Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (2): 95-100.

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Inhibitory Action of a Xanthono-pyridine Derivative XP-16 on Human Nasopharyngeal Carcinoma CNE Cells in vitro

HAN Liu-yu1,2, DAI Zhi-kai3, YANG Zheng-min1,2, HUANG Jun1,2, QIN Jiang-ke1,2, SU Gui-fa1,2   

  1. 1. College of Chemistry and Pharmaceutical Science, Guangxi Normal University, Guilin Guangxi 541004, China;
    2. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of State Education Ministry, Guangxi Normal University, Guilin Guangxi 541004, China;
    3. College of Pharmaceutical Science, Guilin Medical University, Guilin Guangxi 541004, China
  • Received:2013-11-15 Online:2014-06-25 Published:2018-09-25

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Abstract: MTT assay, morphological examination and colonial assay were applied to evaluate the antiproliferative effect of a new xanthono-pyridine derivative (XP-16) on human nasopharyngeal carcinoma CNE cells; Hoechest 33258/PI double staining, flow cytometry, spectrofluorimetry and RT-PCR were also employed to investigate its possible action mechanism. The experiment results showed that XP-16 could inhibit the proliferation of CNE cells in dose- and time-dependent manner. Typical apoptotic morphology such as chromatin aggregation and nuclear fragmentation was observed in XP-16 treated CNE cells for 24 h in a dose-dependent manner. After treated with XP-16, CNE cells were blocked in G2-M and S phases, mitochondria membrane potential of the cells was decreased, relative Bad and MT-1A mRNA level was up-regulated. The apoptosis of CNE cells inducing by XP-16 might be associated with decreasing mitochondria membrane potential and up-regulating MT-1A.

Key words: xanthone, antitumor, human nasopharyngeal carcinoma cell line CNE, apoptosis

CLC Number: 

  • R962
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