Journal of Guangxi Normal University(Natural Science Edition) ›› 2022, Vol. 40 ›› Issue (4): 180-187.doi: 10.16088/j.issn.1001-6600.2021033101

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Genetic Structure of the Artificial Breeding Shinisaurus crocodilurus in Luokeng Nature Reserve Based on Microsatellite DNA Marker

HE Nan1,2, ZHANG Xiaoli1,3, CHEN Ning1,3, CHEN Zening1,3, WU Zhengjun1,3*   

  1. 1. Key Laboratory of Rare and Endangered Species and Environmental Protection (Guangxi Normal University),Ministry of Education, Guilin Guangxi 541006, China;
    2. Guangdong Qujiang Luokeng Shinisaurus crocodilurus Provincial Nature Reserve Management Office, Shaoguan Guangdong 512100, China;
    3. Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Guilin Guangxi 541006, China
  • Published:2022-08-05

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Abstract: Chinese crocodile lizard Shinisaurus crocodilurus is critically endangered with small population size. Although captive population is increasing, this species is still at risk of inbreeding due to their small population size. Therefore, it’s necessary to understand the genetic structure and relationship of this species to avoid inbreeding. Based on 9 pairs of microsatellite markers, the genetic structure of 112 S. crocodilurus in Luokeng Nature Reserve artificial breeding population was analyzed. The results indicated that: (1) Microsatellite loci detected a total of 106 alleles, with the average of 12 alleles for each loci. (2) Hardy-weinberg exact test are within the range of 0.314-0.998, the balance test coefficient is greater than 0.05. (3) The range of observed heterozygosity (Ho) is 0.018-0.929, with an average of 0.629, and the expected heterozygosity (He) ranges from 0.018 to 0.851, with an average of 0.622. (4) The individual Neighbor-joining tree of S. crocodilurus based on the Nei’s genetic distance showed that they clustered into two clades with three and five lineages in close genetic relationships. (5) Bottleneck effect detection results showed that under the TPM model, the captive population of S. crocodilurus from Luokeng Nature Reserve has experienced bottleneck effect in the recent history. These results indicated that the S. crocodilurus population in Luokeng Nature Reserve had an extremely low genetic diversity. It is recommended to consider genetical relationship in future artificial breeding to avoid inbreeding and keeping the genetic diversity.

Key words: Luokeng Nature Reserve, Shinisaurus crocodilurus, captive population, microsatellite, genetic structure

CLC Number: 

  • S865.3
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