Journal of Guangxi Normal University(Natural Science Edition) ›› 2024, Vol. 42 ›› Issue (1): 1-8.doi: 10.16088/j.issn.1001-6600.2023042406

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Advances in Cytoplasmic Delivery Strategies for Non-Endocytosis-Dependent Biomolecules

YUAN Jingjing1, ZHENG Yuzhao1, XU Chenfeng2*, YIN Tingjie1*   

  1. 1. School of Pharmacy, China Pharmaceutical University, Nanjing Jiangsu 210000, China;
    2. Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei 430022, China
  • Received:2023-04-24 Revised:2023-06-19 Online:2024-01-25 Published:2024-01-19

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Abstract: Biomacromolecular drugs have become an important component of the new generation of therapeutic drugs due to their high efficiency and specificity. However, their molecular stability are poor, and they are prone to enzymatic hydrolysis, and are difficult to cross biofilms. The effectiveness of traditional nano delivery strategies for biomacromolecule drugs is mainly limited by the low escape efficiency of lysosome. It is of great significance to develop endocytosis independent direct cytoplasmic delivery strategies. This paper reviews the effect mechanism and research progress of cell penetrating peptide, low pH insertion peptide, scavenger receptor mediated non endocytosis, membrane fusion, endoplasmic reticulum pathway, mercaptan mediated, non endosome capture based intracellular delivery strategy of biomacromolecule drugs based on liquid-liquid phase separation technology, and analyzes the difficulties in technology transformation.

Key words: biomacromolecular drugs, cell penetrating peptide, mercaptol mediated entry into cells, endoplasmic reticulum pathway, membrane fusion

CLC Number:  R943
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