Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (4): 165-177.doi: 10.16088/j.issn.1001-6600.2022072301

Previous Articles     Next Articles

Antioxidant Activity and Chemical Composition Analysis of Zhuang Medicine Stahlianthus involucratus

LIANG Jie1,2,3,4, LIU Xingchen1,2,3,4, SUN Zhengyi5, CHEN Xiaosi1,2,3,4, YANG Chuanchuan1,2,3,4, CAO Yupin1,3, LI Yaohua1,3, CHEN Mingwei1*, HUANG Tuanxin6*   

  1. 1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning Guangxi 530200, China;
    2. South China Branch of National Engineering Research Center for Manufacturing Technology of Traditional Chinese Medicine Solid Preparation (Guangxi University of Chinese Medicine), Nanning Guangxi 530200, China;
    3. Key Laboratory of TCM Extraction and Purification and Quality Analysis (Guangxi University of Chinese Medicine), Nanning Guangxi 530200, China;
    4. Education Department of Guangxi Zhuang Autonomous Region, Guangxi Zhuang Yao Medicine Center of Engineering and Technology (Guangxi University of Chinese Medicine), Nanning Guangxi 530200, China;
    5. Department of Pharmacy, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning Guangxi 530011, China;
    6. Guangxi Institute for Food and Drug Control, Nanning Guangxi 530021, China
  • Received:2022-07-23 Revised:2022-09-06 Online:2023-07-25 Published:2023-09-06

Abstract: In order to study the antioxidant activity and composition of Zhuang medicine Stahlianthus involucratus, the antioxidant capacity and chemical composition of Stahlianthus involucratus were analyzed through DPPH、O2-· and ·OH free radical scavenging experiments and UPLC-Q-Orbitrap HRMS. The results showed that different extracts of Stahlianthus involucratus had certain antioxidant effect, and the ethyl acetate extracts had the strongest antioxidant ability. In total, 14 compounds were identified from Stahlianthus involucratus for the first time, including curcumol, curdione, α-cyperone, azelaic acid, citric acid, protocatechuic aldehyde, isoschaftoside, uridine, L-tyrosine, nicotinamide, L-tryptophan, mannitol and 3-butylidenephthalide. There is a certain antioxidant capacity in the Stahlianthus involucratus, protocatechuic aldehyde, nicotinamide and other components may be the substances that directly play the key role. This study provides a reference for the development and utilization of Stahlianthus involucratus.

Key words: Stahlianthus involucratus, antioxidant, UPLC-Q-Orbitrap HRMS, chemical composition

