Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (6): 158-168.doi: 10.16088/j.issn.1001-6600.2022123001

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Effects of Ciprofloxacin on Operation Efficiency and Microbial Community Response Characteristics of Anaerobic Reactor for Treatment Phosphorus-Containing Wastewater

YANG Wenjing1, DENG Yulian1, CHEN Zhuxin1, TAO Afeng1, WEI Lixin1, WU Jinyan1, TIAN Yihao1, SU Chengyuan1,2*   

  1. 1. School of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China
  • Received:2022-12-30 Revised:2023-02-13 Published:2023-12-04

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Abstract: The presence of antibiotic pollutants in wastewater treatment process may affect the microbial activity in sludge, and the microbial activity directly affects the biological phosphorus removal effect of wastewater. Therefore, the effect of ciprofloxacin (CIP) on the operation efficiency of the anaerobic reactor treating high-concentration phosphorus wastewater and the response of microbial community were explored, and the COD removal rate, total phosphorus and phosphate removal rate, related enzyme activity and microbial community structure wereinvestigated. The results showed that the COD removal rate of high-concentration phosphorus wastewater in the reactor was stable at about 98% without CIP, and the average COD removal rate decreased to 63.1% when the CIP concentration was 10 mg/L, and the total phosphorus removal rate showed a downward trend in fluctuations. The addition of 10 mg/L CIP had a great effect on the concentration of phosphine; peaks associated with tryptophan proteins and soluble microbial byproducts and coenzyme F420 appeared in the three-dimensional fluorescence spectra of LB-EPS, and humic acid-like peaks appeared in TB-EPS. Chloroflexi was the dominant phylum under CIP stress, and its relative abundance were 43.13%, 44.48% and 39.96% when the CIP concentration in the influent were 3 mg/L, 5 mg/L and 10 mg/L, respectively. Methanobacterium and Methanothrix were the dominant genera of archaea, thus ensuring the efficient operation of the UASB reactor. KEGG functional analysis showed that archaea and bacteria were mainly carbohydrate metabolism and energy metabolism. After increasing CIP concentration, the functional gene abundance of membrane transport decreased greatly, from 10.15% to 9.31%, indicating that increasing CIP concentration had a greater impact on archaea.

Key words: anaerobic, ciprofloxacin, phosphorus, sludge characteristics, microbial community

CLC Number:  X703.1
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