毛红椿皮的化学成分及生物活性研究

doi:10.16088/j.issn.1001-6600.2021090102

Abstract

Abstract: In order to study the chemical constituents and biological activities from the bark of Toona ciliata var. pubescens, in this paper, silica gel column chromatography, Sephadex LH-20, reversed-phase ODS C₁₈ and MCI, semi preparative HPLC and other chromatographic techniques were used to isolate and purify the natural compounds; AGAR dilution method was used to screen the bactericidal activity of plant pathogens, and the antitumor activity was screened by MTT method. Twenty compounds were isolated from the ethyl acetate extract of 95% ethanol extract of the bark of Toona ciliata var. pubescens, and were identified to be toonacilin (1), odoratone (2), bourjotinolone A (3), dyvariabilin H (4), piscidinol A (5), piscidinol B (6), 3-episapelin A (7), 8(14), 15-isopimaradiene-2α,3α,18-triol (8), labd-13-ene-8α,15-diol (9), nidorellol (10), catechin (11), 1-linolenoylglycerol (12), tianshic acid (13), (-)-neoechinulin A (14), aurantiamide acetate (15), dibutylphthalate (16), 4,7-dimethoxy-5-methylcoumarin (17), scopoletin (18), (22E)-5a,8a-epidioxyergosta-6,22-dien-3b-ol (19), β-sitosterol (20), respectively, based on physicochemical properties and spectral analysis techniques.Except forcompounds 4, 11, 16~18, 20 ,the remaining compoundswere isolated from this plant for the first time.The results of antibacterial activity showed the compound 1 had potential inhibitory effect on Aternaria solani, Botrytis cinerea and Phytophthora capsici at a concentration of 50 mg/L, whilecompound 4 on Botrytis cinerea was 76.5% at a concentration of 50 mg/L . Compound 2 showed strong inhibition against human breast cancer cell line MDA-MB-231 (IC₅₀=12.9 μmol/L ), andcompound5 showed strong inhibition against HEL cellwith IC₅₀ value of 8.4 μmol/L. Compound 3 had strong inhibitory effects on human breast cancer cell line MDA-MB-231, human rhabdomyoma cell line A-673 and human erythrocytic leukemia cell line HEL, with the IC₅₀ values from 8.2 to 11.2 μmol/L.

Key words: Toona ciliata var. pubescens, chemical constituents, triterpene, antibacterial activity, anti-tumor activity

CLC Number:

R284

DU Libo, LI Jinyu, ZHANG Xiao, LI Yonghong, PAN Weidong. Chemical Constituents and Biological Activity from the Bark of Toona ciliata var. pubescens[J].Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(4): 162-172.

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Chemical Constituents and Biological Activity from the Bark of Toona ciliata var. pubescens

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