CLC Number:  R285
[1] 广西壮族自治区食品药品监督管理局. 广西壮族自治区壮药质量标准:第2卷[M]. 南宁: 广西科学技术出版社, 2011: 12.
[2] 中国科学院中国植物志编辑委员会. 中国植物志:第16卷[M]. 北京: 科学出版社, 1981: 44.
[3] 胡稀, 谢珍珍, 梁洁. 壮药姜叶三七醇提取物抗炎作用的研究[J]. 大众科技, 2017, 19(1): 37-38. DOI: 10.3969/j.issn.1008-1151.2017.01.015.
[4] 于政, 郑雪绒, 赵亚宁, 等. 姜三七对子宫肌瘤大鼠子宫组织细胞凋亡及Bcl-2、Bax蛋白表达的影响[J]. 现代生物医学进展, 2017, 17(35): 6921-6924, 6991. DOI: 10.13241/j.cnki.pmb.2017.35.028.
[5] 许静, 杨柳, 聂伟. 姜三七对子宫肌瘤患者血清中Bcl-2和Bax水平影响[J]. 中国生化药物杂志, 2015, 35(5): 118-120.
[6] 王蔓莉, 邓立东, 徐勤. 三七姜挥发油诱导(CNE-2)细胞周期G1期阻滞及相关基因表达的影响[J]. 中国医院药学杂志, 2015, 35(5): 409-413. DOI: 10.13286/j.cnki.chinhosppharmacyj.2015.05.10.
[7] 梁洁, 陈辉华, 杨川川, 等. 壮药三七姜乙酸乙酯提取物抗炎、镇痛作用的谱-效关系研究[J]. 中国药房, 2021, 32(14): 1721-1727. DOI: 10.6039/j.issn.1001-0408.2021.14.10.
[8] 邹碧群, 张业. 吴茱萸、桑葚、柚子和香蕉抗氧化活性研究[J]. 广西师范大学学报(自然科学版), 2016, 34(4): 50-54. DOI: 10.16088/j.issn.1001-6600.2016.04.008.
[9] 李伶, 唐文迪, 陈寿涣, 等. 岩黄连总生物碱的提取工艺及抗氧化活性研究[J]. 广西师范大学学报(自然科学版), 2022, 40(6): 145-153. DOI: 10.16088/j.issn.1001-6600.2021093003.
[10] 李丹倩, 梁嘉怡, 钟晓晴, 等. 火龙果皮发酵物对脂多糖诱导RAW264.7细胞炎症的缓解作用[J]. 现代食品科技, 2021, 37(5): 23-30, 16. DOI: 10.13982/j.mfst.1673-9078.2021.5.0999.
[11] 王潞, 周云英. 杨梅素抗感染、抗炎及抗氧化活性研究进展[J]. 中草药, 2019, 50(3): 778-784. DOI: 10.7501/j.issn.0253-2670.2019.03.035.
[12] 谢珍珍, 杨颖欣, 梁洁, 等. 壮药姜叶三七的化学成分鉴别研究[J]. 广西中医药大学学报, 2015, 18(3): 49-51.
[13] 王荣, 孙艳平, 杨宽, 等. DPPH和T-AOC测定法评价黄芪桂枝配伍的抗氧化能力研究[J]. 中医药导报, 2018, 24(23): 63-64, 69. DOI: 10.13862/j.cnki.cn43-1446/r.2018.23.019.
[14] 王锦霞, 郭萌萌, 刘亚昕, 等. 红甜菜提取物及甜菜红素的体外抗氧化活性分析[J]. 中国糖料, 2021, 43(4): 59-63. DOI: 10.13570/j.cnki.scc.2021.04.010.
[15] 蒋边, 李卫锦, 肖理峰, 等. 杨桃多酚提取液体外抗氧化活性研究[J]. 食品研究与开发, 2020, 41(24): 50-56. DOI: 10.12161/j.issn.1005-6521.2020.24.009.
[16] 梁洁, 韦金玉, 黄冬芳, 等. 基于UPLC-Q-TOF-MS/MS技术的龙眼叶入血成分分析[J].中药材, 2021, 44(3): 617-623. DOI: 10.13863/j.issn1001-4454.2021.03.021.
[17] 朱圣军, 李俊, 蒙爱萍, 等. 鹊肾树叶提取物抗氧化活性研究[J]. 广西师范大学学报(自然科学版), 2010, 28(3): 33-36. DOI: 10.16088/j.issn.1001-6600.2010.03.016.
[18] 麦嘉妮, 梁洁, 徐晖, 等. 龙眼叶抗氧化活性部位的血清药物化学初步研究[J]. 时珍国医国药, 2018, 29(12): 2817-2821. DOI: 10.3969/j.issn.1008-0805.2018.12.001.
[19] 吕狄亚. 中药莪术化学成分分析及药代动力学研究[D]. 上海:第二军医大学, 2011. DOI: 10.7666/d.d151458.
[20] 孙燕, 冯峰, 黄特辉, 等. 基于UPLC-Q-Exactive技术结合OTCML数据库快速分析沙棘的功效成分[J]. 天然产物研究与开发, 2019, 31(7): 1192-1202. DOI: 10.16333/j.1001-6880.2019.7.011.
[21] GUO L, GONG M X, WU S, et al. Identification and quantification of the quality markers and anti-migraine active components in Chuanxiong Rhizoma and Cyperi Rhizoma herbal pair based on chemometric analysis between chemical constituents and pharmacological effects[J]. Journal of Ethnopharmacology, 2020, 246: 112228. DOI: 10.1016/j.jep.2019.112228.
[22] JIANG M, ZHOU M G, HAN Y Q, et al. Identification of NF-κB inhibitors in Xuebijing injection for sepsis treatment based on bioactivity-integrated UPLC-Q/TOF[J]. Journal of Ethnopharmacology, 2013, 147(2): 426-433. DOI: 10.1016/j.jep.2013.03.032.
[23] SUN D X, DONG L H, GUO P P, et al. Simultaneous detection of flavonoids and phenolic acids in Herba Lysimachiae and Herba Desmodii Styracifolii using liquid chromatography tandem mass spectrometry[J]. Food Chemistry, 2013, 138(1): 139-147. DOI: 10.1016/j.foodchem.2012.09.096.
[24] XUE Y, JIN W, XU X S, et al. Quality evaluation of Tricholoma matsutake based on the nucleic acid compounds by UPLC-TOF/MS and UPLC-QqQ/MS[J]. Molecules, 2018, 24(1): 34-44. DOI: 10.3390/molecules24010034.
[25] REIS LIMA M J, SANTOS A O, FALCÃO S, et al. Serra da Estrela cheese’s free amino acids profiles by UPLC-DAD-MS/MS and their application for cheese origin assessment[J]. Food Research International, 2019, 126: 108729. DOI: 10.1016/j.foodres.2019.108729.
