Journal of Guangxi Normal University(Natural Science Edition) ›› 2022, Vol. 40 ›› Issue (2): 231-241.doi: 10.16088/j.issn.1001-6600.2020112002

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Effects of Inoculation of AMF and Rhizobium on Photosynthetic and Respiratory Metabolism and Growth of Intercropping Glycine max

WU Yanfen1,2, LIU Qiuming1,2, LIU Weihuan1,2, MENG Aiping1,2, CHEN Zhenxiang1,2, LIU Ling1,2*   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Spices and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2020-11-20 Revised:2021-04-01 Published:2022-05-31

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Abstract: In order to explore the effects of inoculation of Glomus etunicatum (abbreviated as A, the similar as below) and Sinorhizobium fredii (R) on plant growth of soybean (Glycine max)in kumquat (Fortunella margarita (Lour.) Swingle (F)/soybean (S) intercropping system, a series of field experiments were conducted to analyze the photosynthetic and respiratory metabolism and plant growth of S inoculated with several treatments. The results showed that: 1)The mycorrhizal infection intensity ηm and vesicle intensity ηV in root system of S of all intercropping treatments were lower than those of monocropping treatments respectively, there were significant differences in ηm and no significant differences in ηV among the corresponding treatments (except that ηV increased significantly in intercropping double inoculation treatment compared with monoculture double inoculation treatment). Compared with the corresponding S or F+S treatment, those of all single inoculation A treatments or single inoculation R treatments showed significant or unsignificant promoting effects respectively, while the increase effect of ηm or ηV of double inoculation treatment A+R was the largest and the differences was significant respectively. 2)Compared with the corresponding monocropping treatments, the number of nodule and dry weight of S were decreased in intercropping system. Compared with the corresponding single inoculation A or R treatments, the promotion with A+R treatment were the most significant respectively. 3)The chlorophyll content and net photosynthetic rate of S were decreased with F+S treatment, while increased after inoculated with single A or R treatment. Those of the promotion effect of both A and R treatment was better than single A or R treatment. 4)The activity of succinate dehydrogenase of S in intercropping system were higher than that of monoculture system, and the promoting effect sequence of inoculation treatment was A+R>A>R. 5)The change trend of H+-ATPase activity in mitochondrial membrane of S leaves was similar to that of net photosynthetic rate. 6)The soybean biomass and yield of the intercropping inoculation treatment were higher than the monocropping inoculation treatment, and the inoculation treatments showed a decreasing trend of A+R>A>R. Therefore, treatment inoculated with both A and R could promote significantly the growth of S in intercropping system, the promotion effect was better than that of the single inoculation treatment.

Key words: arbuscular mycorrhizal fungi (Glomus etunicatum), rhizobium (Sinorhizobium fredii), intercropping soybean (Glycine max), photosynthetic physiology, respiratory metabolism

CLC Number: 

  • S565.1
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