闽楠幼苗对高温干旱复合胁迫的表型变化及其生理响应

doi:10.16088/j.issn.1001-6600.2025120102

Abstract

Abstract: Phoebe bournei is a precious subtropical tree species. However, the phenotypic and physiological response rules of its seedlings under high temperature, drought and their combined stress are still unclear, which restricts its cultivation regulation and germplasm utilization under extreme climates. One-year-old seedlings bred at Guanzhuang State-owned Forest Farm in Shaxian County, Fujian Province were used as the research objects. Three treatments were established in a phytotron, including 40 ℃ high temperature, 10% PEG-6000 simulated drought, and their combination, with normal growth conditions as the control. Phenotypic changes were recorded, and leaf biochemical indices including chlorophyll a, chlorophyll b, total chlorophyll, and malondialdehyde (MDA) were measured at 0, 6, 12, 24, 48, and 72 hours. The results showed that shoot tips yellowed and wilted at 12 hours. By 72 hours, stems turned brown and leaves became dry and brittle. Chlorophyll a showed the greatest decrease under combined stress. At 72 hours, chlorophyll a decreased by 36.49% compared with that of 0 hours and followed a “rise-first-then-fall” pattern. For membrane lipid peroxidation, MDA peaked at 24 hours under combined stress, increasing by 41.31% compared with that of 0 hours, and then dropped sharply, indicating acute injury followed by cell necrosis and abscission. The soluble protein content and the activities of SOD, POD and CAT all showed a trend of first increase and then decrease,and the peak value under combined stress were significantly higher than that under single stresses. Chlorophylls were significantly positively correlated with antioxidant enzymes and negatively correlated with MDA. The study indicates that,the damage of combined high temperature and drought stress to Phoebe bournei seedlings is significantly greater than that of single stress, which is mainly manifested by reactive oxygen species (ROS) burst, aggravated membrane lipid peroxidation and damage to the antioxidant system.

Key words: Phoebe bournei, heat stress, drought stress, combined stress, leaf phenotype, physiology

CLC Number: S792.24

Yang Hao, Liu Ronglin, Feng Yizhuo, Li Jingshu, Tang Xinghao, Cao Shijiang. Phenotypic changes and physiological responses of Phoebe bournei seedlings to combined heat and drought stress[J].Journal of Guangxi Normal University(Natural Science Edition), 2026, 44(5): 235-246.

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Phenotypic changes and physiological responses of Phoebe bournei seedlings to combined heat and drought stress

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