高产四甲基吡嗪菌株Bacillus sp. TTMP20的分离及其基因组分析

doi:10.16088/j.issn.1001-6600.2026012903

Abstract

Abstract: Tetramethylpyrazine is a pharmaceutically active substance and a flavor substance in Baijiu. A strain (Bacillus sp. TTMP20) with a high tetramethylpyrazine yield (3 896.36 mg/L) was screened from Daqu and fermented grains of Danquan Baijiu in this study. To better understand the mechanism of tetramethylpyrazine production by TTMP20 and perform its genetic modification, genome sequencing of TTMP20 was conducted. This study described the genomic characteristics of TTMP20. It analyzed genes and pathways associated with tetramethylpyrazine synthesis in detail. Gene function was analyzed by KEGG, COG, GO, NR and CAZy annotation. These pathways included fructose metabolism pathway, pathways of starch and sucrose metabolism, glycolysis pathway, pentose phosphate pathway, partial butanoate metabolism pathway and nitrogen metabolism pathway. Results showed that Bacillus sp. TTMP20 had complete metabolic pathways for acetoin and tetramethylpyrazine, and TTMP20 could utilize multiple complex carbon sources. Genome information thoroughly revealed the connection between genes and tetramethylpyrazine production.

Key words: tetramethylpyrazine, Bacillus sp. TTMP20, genome sequencing, metabolic pathways, active substances, flavor compounds

CLC Number: Q933

Li Yujia, Ou Jianmei, Yu Shisong, Shang Changhua. Isolation and genomic analysis of a high tetramethylpyrazine yield Bacillus sp. TTMP20[J].Journal of Guangxi Normal University(Natural Science Edition), 2026, 44(6): 257-267.

