Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (2): 161-174.doi: 10.16088/j.issn.1001-6600.2022020901

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Effects of phzR Gene of Pseudomonas aeruginosa on Biofilm Gene Expression and Cell Motility

DAI Mingyao1, LI Yashi1, HUANG Xinni1, XIAO Jun1, HUANG Zhiqing1, LÜ Chunmeng1, LU Zujun1,2*   

  1. 1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China
  • Received:2022-02-09 Revised:2022-05-30 Online:2023-03-25 Published:2023-04-25

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Abstract: In order to prognosis the biosecurity of PA2019NES which is with high yield of phenazines in agricultural exploitation, the real-time fluorescence quantitative PCR was applied to test the difference of transcriptional volume of 9 genes which were associated with biofilm, quorum sensing system and type Ⅵ secretory system, to reveal the transcriptional relationship between phzR and the above mentioned 9 genes, as well as its effect on the cells motility, especially the differeces between PA2016NX1 and PA2019NES, which was with multiple copies of phzR gene, were in biofilm and planktonic state, respectively. The results showed that, in the planktonic state, multiple copies phzR gene up-regulated the mRNA level of vgrG and algD genes (P<0.05), the mRNA level of pilA was down-regulated (P<0.05); however, the mRNA level of the other 6 genes was not significantly affected (P>0.05). In the biofilm state, the mRNA level of rhlI gene was up-regulated (P<0.05), but that of fimX and wspR genes was down-regulated (P<0.05) and that of the other 6 genes was not significantly affected (P>0.05). After 24 h culture, there was no significant difference in twitching ability between PA2016NX1 and PA2019NES (P>0.05), but the swimming, ability of PA2019NES was stronger than that of PA2016NX1 (P<0.05), however, the swarming ability of PA2019NES was weaker than that of PA2016NX1 (P<0.05). These results suggested that the power of conditional pathogenicity of PA2019NES may not be lower than that of PA2016NX1, hence, when PA2019NES was developed for agricultural use, the spread of live bacteria to the environment should be prevented.

Key words: Pseudomonas aeruginosa, bacterial biofilm, quorum sensing system, type Ⅵ secretion system, phzR gene

CLC Number: 

  • Q933
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