[26] INAMADUGU J K, DAMARAMADUGU R, MULLANGI R, et al. Simultaneous determination of niacin and its metabolites-nicotinamide, nicotinuric acid and N-methyl-2-pyridone-5-carboxamide-in human plasma by LC-MS/MS and its application to a human pharmacokinetic study[J]. Biomedical Chromatography, 2010, 24(10): 1059-1074. DOI: 10.1002/bmc.1406.
[27] FU C Y, WU Q, ZHANG Z M, et al. UPLC-ESI-IT-TOF-MS metabolomic study of the therapeutic effect of Xuefu Zhuyu decoction on rats with traumatic brain injury[J]. Journal of Ethnopharmacology, 2019, 245: 112149. DOI: 10.1016/j.jep.2019.112149.
[28] CMARA NETO J F, DA SILVA CAMPELO M, CERQUEIRA G S, et al. Gastroprotective effect of hydroalcoholic extract from Agaricus blazei Murill against ethanol-induced gastric ulcer in mice[J]. Journal of Ethnopharmacology, 2022, 292: 115191. DOI: 10.1016/j.jep.2022.115191.
[29] ZHAO Y, SUN J H, YU L L, et al. Chromatographic and mass spectrometric fingerprinting analyses of Angelica sinensis(Oliv.) diels-derived dietary supplements[J]. Analytical and Bioanalytical Chemistry, 2013, 405(13): 4477-4485. DOI: 10.1007/s00216-012-6668-1.
[30] LU G H, CHAN K, LIANG Y Z, et al. Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs[J]. Journal of Chromatography A, 2005, 1073(1/2): 383-392. DOI: 10.1016/j.chroma.2004.11.080.
[31] DAWIDOEICZ A L, WIANOWSKA D, OLSZOWY M, et al. On practical problems in estimation of antioxidant activity of compounds by DPPH method (problems in estimation of antioxidant activity)[J]. Food Chemistry, 2012, 131(3): 1037-1043. DOI: 10.1016/j.foodchem.2011.09.067.
[32] SHARMA O P, BHAT T K. DPPH antioxidant assay revisited[J]. Food Chemistry, 2009, 113(4): 1202-1205. DOI: 10.1016/j.foodchem.2008.08.008.
[33] KEDARE S B, SINGH R P. Genesis and development of DPPH method of antioxidant assay[J]. Journal of Food Science and Technology, 2011, 48(4): 412-422. DOI: 10.1007/s13197-011-0251-1.
[34] 李若飞, 于春萍, 兰丁璇, 等. 中药血清药理学实验方法与相关问题探讨[J]. 全科口腔医学电子杂志, 2019, 6(31): 125, 136. DOI: 10.16269/j.cnki.cn11-9337/r.2019.31.090.
[35] 武晓伟, 郝艳艳, 聂春霞, 等. 中药血清药物化学的方法学概述及应用领域研究进展[J].中国实验方剂学杂志, 2019, 25(3): 173-179. DOI: 10.13422/j.cnki.syfjx.20182003.
[36] 熊皓平, 杨伟丽, 张友胜, 等. 天然植物抗氧化剂的研究进展[J]. 天然产物研究与开发, 2001, 13(5): 75-79. DOI: 10.16333/j.1001-6880.2001.05.022.
[37] 张囡, 杜丽丽, 王冬, 等. 中药酚酸类成分的研究进展[J]. 中国现代中药, 2006, 8(2): 25-28. DOI: 10.3969/j.issn.1673-4890.2006.02.010.
[38] 乔丽萍, 傅瑜, 叶兴乾, 等. 酚酸生物活性研究进展[J]. 中国食品学报, 2013, 13(10): 144-152. DOI: 10.16429/j.1009-7848.2013.10.032.
[39] 石红, 石磊, 李建雄. 莪术二酮对肝部分切除老年小鼠术后认知功能和海马区炎性因子表达的影响[J]. 西安交通大学学报(医学版), 2017, 38(5): 749-752. DOI: 10.7652/jdyxb201705026.
[40] 黄菊阳, 王昭昕, 王少侠, 等. 醒脑静注射液活性成分对氧糖剥夺/复氧损伤的PC12细胞的保护作用研究[J]. 中国临床药理学杂志, 2020, 36(10): 1337-1340. DOI: 10.13699/j.cnki.1001-6821.2020.10.045.
[41] ZHANG S J, GAI Z B, GUI T, et al. Antioxidant effects of protocatechuic acid and protocatechuic aldehyde: old wine in a new bottle[J]. Evidence-based Complement Alternative Medicine, 2021, 2021: 6139308. DOI: 10.1155/2021/6139308.
[42] GUO C, WANG S Q, DUAN J L, et al. Protocatechualdehyde protects against cerebral ischemia-reperfusion-induced oxidative injury via protein kinase Cε/Nrf2/HO-1 pathway[J]. Molecular Microbiology, 2017, 54(2): 833-845. DOI: 10.1007/s12035-016-9690-z.
[43] 杨驰, 郑咏秋, 戴敏. 烟酰胺药理作用研究进展[J]. 临床肺科杂志, 2011, 16(12): 1914-1916. DOI: 10.3969/j.issn.1009-6663.2011.12.044.
[1] YAN Mengqi, WEI Yishen, HUANG Wanyi, WANG Lisheng, QIN Jiangke, ZHOU Xianli, LIANG Chengqin. Chemical Constituents of Stachys geobombycis C. Y. Wu [J]. Journal of Guangxi Normal University(Natural Science Edition), 2023, 41(2): 124-130.
[2] LI Ling, TANG Wendi, CHEN Shouhuan, NING Yulong, QIN Jiangke. Study on Extraction Technology and Antioxidant Activity of Total Alkaloids from Corydalis saxicola Bunting [J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(6): 145-153.
