Journal of Guangxi Normal University(Natural Science Edition) ›› 2022, Vol. 40 ›› Issue (6): 206-214.doi: 10.16088/j.issn.1001-6600.2021072804

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Genetic Diversity Analysis Among 5 Cultured Populations ofQuasipaa spinosa by Microsatellite Markers

WEI Zhaoyu1,2,3, WANG Xiaodong1,2,4, WEI Xiuying1,2,3, YUAN Hong1,2,3, YAO Hongyan1,2,3, CHEN Dunxue1,2,3*   

  1. 1. College of Animal Science, Guizhou University, Guiyang Guizhou 550025, China;
    2. Research Center of Fishery Resources and Environment, Guizhou University, Guiyang Guizhou 550025, China;
    3. Key Laboratory of Animal Genetics and Breeding and Reproduction of Plateau and Mountain Animals (Guizhou University), Guiyang Guizhou 550025, China;
    4. College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China
  • Received:2021-07-28 Revised:2021-08-26 Online:2022-11-25 Published:2023-01-17

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Abstract: In order to explore the genetic diversity and genetic status of the Quasipaa spinosa, 9 highly polymorphic microsatellite makers were chosen to comprehend the genetic information based on 148 samples from five breeding populations (Jiangxi, Zhejiang, Guangxi, Guangdong and Fujian population). A total of 46 different alleles were detected, with mean number of alleles and effective alleles were 5.111 and 2.702, respectively. The observed mean heterozygosity was 0.182, and the expected heterozygosity was 0.589 while the polymorphic information content reached to 0.536. Among populations, the heterozygosity and expected heterozygosity were ranged from 0.130 to 0.230 and 0.353 to 0.468, respectively, with the heterozygosity lesser than the expected heterozygosity. The genetic relationship of Guangdong and Guangxi population showed the highest genetic similarity (0.819), while Fujian and Jiangxi population showed the lowest genetic similarity (0.441), according to the reslut based on UPGMA clustering. The study indicates that the cultured population of Q. spinosa has a degree inbreeding, suggesting that the breeding range should be expanded and superior varieties from other fields should be introduced regularly as breeding parents to reduce inbreeding.

Key words: Quasipaa spinosa, culture populations, microsatellite marker, genetic diversity, genetic structure

CLC Number: 

