Abstract: An ultra-performance liquid chromatography-quadropole mass method was established to simultaneously quantify twelve constituents in the extracts of Folium ficim icrocarpae. The extracts were extracted with 10% CH₃CN by ultrasonic extraction. The extracts were analyzed in multiple-reaction monitoring mode with an ultra-performance liquid chromatography-tandem mass spectrometry equipped with electrospray ionization and an Agilent ZORBAX HSS T3 column(2.1 mm×100 mm,1.8 μm)using gradient MeOH-0.1% formic acid water as mobile phase. The area of the analytes showed good linearity with respect to the analyte concentrations(R²≥0.99). The limits of quantification for all of the twelve analytes were less than 1.694 μg/L and the recovery rates of the method were 82.2%～109.5%. The inter-day and intra-day relative standard deviations were 1.0%～6.0% and 1.5%～5.1%, respectively. The results showed that the extracts contained large amounts of quinic acid, gallic acid, isovitexin, vitexin, orientin, isoorientin constituents. The established method could be used asscientific evidence for the revision of the quality control of Keteling preparation.

Key words: extracts of Folium ficim icrocarpae, Keteling preparation, UPLC-QQQMS, quantification, flavonoid, phenolics