References

[1] Mcgovern P E, Zhang J Z, Tang J G, et al. Fermented beverages of pre- and proto-historic China[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(51): 17593-17598. DOI:10.1073/pnas.0407921102.
[2] Zheng X W, Han B Z. Baijiu (白酒), Chinese liquor: history, classification and manufacture[J]. Journal of Ethnic Foods, 2016, 3(1): 19-25. DOI:10.1016/j.jef.2016.03.001.
[3] Jin G Y, Zhu Y, Xu Y. Mystery behind Chinese liquor fermentation[J]. Trends in Food Science & Technology, 2017, 63: 18-28. DOI:10.1016/j.tifs.2017.02.016.
[4] 季克良, 郭坤亮.剖读茅台酒的微量成分[J]. 酿酒科技, 2006(10): 98-100.
[5] Yan Y, Chen S, Nie Y, et al. Quantitative analysis of pyrazines and their perceptual interactions in soy sauce aroma type Baijiu[J]. Foods, 2021, 10(2): 441. DOI: 10.3390/foods10020441.
[6] Shi X S, Zhao S M, Chen S X, et al. Tetramethylpyrazine in Chinese Baijiu: presence, analysis, formation, and regulation[J]. Frontiers in Nutrition, 2022, 9: 1004435. DOI:10.3389/fnut.2022.1004435.
[7] Meng Z Y, Chen H Z, Meng S X. The roles of tetramethylpyrazine during neurodegenerative disease[J]. Neurotoxicity Research, 2021, 39(5): 1665-1677. DOI: 10.1007/s12640-021-00398-y.
[8] Zhang Y F, He L F, Ma C, et al. Research progress on the pharmacy of tetramethylpyrazine and its pharmacological activity in cardiovascular and cerebrovascular diseases[J]. Journal of Pharmacy and Pharmacology, 2022, 74(6): 843-860. DOI: 10.1093/jpp/rgac015.
[9] Qi M Z, Su X H, Li Z H, et al. Bibliometric analysis of research progress on tetramethylpyrazine and its effects on ischemia-reperfusion injury[J]. Pharmacology & Therapeutics, 2024, 259: 108656. DOI:10.1016/j.pharmthera.2024.108656.
[10]Zhao Y K, Liu Y, Chen K J. Mechanisms and clinical application of tetramethylpyrazine (an interesting natural compound isolated from Ligusticum wallichii): current status and perspective[J]. Oxidative Medicine and Cellular Longevity, 2016, 2016: 2124638. DOI: 10.1155/2016/2124638.
[11]Feng F, Xu D Q, Yue S J, et al. Neuroprotection by tetramethylpyrazine and its synthesized analogues for central nervous system diseases: a review[J]. Molecular Biology Reports, 2024, 51: 159. DOI: 10.1007/s11033-023-09068-y.
[12]Kosuge T, Kamiya H. Discovery of a pyrazine in a natural product: tetramethylpyrazine from cultures of a strain of Bacillus subtilis[J]. Nature, 1962, 193(4817): 776. DOI: 10.1038/193776a0.
[13]Zhu B F, Xu Y, Fan W L. High-yield fermentative preparation of tetramethylpyrazine by Bacillus sp. using an endogenous precursor approach[J]. Journal of Industrial Microbiology & Biotechnology, 2010, 37(2): 179-186. DOI: 10.1007/s10295-009-0661-5.
[14]徐岩, 吴群, 范文来, 等. 中国白酒中四甲基吡嗪的微生物产生途径的发现与证实[J]. 酿酒科技, 2011(7): 37-40. DOI: 10.13746/j.njkj.2011.07.001.
[15]徐岩. 基于风味导向技术的中国白酒微生物及其代谢调控研究[J]. 酿酒科技, 2015(2): 1-11,16. DOI: 10.13746/j.njkj.2014533.
[16]王庆, 王超彦, 徐海林, 等. 高温大曲中高产四甲基吡嗪芽孢杆菌的分离鉴定及发酵条件优化[J]. 中国酿造, 2022, 41(10): 125-129.
[17]邱勇, 王颖, 张煨, 等. 高产四甲基吡嗪的菌株筛选及其麸曲制作工艺优化[J]. 中国酿造, 2023, 42(4): 112-117.
[18]史洪丽, 余梦婷, 王世昕, 等. 高温大曲中高产四甲基吡嗪芽孢杆菌的筛选及其在酒醅堆积中的应用[J]. 中国酿造, 2025, 44(3): 59-65.
[19]范恩帝, 黄魏, 谢丹, 等. 酱香大曲中高产四甲基吡嗪微生物的选育及应用研究[J]. 酿酒科技, 2022(11): 45-50,71. DOI: 10.13746/j.njkj.2022300.
[20]张锦华, 张潇月, 白宝清, 等. 清香型白酒大曲中高产四甲基吡嗪菌株的筛选、鉴定及发酵培养基优化[J]. 中国酿造, 2023, 42(3): 47-52.
[21]郭春生, 杨舟, 乔月梅, 等. 高产四甲基吡嗪微生物的筛选、鉴定及发酵条件优化[J]. 中国酿造, 2022, 41(9): 80-85.
[22]钟桂芳, 张帆, 郭辉祥, 等. 高温大曲中产四甲基吡嗪细菌的筛选及鉴定[J]. 中国酿造, 2020, 39(8): 107-111.
[23]刘国红, 林乃铨, 林营志, 等. 芽孢杆菌分类与应用研究进展[J]. 福建农业学报, 2008(1): 92-99. DOI: 10.19303/j.issn.1008-0384.2008.01.020.
[24]鲁倩, 唐平, 王晓丹, 等. 白酒酿造过程中芽孢杆菌的研究进展[J].中国酿造, 2025, 44(5): 26-31.
[25]罗会颖, 姚斌, 范云六. 嗜酸糖苷水解酶研究进展[J]. 中国农业科技导报, 2013, 15(5): 1-7.
[26]李雨佳, 罗丽蓉, 尚常花. 微生物发酵生产四甲基吡嗪方法综述[J]. 广西师范大学学报(自然科学版), 2024, 42(4): 32-40. DOI: 10.16088/j.issn.1001-6600.2023092201.
[27]Xu Y Q, Xu C Y, Li X T, et al. A combinational optimization method for efficient synthesis of tetramethylpyrazine by the recombinant Escherichia coli[J]. Biochemical Engineering Journal, 2018, 129: 33-43. DOI:10.1016/j.bej.2017.10.010.

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Isolation and genomic analysis of a high tetramethylpyrazine yield Bacillus sp. TTMP20

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