Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (2): 175-189.doi: 10.16088/j.issn.1001-6600.2021111702

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Structural Characteristics of Environmental Microbial Community Studied by High-throughput Sequencing Technology

XIAO Fei1,2, DING Xusheng1, FAN Peipei1, WANG Weihong1*   

  1. 1. College of Water Conservancy and Civil Engineering, Xinjiang Agricultural University, Urumqi Xinjiang 830052, China;
    2. College of Water Resources and Architectural Engineering, Tarim University, Aral Xinjiang 843300, China
  • Received:2021-11-17 Revised:2022-01-12 Online:2023-03-25 Published:2023-04-25

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Abstract: High-throughput sequencing technology was used to analyze the evolution and diversity of microbial community structure in sequence batch reactors (R1, R2), the correlation between bacterial community and tomato sauce wastewater quality and the prediction of microbial enzymes. The results showed that there were significant differences in the level of phylum, class and genus. Proteobacteria and Bacteroidetes were the dominant phylum at the level of phylum. At class level, Betaproteobacteria, Alphaproteobacteria and Bacteroidia were the dominant bacteria. At the genus level, Zoogloea and Flavobacterium were the dominant genera. From the initial stage of particle formation to the stable stage, the abundances of Proteobacteria, Bacteroidetes, Betaproteobacteria, Alphaproteobacteria, Bacteroidia, Zoogloea and Flavobacterium in R1 and R2 were 1.75%, -7.20%, 35.49%, 0.12%, -15.23%, -3.11% and -5.23% respectively. The abundance of 1.78%, -2.00%, 33.11%, -0.54%, -14.80%, -2.87% and 6.13% showed little variation. But the differences in abundance was found in Alphaproteobacteria, Bacteroidia and Flavobacterium. Correlation analysis showed that Sphingobacteriia and Negativicutes had a significant negative correlation with NH3-N and PO3-4-P, Flavobacteria had a significant negative correlation with COD. Gammaproteobacteria and Clostridia were significantly positively correlated with COD and NH3-N, while Betaproteobacteria was significantly positively correlated with PO3-4-P. Proteobacteria has the effect of nitrogen removal, Bacteroidetes is related to the degradation of EPS, Acidobacteria has a positive effect on the initial coagulation of flocs sludge. Anaerolineae also exists in the sludge at the floc stage, and Anaerolineae is beneficial to the settlement of the floc sludge. Among them delta-9 desaturase (stearoyl-CoA dehydrogenase (ó-9 dehydrogenase) and transport system permease protein (system permease protein) are more abundant, and lactocepin (lactocerin), linoleoyl-CoA desaturase (sub oleoyl-CoA dehydrogenase) and ribosomal protein S6 modification protein (ribosomal protein S6 modification protein) changed most significantly, but microbial enzymes throughout the system maintained similar functions.

Key words: aerobic granular sludge, high-throughput technology, microbial flora, tomato paste wastewater, microbial enzyme

CLC Number: 

  • X703
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