Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (6): 158-168.doi: 10.16088/j.issn.1001-6600.2022123001

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Effects of Ciprofloxacin on Operation Efficiency and Microbial Community Response Characteristics of Anaerobic Reactor for Treatment Phosphorus-Containing Wastewater

YANG Wenjing1, DENG Yulian1, CHEN Zhuxin1, TAO Afeng1, WEI Lixin1, WU Jinyan1, TIAN Yihao1, SU Chengyuan1,2*   

  1. 1. School of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China
  • Received:2022-12-30 Revised:2023-02-13 Published:2023-12-04

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Abstract: The presence of antibiotic pollutants in wastewater treatment process may affect the microbial activity in sludge, and the microbial activity directly affects the biological phosphorus removal effect of wastewater. Therefore, the effect of ciprofloxacin (CIP) on the operation efficiency of the anaerobic reactor treating high-concentration phosphorus wastewater and the response of microbial community were explored, and the COD removal rate, total phosphorus and phosphate removal rate, related enzyme activity and microbial community structure wereinvestigated. The results showed that the COD removal rate of high-concentration phosphorus wastewater in the reactor was stable at about 98% without CIP, and the average COD removal rate decreased to 63.1% when the CIP concentration was 10 mg/L, and the total phosphorus removal rate showed a downward trend in fluctuations. The addition of 10 mg/L CIP had a great effect on the concentration of phosphine; peaks associated with tryptophan proteins and soluble microbial byproducts and coenzyme F420 appeared in the three-dimensional fluorescence spectra of LB-EPS, and humic acid-like peaks appeared in TB-EPS. Chloroflexi was the dominant phylum under CIP stress, and its relative abundance were 43.13%, 44.48% and 39.96% when the CIP concentration in the influent were 3 mg/L, 5 mg/L and 10 mg/L, respectively. Methanobacterium and Methanothrix were the dominant genera of archaea, thus ensuring the efficient operation of the UASB reactor. KEGG functional analysis showed that archaea and bacteria were mainly carbohydrate metabolism and energy metabolism. After increasing CIP concentration, the functional gene abundance of membrane transport decreased greatly, from 10.15% to 9.31%, indicating that increasing CIP concentration had a greater impact on archaea.

Key words: anaerobic, ciprofloxacin, phosphorus, sludge characteristics, microbial community