  • Q959.4
[1] HU W F, DONG B J, KONG S S, et al. Pathogen resistance and gene frequency stability of major histocompatibility complex class IIB alleles in the giant spiny frog Quasipaa spinosa[J]. Aquaculture,2017, 468(Part 1): 410-416. DOI: 10.1016/j.aquaculture.2016.11.001.
[2] CHAN H K, SHOEMAKER K T, KARRAKER N E. Demography of Quasipaa frogs in China reveals high vulnerability to widespread harvest pressure[J]. Biological Conservation, 2014, 170: 3-9. DOI: 10.1016/j.biocon.2013.12.014.
[3] SHEN B, CHEN P, ZHENG R Q, et al. Geographic variation in the advertisement calls of the giant spiny frog (Paa spinosa)[J]. Acta Ecologica Sinica, 2015, 35(1): 39-45. DOI: 10.1016/j.chnaes.2015. 01.001.
[4] 舒妙安. 棘胸蛙肌肉营养成分的分析(Ⅰ): 一般营养成分的含量及脂肪酸的组成[J].浙江大学学报(理学版), 2000, 34(4): 433-437. DOI: 10.3321/j.issn:1008-9497. 2000.04.16.
[5] 暨柳华. 棘胸蛙的生态习性与人工养殖方法研究[J].绿色科技,2018(14): 38-39. DOI: 10.16663/j.cnki.lskj.2018.14.013.
[6] 何南, 张小丽, 陈宁, 等. 基于SSR分析广东罗坑鳄蜥饲养种群的遗传结构[J]. 广西师范大学学报(自然科学版), 2022, 40(4): 180-187. DOI: 10.16088/j.issn.1001-6600.2021033101.
[7] 王丰, 张家华, 沈玉帮, 等. 青鱼野生与养殖群体遗传变异的微卫星分析[J]. 水生生物学报, 2019, 43(5): 939-944. DOI: 10.7541/2019.111.
[8] 肖起珍, 刘青, 吴旭干, 等. 长江水系中华绒螯蟹野生和人工繁殖大眼幼体的遗传多样性分析[J].基因组学与应用生物学, 2017, 36(5): 1935-1945. DOI: 10.13417/j.gab.036.001935.
[9] 郑翔, 徐杰杰, 张佳佳, 等. 4个瓦氏黄颡鱼群体遗传多样性的微卫星分析[J]. 水产科学, 2020,39(5): 657-668. DOI: 10.16378/j.cnki.1003-1111.2020.05.003.
[10] 刘南君, 吴太伦, 路健, 等. 宽阔水自然保护区棘胸蛙种群的RAPD遗传多样性[J]. 贵州农业科学, 2013, 41(1): 14-17. DOI: 10.3969/j.issn.1001-3601.2012.01.005.
[11] 王茂元,赖铭勇,黄洪贵,等.福建内陆山区棘胸蛙遗传多样性分析[J].水产学杂志,2020,33(5): 20-25. DOI: 10.3969/j.issn.1005-3832.2020.05.004.
[12] ZHENG R Q,YE R H,YU Y Y,et al.Fifteen polymorphic microsatellite markers for the giant spiny frog, Paa spinosa[J].Molecular Ecology Resources,2009,9(1): 336-338. DOI: 10.1111/j.1755-0998.2008. 02420.x.
[13] YE S P,HUANG H,ZHENG R Q,et al.Phylogeographic analyses strongly suggest cryptic speciation in the giant spiny frog(Dicroglossidae: Paa spinosa) and interspecies hybridization in Paa[J].PLoS One,2013,8(7): e70403. DOI: 10.1371/journal.pone.0070403.
[14] GAO Z X,LUO W,LIU H,et al.Transcriptome analysis and SSR/SNP markers information of the blunt snout bream (Megalobrama amblycephala)[J].PLoS One,2012,7(8): e42637. DOI: 10.1371/journal.pone.0042637.
[15] WANG Y F,LI C J,PAN C L,et al.Alterations to transcriptomic profile, histopathology, and oxidative stress in liver of pikeperch (Sander lucioperca) under heat stress[J].Fish & Shellfish Immunology,2019,95: 659-669. DOI: 10.1016/j.fsi.2019.11.014.
[16] ZHANG J,MA W G,SONG X M,et al.Characterization and development of EST-SSR markers derived from transcriptome of yellow catfish[J]. Molecules, 2014, 19(10): 16402-16415. DOI: 10.3390/molecules191016402.
[17] SOUZA-SHIBATTA L,KOTELOK-DINIZ T,FERREIRA D G,et al.Genetic diversity of the endangered neotropical cichlid fish (Gymnogeophagus setequedas) in Brazil[J]. Frontiers in Genetics,2018,9: 13. DOI: 10.3389/FGENE.2018.00013.
