Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (2): 88-94.

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Optimization of Silk Fibroin Extraction by Alkali Degumming and Spectra of Its Conformation in Solution

CHENG Lei1,2, XIN Wei-biao1, LIU Zhi-xu1, WANG Zhuo-yuan1, SHEN Xing-can1   

  1. 1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, Guangxi Normal University, Guilin Guangxi 541004, China;
    2. Science and Technology Department, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2013-10-11 Online:2014-06-25 Published:2018-09-25

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Abstract: Degumming conditions of silkworm cocoon produced in Guangxi province were optimized by the method of alkali degumming. The degumming rates of silkworm were checked by the carmine of picric acid and SEM. It is showed that the silkworm can be degummed clearly with the condition of 30 min, two times and 1:200 of bath ratio for degumming. Silk fibroin can be prepared by dissolution and degumming of three solvents of CaCl2-EtOH-H2O, LiBr-H2O and LiBr-EtOH-H2O. The conformation of silk fibroin was characterized by the optical spectroscopy of Raman and IR. The silk is mainly the conformation of β-folding by alkali degumming. After dissolving and degumming the silk, the random coil is mainly the conformation of silk fibroin. Comparing with three dissolving systems, it is showed that alcohol is better for the formation of the conformation of the random coil for silk fibroin, and Li+ has more influence than Ca2+ for secondary structure of silk fibroin.

Key words: alkali degumming, silk fibroin, IR spectra, Raman spectra, conformation

CLC Number: 

  • TQ321.4
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