Journal of Guangxi Normal University(Natural Science Edition) ›› 2016, Vol. 34 ›› Issue (4): 121-128.doi: doi:10.16088/j.issn.1001-6600.2016.04.018

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Isolation and Identification of a Strain Capable of Transforming trans-Anethole to Anisic Acid

WANG Xinyan1,2, SHEN Peihong1,2, HUA Yanfei1,2, LI Junfang1, ZHANG Min1, WU Bo2,3   

  1. 1.College of Life Science and Technology, Guangxi University, Nanning Guangxi 530003, China;
    2.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning Guangxi 530005, China;
    3.College of Biology and Food Engineering, Guangxi Normal University for Nationalities, Chongzuo Guangxi 532200, China
  • Online:2016-07-18 Published:2018-07-23

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Abstract: After the gradient acclimation and enrichment screening, 348 strains were isolated from environmental samples using trans-anethole as the sole carbon source. Through quantitative detection of trans-anethole and anisic acid with the methods of TLC and RP-HPLC, a strain named WGBF9 with the highest tolerance to trans-anethole and maximum yield of anisic acid was obtained. Biomass of strain WGBF9 was 37.42% of the blank control when the seed culture medium contained 10% (v/v) trans-anethole, and the biomass reached 26.57% in the medium with 20% (v/v) trans-anethole, showing that the strain had a high tolerance to the trans-anethole. Anisic acid molar generation rate is 17.9%, when the strain was cultured in the screening secondary medium, with rotate speed of 200 r/min, after 36 h fermentation under 30 ℃. WGBF9 was identified as a subspecies of Pseudomonas putida biotype A based on the analysis of physiological and biochemical experiments.

Key words: Pseudomonas putida, trans-anethole, anisic acid

CLC Number: 

  • Q93
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