fertility and soil structural stability while LMWOAs for facilitating soil nutrient cycling and dynamic balance. This has important implications for optimizing soil fertility management and guiding fertilization practices in karst farmlands.

Key words: dissolved organic matter, low-molecular-weight organic acids, soil fertility, karst area, farmland soil

CLC Number:  S158.2; P599
[1] 陈同斌, 陈志军. 土壤中溶解性有机质及其对污染物吸附和解吸行为的影响[J]. 植物营养与肥料学报, 1998, 4(3): 201-210.
[2] Thieme L, Graeber D, Hofmann D, et al. Dissolved organic matter characteristics of deciduous and coniferous forests with variable management: different at the source, aligned in the soil[J]. Biogeosciences, 2019, 16(7): 1411-1432. DOI: 10.5194/bg-16-1411-2019.
[3] Chen Z L, Jin X Y, Wang Q, et al. Confirmation and determination of carboxylic acids in root exudates using LC-ESI-MS[J]. Journal of Separation Science, 2007, 30(15): 2440-2446. DOI: 10.1002/jssc.200700234.
[4] Doelsch E, Masion A, Cazevieille P, et al. Spectroscopic characterization of organic matter of a soil and vinasse mixture during aerobic or anaerobic incubation[J]. Waste Management, 2009, 29(6): 1929-1935. DOI: 10.1016/j.wasman.2008.12.009.
[5] Moradi N, Sadaghiani M R, Sepehr E, et al. Effects of low-molecular-weight organic acids on phosphorus sorption characteristics in some calcareous soils[J]. Turkish Journal of Agriculture and Forestry, 2012: 36(4): 459-468. DOI: 10.3906/tar-1106-38.
[6] 莫淑勋. 土壤中有机酸的产生、转化及对土壤肥力的某些影响[J]. 土壤学进展, 1986, 14(4): 1-10.
[7] Lützow M V, Kögel-Knabner I, Ekschmitt K, et al. Stabilization of organic matter in temperate soils: mechanisms and their relevance under different soil conditions: a review[J]. European Journal of Soil Science, 2006, 57(4): 426-445. DOI: 10.1111/j.1365-2389.2006.00809.x.
[8] 何冬梅, 王火, 祝亚云, 等. 盐城滨海湿地土壤溶解性有机质的光谱特征分析[J]. 生态与农村环境学报, 2023, 39(6): 781-787. DOI: 10.19741/j.issn.1673-4831.2022.0424.
[9] 石媛媛, 赵隽宇, 宋贤冲, 等. 广西人工林不同类型土壤肥力质量评价及环境驱动因子分析[J]. 广西师范大学学报(自然科学版), 2023, 41(6): 192-201. DOI: 10.16088/j.issn.1001-6600.2022112301.
[10] 梁栋, 周巧林, 张辉, 等. 生物质炭和有机肥配施对水稻土溶解性有机质光谱学特征的影响[J]. 土壤学报, 2024, 61(4): 1123-1133. DOI: 10.11766/trxb202302020039.
[11] Ikeya K, Hikeye T, Arai S, et al. Size distribution of condensed aromatic rings in various humic acids[J]. Organic Geochemistry, 2011, 42(1): 55-61. DOI: 10.1016/j.orggechem.2010.10.006.
[12] 杭子清, 王国祥, 刘金娥, 等. 互花米草盐沼土壤有机碳库组分及结构特征[J]. 生态学报, 2014, 34(15): 4175-4182. DOI: 10.5846/stxb201212141802.
[13] 陈旭, 韩晓增, 严君, 等. 基于傅里叶变换离子回旋共振质谱测定的土壤溶解性有机质研究进展[J]. 土壤通报, 2019, 50(3): 732-738. DOI: 10.19336/j.cnki.trtb.2019.03.32.
[14] 龙云川, 蒋娟, 胡菁, 等. 高原湿地草海不同覆被土壤中溶解性有机质光谱特征[J]. 环境科学研究, 2025, 38(4): 896-906. DOI: 10.13198/j.issn.1001-6929.2024.12.22.
[15] 张慧颖. 低分子量有机酸介导生物炭释放磷和钾的机制研究[D]. 福州: 福建师范大学, 2021.
[16] 李丰义. 低分子量有机酸对土壤氧化铁形成与稳定性的影响[D]. 武汉: 华中农业大学, 2017.
[17] 王永壮, 陈欣, 史奕, 等. 低分子量有机酸对土壤磷活化及其机制研究进展[J]. 生态学杂志, 2018, 37(7): 2189-2198. DOI: 10.13292/j.1000-4890.201807.035.
[18] Lundström U, Öhman L O. Dissolution of feldspars in the presence of natural, organic solutes[J]. Journal of Soil Science, 1990, 41(3): 359-369. DOI: 10.1111/j.1365-2389.1990.tb00071.x.