[3] CHEN Xu, LIANG Chengqin. Spectral Features, α-glucosidase Inhibitory Activities, Antioxidant Activities and Quantitative Determination of Mogroside ⅡA of Siraitia grosvenorii [J]. Journal of Guangxi Normal University(Natural Science Edition), 2019, 37(3): 132-141.
[4] ZOU Biqun. Antioxidant Activity of Vodiamine, Mulberry, Pomelo and Banana [J]. Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(4): 50-54.
[5] LIU Xi. Antioxidant and Antitumor Activities of Extracts from Taxus chinensis var. mairei [J]. Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(4): 55-59.
[6] ZHOU Jiang-xi, LIAO Lei, WU Ji-yu, CAI Jian-heng, WEI Su-na. Treatment of Washing Cooking Fumes and Analysis on Main Pollutant Composition in Cooking Fumes [J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(3): 79-85.
[7] GUO Yan-ju, PENG Feng-lin. Effects of Flavones on Antioxidant Enzymes and Apoptosis in Heart of Exhauted Exercise Rats [J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(2): 143-148.
[8] YI Li-na, BAI Kun-dong, LI Ming-xia, SHI Gui-yu. Comparison of Physiology and Biochemical Index of Some EvergreenandDeciduous Broad-leaved Species in Mao'er Mountain,Guangxi,China [J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(1): 83-88.
[9] YIN Wen-qing, DUAN Shao-qing, ZHANG Yan, ZENG Li, SONG Xin-ming. Antioxidant Activities of Different Solvents Extracts and Alkaloids of Uncaria rhynchophylla (Miq.)Jacks [J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(1): 31-34.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] XU Jiu-cheng, LI Xiao-yan, LI Shuang-qun, ZHANG Ling-jun. Feature Images Retrieval Method of Tolerance Granular-basedMulti-level Texture[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(1): 186 -187 .
[2] BAI Defa, XU Xin, WANG Guochang. Review of Generalized Linear Models and Classification for Functional Data[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(1): 15 -29 .
[3] ZENG Qingfan, QIN Yongsong, LI Yufang. Empirical Likelihood Inference for a Class of Spatial Panel Data Models[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(1): 30 -42 .
[4] ZHANG Xilong, HAN Meng, CHEN Zhiqiang, WU Hongxin, LI Muhang. Survey of Ensemble Classification Methods for Complex Data Stream[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 1 -21 .
[5] TONG Lingchen, LI Qiang, YUE Pengpeng. Research Progress and Prospects of Karst Soil Organic Carbon Based on CiteSpace[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 22 -34 .
[6] WANG Dangshu, YI Jiaan, DONG Zhen, YANG Yaqiang, DENG Xuan. Research on Bridgeless Boost PFC Converter with Ripple Suppression Unit Based on Single Cycle Control[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 47 -57 .
[7] YU Siting, PENG Jingjing, PENG Zhenyun. Rank Constraint Least Square Symmetric Semidefinite Solutions and Its Optimal Approximation of the Matrix Equation[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 136 -144 .
[8] QIN Chengfu, MO Fenmei. Structure ofC3-and C4-Critical Graphs[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 145 -153 .
[9] YIN Yudong, KE Shanzhe, HUANG Jiayan, DENG Mengxiang, LIU Guanyan, CHENG Keguang. One-pot Generation of Allylated Products from Alcohols, Carboxylic Acids and Amines with 1,3-Dibromopropane by Sodium Hydride[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 154 -161 .
[10] DU Libo, LI Jinyu, ZHANG Xiao, LI Yonghong, PAN Weidong. Chemical Constituents and Biological Activity from the Bark of Toona ciliata var. pubescens[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 162 -172 .