CLC Number:  X703.1
[1] 罗丽芳. 污水生物脱氮除磷研究进展[J]. 生物化工, 2021, 7(2): 137-141. DOI: 10.3969/j.issn.2096-0387.2021.02.040.
[2] 卢宇翔, 农志文, 宿程远, 等. 微曝气-ABR处理养猪废水及微生物群落分布[J]. 广西师范大学学报(自然科学版), 2018, 36(4): 90-98. DOI: 10.16088/j.issn.1001-6600.2018.04.012.
[3] 张佩兰. 厌氧生物除磷及其微生物种群结构特性的研究[D]. 广州: 广州大学, 2011.
[4] DÉVAI I, FELFÖLDY L, WITTNER I, et al. Detection of phosphine: new aspects of the phosphorus cycle in the hydrosphere[J]. Nature, 1988, 333: 343-345. DOI: 10.1038/333343a0.
[5] KOVALAKOVA P, CIZMAS L, MCDONALD T J, et al. Occurrence and toxicity of antibiotics in the aquatic environment: a review[J]. Chemosphere, 2020, 251: 126351. DOI: 10.1016/j.chemosphere.2020.126351.
[6] TANG T T, LIU M, DU Y, et al. Deciphering the internal mechanisms of ciprofloxacin affected anaerobic digestion, its degradation and detoxification mechanism[J]. Science of the Total Environment, 2022, 842: 156718. DOI: 10.1016/j.scitotenv.2022.156718.
[7] YI K X, WANG D B, YANG Q, et al. Effect of ciprofloxacin on biological nitrogen and phosphorus removal from wastewater[J]. Science of the Total Environment, 2017, 605: 368-375. DOI: 10.1016/j.scitotenv.2017.06.215.
[8] 邹高龙, 刘志文, 董洁平, 等. 环丙沙星在污水处理过程中的迁移转化及对污水生物处理的影响[J]. 环境科学学报, 2019, 39(2): 308-317. DOI: 10.13671/j.hjkxxb.2018.0335.
[9] MAI D T, STUCKEY D C, OH S. Effect of ciprofloxacin on methane production and anaerobic microbial community[J]. Bioresource Technology, 2018, 261: 240-248. DOI: 10.1016/j.biortech.2018.04.009.
[10] CHEN H B, ZENG X N, ZHOU Y Y, et al. Influence of roxithromycin as antibiotic residue on volatile fatty acids recovery in anaerobic fermentation of waste activated sludge[J]. Journal of Hazardous Materials, 2020,394: 122570. DOI: 10.1016/j.jhazmat.2020.122570.
[11] 唐琳钦, 王安柳, 宿程远, 等. 不同氮源对好氧颗粒污泥理化特性及微生物群落影响[J]. 广西师范大学学报(自然科学版), 2021, 39(2): 144-153. DOI: 10.16088/j.issn.1001-6600.2020061301.
[12] YAN H, LI J Z, MENG J, et al. Effects of reflux ratio on the anaerobic sludge and microbial social behaviors in an expanded granular sludge bed reactor: from the perspective of acyl-homoserine lactones-mediated quorum sensing[J]. Bioresource Technology, 2021, 337: 125360. DOI: 10.1016/j.biortech.2021.125360.
[13] FAN Y M, LV M Y, NIU X J, et al. The key step of gaseous phosphorus release in anaerobic digestion[J]. Process Safety and Environmental Protection, 2020, 137: 238-245. DOI: 10.1016/j.psep.2020.02.035.
[14] YANG F X, ZHANG C S, RONG H W, et al. Research progress and application prospect of anaerobic biological phosphorus removal[J]. Applied Microbiology and Biotechnology, 2019, 103: 2133-2139. DOI: 10.1007/s00253-019-09634-0.
[15] 樊仪旻. 厌氧消化微生物产磷化氢的机理研究[D]. 广州: 华南理工大学, 2021.
[16] LU M Q, NIU X J, CHEN W Y, et al. Phosphine production in anaerobic wastewater treatment under tetracycline antibiotic pressure[J]. Journal of Enviromental Sciences, 2018, 69: 239-250. DOI: 10.1016/j.jes.2017.10.018.
[17] NIU X J, WEI A S, LI Y D, et al. Phosphine in paddy fields and the effects of environmental factors[J]. Chemosphere, 2013, 93: 1942-1947. DOI: 10.1016/j.chemosphere.2013.06.078.
[18] 周曼, 邓良伟, 杨红男, 等. 鸡粪中温干式沼气发酵启动阶段温度变化对产气性能的影响[J]. 农业环境科学学报, 2018, 37(8): 1785-1792. DOI: 10.11654/jaes.2018-0415.
[19] LIU W, NIU X J, CHEN W Y, et al. Effects of applied potential on phosphine formation in synthetic wastewater treatment by microbial electrolysis cell (MEC)[J]. Chemosphere, 2017, 173: 172-79. DOI: 10.1016/j.chemosphere.2017.01.006.
[20] YU H R, LAI B Y, YANG H Y, et al. In situ probing methanogenesis in anaerobic wastewater treatment using front-face excitation-emission matrix (FF-EEM) fluorescence[J]. Journal of Cleaner Production, 2023, 387: 135734. DOI: 10.1016/j.jclepro.2022.135734 .
[21] 郭博文, 许国芹, 柳静, 等. 厌氧发酵过程中辅酶F420浓度与产甲烷能力关系探究[J]. 云南师范大学学报(自然科学版), 2022, 42(6): 5-8. DOI: 10.7699/j.ynnu.ns-2022-068.
[22] SHAO Y X, ZHANG H X, BUCHANAN I, et al. Comparison of extracellular polymeric substance (EPS) in nitrification and nitritation bioreactors[J]. International Biodeterioration & Biodegradation, 2019, 143: 104713. DOI: 10.1016/j.ibiod.2019.06.001.
[23] STRIETH D, SCHWARZ A, STIEFELMAIER J, et al. New procedure for separation and analysis of the main components of cyanobacterial EPS[J]. Journal of Biotechnology, 2021, 328: 78-86. DOI: 10.1016/j.jbiotec.2021.01.007.
[24] WANG T, HUANG Z X, RUAN W Q, et al. Insights into sludge granulation during anaerobic treatment of high-strength leachate via a full-scale IC reactor with external circulation system[J]. Journal of Environmental Sciences, 2018, 64: 227-234. DOI: 10.1016/j.jes.2017.06.024.
[25] YIN Y J, SUN J, LIU F Y, et al. Effect of nitrogen deficiency on the stability of aerobic granular sludge[J]. Bioresource Technology, 2019, 275: 307-313. DOI: 10.1016/j.biortech.2018.12.069.
[26] ZHANG H Q, JIA Y Y, KHANAL S K, et al. Understanding the role of extracellular polymeric substances on ciprofloxacin adsorption in aerobic sludge, anaerobic sludge, and sulfate-reducing bacteria sludge systems[J]. Environmental Science & Technology, 2018, 52: 6476-6486. DOI: 10.1021/acs.est.8b00568.
[27] ZHANG S Q, ZHANG L Q, MADDELA N R, et al. Exposure to Cr(Ⅵ) affects partial denitrification process-nitrite accumulation, EPS characteristic and microbial community assembly[J]. Journal of Environmental Chemical Engineering, 2023, 11: 109001. DOI: 10.1016/j.jece.2022.109001.
[28] MENG L W, WANG J C, LI X K, et al. Microbial community and molecular ecological network in the EGSB reactor treating antibiotic wastewater: response to environmental factors[J]. Ecotoxicology and Environmental Safety, 2021, 208: 111669. DOI: 10.1016/j.ecoenv.2020.111669.
[29] NIU X J, WANG J F, WU H, et al. Matrix-bound phosphine in the paddy soils of South China and its relationship to environmental factors and bacterial composition[J]. Journal of Soils and Sediments, 2016, 16: 592-604. DOI: 10.1007/s11368-015-1258-4.
[30] LI P S, CHEN Q, DONG H, et al. Effect of applying potentials on anaerobic digestion of high salinity organic wastewater[J]. Science of the Total Environment, 2022, 822: 153416. DOI: 10.1016/j.scitotenv.2022.153416.
[31] HE L S, LI L H, YING L, et al.Bioaugmentation with methanogenic culture to improve methane production from chicken manure in batch anaerobic digestion[J]. Chemosphere, 2022, 303: 135127. DOI: 10.1016/j.chemosphere.2022.135127.
[32] DAI X Y, SU C Y, CHEN Z X, et al. Sulfonamide and quinolone antibiotics contaminated wastewater treatment by constructed rapid infiltration: efficiency and microbial community structure[J]. Process Safety and Environmental protection, 2022, 161: 542-555. DOI: 10.1016/j.psep.2022.03.056.
[33] TANG L Q, SU C Y, CHEN Y, et al. Influence of biodegradable polybutylene succinate and non-biodegradable polyvinyl chloride microplastics on anammox sludge: performance evaluation, suppression effect and metagenomic analysis[J]. Journal of Hazardous Materials, 2021, 401: 123337. DOI: 10.1016/j.jhazmat.2020.123337.
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