[19] 孔涛, 刘民, 淑敏, 等. 低分子量有机酸对土壤微生物数量和酶活性的影响[J]. 环境化学, 2016, 35(2): 348-354.
[20] 徐仁扣. 有机酸对酸性土壤中铝的溶出和铝离子形态分布的影响[J]. 土壤, 1998, 30(4): 214-217. DOI: 10.13758/j.cnki.tr.1998.04.009.
[21] 盛浩, 宋迪思, 周萍, 等. 土地利用变化对花岗岩红壤底土溶解性有机质数量和光谱特征的影响[J]. 生态学报, 2017, 37(14): 4676-4685.
[22] 田翔, 何天容, 尹德良, 等. 土壤溶解性有机质结构和组成对秸秆、牛粪及其堆肥产品输入的响应特征[J]. 农业资源与环境学报, 2022, 39(3): 556-566. DOI: 10.13254/j.jare.2021.0068.
[23] 李海波, 赵孝明, 李英华, 等. 黄菖蒲根际低分子量有机酸对土壤吸附氨氮的影响[J]. 东北大学学报(自然科学版), 2020, 41(2): 269-274. DOI: 10.12068/j.issn.1005-3026.2020.02.021.
[24] 曹贤发, 李海玲. 中国典型岩溶发育区域划分[J]. 地球科学前沿, 2018, 8(4): 845-851.DOI: 10.12677/AG.2018.84092.
[25] 孙艳丽, 况明生, 张远嘱, 等. 中国南方岩溶地区脆弱的生态环境及石漠化过程[J]. 贵州师范大学学报(自然科学版), 2003, 21(2): 80-83. DOI: 10.16614/j.cnki.issn1004-5570.2003.02.022.
[26] 文冬妮. 西南岩溶石漠化区土地退化过程中土壤氮转化特征及调控[D]. 海口: 海南大学, 2024.
[27] 周游游. 农业活动与岩溶山区土地利用的可持续性探讨[J]. 地理科学, 2000, 20(5): 431-436. DOI: 10.13249/j.cnki.sgs.2000.05.008.
[28] 周建超. 中、晚全新世气候、沉积环境变化在桂林岩溶湿地沉积物中的记录[D]. 重庆: 西南大学, 2015.
[29] 骆东奇, 白洁, 谢德体. 论土壤肥力评价指标和方法[J]. 土壤与环境, 2002, 11(2): 202-205. DOI: 10.3969/j.issn.1674-5906.2002.02.020.
[30] 全国土壤普查办公室. 中国土壤[M]. 北京: 中国农业出版社, 1998.
[31] Tivet F, De Moraes S J C, Lal R, et al. Assessing humification and organic C compounds by laser-induced fluorescence and FTIR spectroscopies under conventional and no-till management in Brazilian Oxisols[J]. Geoderma, 2013, 207: 71-81. DOI: 10.1016/j.geoderma.2013.05.001.
[32] Ibarra J, Moliner R, Bonet A J. FT-IR. investigation on char formation during the early stages of coal pyrolysis[J]. Fuel, 1994, 73(6): 918-924. DOI: 10.1016/0016-2361(94)90287-9.
[33] Ibarra J, Muñoz E, Moliner R. FTIR study of the evolution of coal structure during the coalification process[J]. Organic Geochemistry, 1996, 24(6/7): 725-735. DOI: 10.1016/0146-6380(96)00063-0.
[34] 孙宝利, 赤杰, 范中南, 等. 土壤及植物复合体系中有机酸的测定[J]. 环境科学与技术, 2010, 33(9): 130-134. DOI: 10.3969/j.issn.1003-6504.2010.09.027.
[35] Minasny B, Mcbratney A B, Wadoux A M J C, et al. Precocious 19th century soil carbon science[J]. Geoderma Regional, 2020, 22: e00306. DOI: 10.1016/j.geodrs.2020.e00306.
[36] Pan Y H, Li M W, Sun Y G, et al. Characterization of free and bound bitumen fractions in a thermal maturation shale sequence. Part 2: structural evolution of kerogen and bitumen during shale oil generation, expulsion and retention[J]. Organic Geochemistry, 2023, 182: 104640. DOI: 10.1016/j.orggeochem.2023.104640.
[37] 王擎, 张岩, 迟铭书. 干酪根的热解特性及脂肪链含量的测定[J]. 石油学报(石油加工), 2017, 33(4): 771-776. DOI: 10.3969/j.issn.1001-8719.2017.04.023.
[38] 李岩, 王云鹏, 赵长毅, 等. 煤中干酪根在热演化中结构变化的红外光谱研究[J]. 矿物岩石地球化学通报, 2013, 32(1): 97-101, 106.
[39] 郭隽虹, 邹艳荣, 颜永何, 等. 干酪根分子结构在低熟阶段的演化特征: 基于红外光谱分析[J]. 地球化学, 2014, 43(5): 529-537. DOI: 10.19700/j.0379-1726.2014.05.011.