[18] 王沈同,沈玉帮,孟新展,等.草鱼野生与选育群体遗传变异微卫星分析[J].水产学报,2018,42(8): 1273-1284. DOI: 10.11964/jfc.20171010997.
[19] BOTSTEIN D,WHITE R L,SKOLNICK M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3): 314-331.PMID: 6247908.
[20] 魏朝宇, 谢永广, 魏秀英, 等. 基于转录组测序的棘胸蛙SSR和SNP分子标记开发[J]. 南方农业学报, 2022, 53(3): 759-767. DOI: 10.3969/j.issn.2095-1191.2022.03.017.
[21] SIMMONS M,MICKETT K,KUCUKTAS H,et al.Comparison of domestic and wild channel catfish (Ictalurus punctatus) populations provides no evidence for genetic impact[J].Aquaculture,2006,252(2/3/4): 133-146. DOI: 10.1016/j.aquaculture.2005.11.006.
[22] XU Z K,PRIMAVERA J H,PENA L D,et al.Genetic diversity of wild and cultured Black Tiger Shrimp (Penaeus monodon) in the Philippines using microsatellites[J].Aquaculture,2001,199(1/2): 13-40. DOI: 10.1016/S0044-8486(00)00535-4.
[23] 韩承慧,马海涛,姜海滨,等.许氏平鲉(Sebastes schlegeli)微卫星标记开发及野生、养殖群体遗传多样性分析[J].海洋与湖沼,2016,47(1): 213-220. DOI: 10.11693/hyhz20150900235.
[24] 甘宝江,张盛,韦玲静,等.广西稻田养殖金边鲤群体遗传多样性分析[J].水产科学,2019,38(5): 636-646. DOI: 10.16378/j.cnki.1003-1111.2019.05.008.
[25] QIN Y, SHI G, SUN Y. Evaluation of genetic diversity in Pampus argenteus using SSR markers[J]. Genetics and Molecular Research, 2013, 12(4): 5833-5841. DOI: 10.4238/2013.November.22.10.
[26] 崔蕾,谢从新,李艳和,等.斑点叉尾鮰4个群体遗传多样性的微卫星分析[J].华中农业大学学报,2012,31(6): 744-751. DOI: 10.13300/j.cnki.hnlkxb.2012.06.021.
[27] TAKEZAKI N,NEI M.Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA[J].Genetics,1996,144(1): 389-399. DOI: 10.1093/GENETICS/144.1.389.
[28] 刘慧芬,王静,黄建美,等.河南省鳙养殖群体的遗传多样性与选择压力分析[J].淡水渔业,2018,48(5): 22-29. DOI: 10.13721/j.cnki.dsyy.2018.05.004.
[29] 孙成飞,谢汶峰,胡婕,等.大口黑鲈3个养殖群体的遗传多样性分析[J].南方水产科学,2019,15(2): 64-71. DOI: 10.12131/20180203.
[30] 王长忠,李忠,梁宏伟,等.长江下游地区4个克氏原螯虾群体的遗传多样性分析[J]. 生物多样性,2009,17(5): 518-523. DOI: 10.3724/SP.J.1003.2009.09017.
[31] 何金钊,陈诏,陈子桂,等.五个红罗非鱼群体的遗传多样性分析[J].水生生物学报,2017,41(2): 326-333. DOI: 10.7541/2017.40.
[32] BALLOUX F, LUGON-MOULIN N. The estimation of population differentiation with microsatellite markers[J].Molecular Ecology,2002,11(2): 155-165. DOI: 10.1046/j.0962-1083.2001.01436.x.
[33] LEE W C.Searching for disease-susceptibility loci by testing for Hardy-Weinberg disequilibrium in a gene bank of affected individuals[J]. American Journal of Epidemiology,2003,158(5): 397-400. DOI: 10.1093/aje/kwg150.
[34] AUSTIN J J,OLIVIER L,NANKERVIS D,et al.Twenty microsatellite loci for population and conservation genetic studies of the wedge-tailed eagle (Aquila audax)[J]. Australian Journal of Zoology,2014,62(3): 235-237. DOI: 10.1071/ZO14030.
[35] 周梦晨,徐东坡,方弟安,等.6个不同群体鳜基于SSR的遗传多样性初步研究[J].中国农学通报,2020,36(26): 147-152.
[36] 李伟强,陈刚,马骞,等.利用微卫星标记分析军曹鱼养殖群体的遗传多样性[J].渔业科学进展,2020,41(2): 113-120. DOI: 10.19663/j.issn2095-9869.20190617001.
[37] 黄小芳, 唐章生, 刘俊丹, 等. 广西不同地区克氏原螯虾群体遗传多样性微卫星分析[J]. 南方农业学报, 2020, 51(2): 437-444. DOI: 10.3969/j.issn.2095-1191.2020.02.025.
[38] 韩斐斐,张继彪,李莉,等.虾夷扇贝养殖群体及其子代的遗传多样性分析[J].海洋科学,2012,36(9): 1-8.
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