Journal of Guangxi Normal University(Natural Science Edition) ›› 2023, Vol. 41 ›› Issue (3): 155-162.doi: 10.16088/j.issn.1001-6600.2022042901

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Origin and Functional Verification of IL-10 in Tumor Tissue of Lung Cancer Mice

HE Liu1,2,3, LIN Chao1,2,3, LI Peihua1,2,3, CHENG Zhixiu1,2,3, ZHU Yu1,2,3, PU Shiming1,2,3, ZHOU Zuping1,2,3*   

  1. 1. School of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Guangxi University Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541004, China;
    3. Research Center for Biomedical Sciences, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2022-04-29 Revised:2022-06-06 Online:2023-05-25 Published:2023-06-01

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Abstract: To investigate the expression and source of interleukin-10 (IL-10) in lung cancer tumor tissues, and to investigate the effect of IL-10 on tumors. The levels of IL-10 in the serum of Lewis lung carcinoma mouse model were detected by MSD hypersensitive electrochemiluminescence assay, the main source of IL-10 in tumor tissues was analyzed using flow cytometry, and the effects of IL-10 recombinant protein on proliferation, apoptosis and migration of lung cancer cells were examined by CCK-8 assay, annexin V-propidium iodide (annexin V-PI) apoptosis assay and scratch wound assay. The results showed that the serum levels of IL-10 were significantly increased in Lewis lung carcinoma mice compared with normal mice, and IL-10 in tumor tissues was mainly produced by immune cells, including B cells and myeloid-derived suppressor cells (MDSCs), which were the main source of IL-10, and the recombinant protein of IL-10 could significantly inhibit the proliferation and migration and promote the apoptosis of LLC (mouse Lewis lung carcinoma line). IL-10 level was closely related to lung cancer, B cells and MDSCs were the main source of IL-10 production, and IL-10 may play an antitumor role by activating CD8 T cells or directly acting on tumor cells.

Key words: lung cancer, IL-10, cell origin, function

CLC Number:  R734.2;Q28
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