青鳉nup58的表达及其在性别分化中的潜在作用

doi:10.16088/j.issn.1001-6600.2023012701

Abstract

Abstract: Nuclear pore complexes (NPCs) form channels to transport materials across the nuclear membrane. Nucleoporin can affect the material exchange between nucleoplasm and cytoplasm, and the nucleoplasm transport has the ability to coordinates germ cells into female or male differentiation pathways. Gonadal soma-derived factor (Gsdf) can regulate the male germ cells of Oryzias latipes and play a key role in switching on/off gametogenesis before the last turn of mitosis. The expression of nup58 (nucleoporin 58) was higher in normal testis than that in normal ovary detected by RT-qPCR. Moreover, compared with normal testicular transcriptome data of medaka(Oryzias latipes), the expression of nup58 in gsdf deficient testis was found to significantly increase by the RNA-seq. The results of immunofluorescence assay showed that nup58 was expressed in all germ cells at different stages of gametogenesis in both wild type and gsdf-knockout medaka gonads. This research shows that Nup58, as a nucleoporin, is regulated by gsdf signal and is closely related to the proliferation of male germ cells. Nup58 participates in dynamic events of nuclear cytoplasmic transport during sexual differentiation, and its synergistic effects need to be explored.

Key words: medaka, gonad, sex differentiation, transcriptome

CLC Number: Q786; S917

LI Yayuan, WANG Guangxing, LI Xinwen, GUO Zhenhua, GUAN Guijun. Expression of nup58 in Medaka and Its Potential Role in Sex Differentiation[J].Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(1): 168-179.

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Expression of nup58 in Medaka and Its Potential Role in Sex Differentiation

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