[40] Ashraf M N, Hu C, Xu X R, et al. Long-term manure application increased soil organic carbon and nitrogen mineralization through accumulation of unprotected and physically protected carbon fractions[J]. Pedosphere, 2023, 33(2): 343-354. DOI: 10.1016/j.pedsph.2022.06.047.
[41] 林小兵, 孙永明, 江新凤, 等. 江西省茶园土壤肥力特征及其影响因子[J]. 应用生态学报, 2020, 31(4): 1163-1174. DOI: 10.13287/j.1001-9332.202004.022.
[42] Liptzin D, Silver W L, Detto M. Temporal dynamics in soil oxygen and greenhouse gases in two humid tropical forests[J]. Ecosystems, 2011, 14(2): 171-182. DOI: 10.1007/s10021-010-9402-x.
[43] Gerasimowicz W V, Byler D M. Carbon-13 CPMAS NMR and FTIR spectroscopic-studies of humic acids[J]. Soil Science, 1985, 139(3): 270-278. DOI: 10.1097/00010694-198503000-00013.
[44] 梁尧. 有机培肥对黑土有机质消长及其组分与结构的影响[D]. 哈尔滨: 中国科学院研究生院(东北地理与农业生态研究所), 2012.
[45] Rugova A, Puschenreiter M, Koellensperger G, et al. Elucidating rhizosphere processes by mass spectrometry: a review[J]. Analytica Chimica Acta, 2017, 956: 1-13. DOI: 10.1016/j.aca.2016.12.044.
[46] Kögel-Knabner I. The macromolecular organic composition of plant and microbial residues as inputs to soil organic matter[J]. Soil Biology and Biochemistry, 2002, 34(2): 139-162. DOI: 10.1016/S0038-0717(01)00158-4.
[47] 王齐磊, 江韬, 赵铮, 等. 三峡库区典型农业小流域土壤溶解性有机质的紫外-可见及荧光特征[J]. 环境科学, 2015, 36(3): 879-887. DOI: 10.13227/j.hjkx.2015.03.017.
[48] 李晖, 尹辉, 蒋忠诚, 等. 典型岩溶区石漠化和土壤侵蚀遥感解译与关键问题[J]. 广西师范大学学报(自然科学版), 2013, 31(2): 133-139. DOI: 10.16088/j.issn.1001-6600.2013.02.012.
[49] 韦复才, 时坚, 唐建生, 等. 西南岩溶区生态退化研究[J]. 广西师范大学学报(自然科学版), 2006, 24(2): 101-105. DOI: 10.16088/j.issn.1001-6600.2006.02.025.
[50] 张福韬, 乔云发, 苗淑杰, 等. 长期玉米连作下黑土各组分有机质化学结构特征[J]. 中国农业科学, 2016, 49(10): 1913-1924. DOI: 10.3864/j.issn.0578-1752.2016.10.008.
[51] 白莹. 白云岩地区生态恢复的土壤有色溶解有机质(CDOM)响应过程及土壤质量评价[D]. 重庆: 西南大学, 2022.
[52] 郝翔翔, 韩晓增, 邹文秀. 示差红外光谱在土壤有机质组成研究中的应用[J]. 分析化学, 2018, 46(4): 616-622. DOI: 10.11895/j.issn.0253-3820.171100.
[53] Lee Y K, Lee M H, Hur J. A new molecular weight (MW) descriptor of dissolved organic matter to represent the MW-dependent distribution of aromatic condensation: insights from biodegradation and pyrene binding experiments[J]. Science of the Total Environment, 2019, 660: 169-176. DOI: 10.1016/j.scitotenv.2019.01.035.
[54] Olofsdotter M, Jensen L B, Courtois B. Improving crop competitive ability using allelopathy: an example from rice[J]. Plant Breeding, 2002, 121(1): 1-9. DOI: 10.1046/j.1439-0523.2002.00662.x.
[55] Van Hees P A W, Jones D L, Godbold D L. Biodegradation of low molecular weight organic acids in coniferous forest podzolic soils[J]. Soil Biology and Biochemistry, 2002, 34(9): 1261-1272. DOI: 10.1016/S0038-0717(02)00068-8.
[56] Van Hees P A W, Dahlén J, Lundström U S, et al. Determination of low molecular weight organic acids in soil solution by HPLC[J]. Talanta, 1999, 48(1): 173-179. DOI: 10.1016/S0039-9140(98)00236-7.
[57] Taghipour M, Jalali M. Effect of low-molecular-weight organic acids on kinetics release and fractionation of phosphorus in some calcareous soils of western Iran[J]. Environmental Monitoring and Assessment, 2013, 185(7): 5471-5482. DOI: 10.1007/s10661-012-2960-y.
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